Objective To evaluate the effect of ultrasonic speckle technique on cardiac function in patients with acute myocardial infarction (AMI).Methods 30 patients with AMI were selected as observation group,30 healthy people were selected as control group,and they were all detected by ultrasonic speckle technique.The differences of measurement indices of cardiac function with ultrasonic speckle technique were compared between the observation group and control group.The difference between ultrasonic speckle technique and its measured value was observed with single photon emission computed tomography(SPECT) as the gold standard.The correlation between the results of three-dimensional speckle ultrasound and SPECT in patients with AMI was analyzed.Results The parameters of LVEDV [(62.2 ± 6.4) mL vs.(80.3 ± 7.5) mL],LVESV [(21.7 ± 5.6) mL vs.(35.4 ± 7.2) mL] and LVEF [(62.6 ± 5.9)％ vs.(72.8 ± 5.6)％] in the observation group were significantly lower than those in the control group,the IVSTd [(1.1 ± 0.2) cm vs.(0.9 ± 0.2) cm] and LVPWd [(1.2 ± 0.2) cm vs.(0.8 ± 0.1) cm] were significantly higher than those in the control group,the differences were statistically significant(t =8.431,9.865,3.651,8.798,6.654,all P ＜ 0.05).In AMI patients,there were no significant differences in left ventricular parameters LVEDV,LVESV,LVEF measured between ultrasonic speckle technique and SPECT(all P ＞ 0.05).With SPECT as the gold standard,in AMI patients,the ultrasound parameters LVEDV,LVESV and LVEF were positively correlated with SPECT parameters(r =0.912,0.903,0.899,all P ＜ 0.05).Conclusion Ultrasound speckle technique can well evaluate myocardial function in patients with myocardial infarction.

Objective Explore the changes of serum interleukin(IL) levels and its clinic significance in patients with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP).Methods The levels of IL-2, IL-4 and IL-6 in serum of 34 patients with DEACMP were measured by ELESA before and after treatment. As control, the levels of IL-2, IL-4 and IL-6 in 30 healthy persons and 30 patients with acute carbon monoxide poisoning(ACMP) were measured at same time.Results ⑴The levels of IL-2,IL-4 and IL-6 in serum of DEACMP patients before treatment were significantly lower than those after treatment(allP

Objective To monitor lung growth rule of normal middle-late pregnancy fetal. Methods 599 cases of 18 and 40 weeks normal singletons were grouped by every two weeks. The length of right lung in the sagittal plane and the cross-sectional plane were measured, and the growth curve was described. The ratio of right lung area (RLa) and head circumference (Hc),chest circumference (Tc) and of the chest area (Ta) in different gestational ages was calcalated. The correlation coefficient was obtained by applying statistical analysis. Results There was a linear relationship between RLa/Hc and RLa/Tc and growth of gestational age;the regression equation was:(RLa/Hc: ?Y= 0.948X-1.221, r2 =0.898, the RLa/Tc: ?Y=0.944X-1.800, r2 =0.894). RLa/Ta in different ges-tational age was in constant range:0.42~0.55. The length of right lung in the sagittal plane and the cross-sectional plane increased along with the gestational age, and the growth speed was:before and after diameter(the cross-sec-tional plane) > upper and lower diameter (sagittal plane) > about size (the cross-sectional plane). Conclusion Prenatal ultrasound can evaluate the growth rule of normal fetal lung and provide the basis for assessment of fetal lung maturity.

Objective To explore the effect of focused nursing model on negative emotions and subjective well-being of lung cancer patients. Methods Eighty-six lung cancer patients hospitalized from November 2014 to November 2016 in our hospital were divided into two groups according to the order of admission in equal number. The control group was treated with routine care, and the observation group was with focused nursing model. The two groups were compared in terms of anxiety by SAS, depression by SDS, subjective well-being. Results The scores by SAS and SDS in the observation group were lower than those in the control group (P<0.05). The levels of objective support, subjective support, self-evaluation and subjective well-being index of the observation group were higher than the control group (P <0.05). Conclusion Focused nursing model can effectively relieve negative emotions, improve subjective well-being of lung cancer patients, worthy of clinical promotion.

0.05). According to the logistic regression, the OR of age was 1.128 which showed that when age increase 1 year, the possibility of contracting CP was higher. The OR of genotype was 0.187 and 0.421, which showed that people with XX genotype were also more susceptible than those with Xx and xx genotype, when the other conditions were invariable. Conclusion:Specific relationship is found between the susceptibility of severe CP and the ER gene-XbaI polymorphism. People with XX genotype are most susceptive to CP.

ve To establish a method for determination of arsenic in drinking water. Methods The contents of arsenic in water samples were determined directly by graphite furnace atomic absorption spectrophotome-try with masking agent of palladium. The working conditions were defined as wave length of 193.7 nm, slit width of 1.3 nm, current of hollow cathode lamp of 12.0 mA; drying 40 sec at 80℃~140℃ ; ashing 20 sec at 1100℃~1200℃; atomizing 5 sec at 2 300℃; purifying 4 sec at 2 600℃ . Results The linear relationship was 10~200?g/ L ar-senic. The average recovery rates of water samples at concentrations of 50 ?g/ L and 150?g/ L arsenic after being added arsenic standard solutions were 100.7% and 103.6% respectively, and sensitivity reached 10?g/ L. The cor-relation coefficient were 0.9954~0.999 7 obtained from 6 randomly repeated determination of standard curve. The metal ions coexisting in determined water samples didn't interfere the determination of arsenic under the application of common uncoated graphite tube. The 0.2% aluminum didn't disturb the determination of arsenic also. Conclusion This method could be applied in the determination of arsenic in water, especially could be applied in the direct deter-mination of arsenic in more clear water samples.

