Bibliometrics ; Double-Blind Method ; Drugs, Chinese Herbal ; therapeutic use ; Evaluation Studies as Topic ; Humans ; Medicine, Chinese Traditional ; Periodicals as Topic ; Phytotherapy