Objective To study the anti-tumor effect of 5-aza-2′-deoxycytidine(5-aza-dC) on breast carcinoma xenografted in nude mice. Methods The model of breast carcinoma xenografted in nude mice was established.Ten mice were randomized into the treatment group(treated with 5-aza-dC) and control group(treated with PBS).The mass of the tumors before and after treatment were measured in the two groups,and the inhibition rate of the tumor was calculated and the growth curve was drawn.Immunohistochemistry and Western blotting were employed to detect the expression of normal epithelial cell specific-1(NES1)gene. Results The inhibition rate of the tumor in the treatment group was 57.44%,which was significantly different from the control group(P

Objective To investigate the current application of teaching cadavers and attitudes of medical students towards body donation and anatomy practicum and to make proposals on teaching and learning of human anatomy.Methods A on the spot questionnaire survey among 300 students studying in Hebei Medical University was conducted and the acquired data were contrasted analyzed.Results The teaching cadavers were insufficient and the quality of anatomy education was directly affected.Medical students took unfavorable attitudes towards anatomy practice and knew little about body donation.The main factors affected body donations were traditional concepts,lack of humanistic concern and complicated procedures of donation.Conclusions The short of teaching cadavers would be relieved by enhancing propaganda and legislation,emphasizing humanistic concern,simplifying procedures of donation.By means of anatomy teaching reformations,the learning interest and humanistic literacy of medical students are supposed to be improved.

Objective To investigate the role of heat shock protein 70 (HSP70) in liver flbrosis in infants with biliary atresia (BA) and its impact on prognosis. Methods Fourty-six (46) cases of infants with BA undergoing elective Kasai surgery were selected. In the same period, 30 cases of children with choledochal cyst and 17 cases of children with portal vein cavernous transformation were selected. The expressions of HSP70 in liver tissues were detected using immunohistochemical staining. The liver flbrosis in children with BA was detected using Sirius red-saturated picric acid staining. The expressions of HSP70 proteins in different flbrotic liver tissues were detected by using double staining. All postoperative BA infants were followed up and ended at June 30, 2016.Results The proportion of high expression of HSP 70 proteins in BA infants were signiflcantly higher than that in children with choledochal cyst and vein cavernous transformation (P<0.05). Rank correlation analysis showed that the expressions of HSP70 in liver tissues were positively correlated with the degree of liver flbrosis (r=0.861,P<0.05). 15 patients died among 46 cases of BA infants. The survival rate in BA children with mild liver flbrosis was 82.4%, which was signiflcantly higher than 58.6% in the severe group (P<0.05). The survival rate in HSP70 protein low expression group was 85.0%, while in HSP70 protein high expression group was 53.8%. Kaplan-meier survival analysis showed that the median survival time in the HSP70 protein low expression group was (34.0±2.6) months, while in the HSP70 protein high expression group was (18.3±2.2) months, the difference was statistically signiflcant (χx2=4.765,P=0.029).Conclusions The expressions of HSP70 proteins in liver tissues in infants with BA were high and were positively correlated with the degree of hepatic flbrosis. It suggested the possible involvement of HSP70 in the process of liver fibrosis. The upregulated expressions of HSP70 often indicated poor prognosis in children. It could be used as determining biomarker for prognosis.

Objective To compare the risk of tumor invasion and metastasis under paravertebral block (PVB) combined with general anesthesia versus general anesthesia in the patients undergoing radical resection for lung cancer performed via video-assisted thoracoscope in terms of plasma concentrations of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9).Methods Forty ASA physical status Ⅰ or Ⅱ patients of both sexes,aged 30-64 yr,with body mass index of 18-25 kg/m2,scheduled for elective radical resection for lung cancer performed via video-assisted thoracoscope,were randomly divided into 2 groups (n =20 each) using a random number table:general anesthesia group (group G) and PVB combined with general anesthesia (group PG).PVB of T4-7 was performed successfully with local injection of 0.375％ ropivacaine 5 ml before induction of anesthesia.Double-lumen endotracheal tube was placed after induction of anesthesia,and the patients were mechanically ventilated.Anesthesia was maintained with inhalation of sevoflurane (end-tidal concentration 1％-2％),and intravenous infusion of remifentanil 0.2-0.3 μg · kg-1 · min-1,and intermittent intravenous boluses of atracurium.Before anesthesia and at 24 h after surgery,the venous blood samples were collected for measurement of plasma concentrations of VEGF and MMP-9.Results The plasma VEGF and MMP-9 concentrations were significantly lower after surgery in group PG than in group G.Conclusion PVB combined with general anesthesia significantly decreases the risk of tumor invasion and metastasis in the patients undergoing radical lung cancer resection performed via video-assisted thoracoscope in comparison to general anesthesia.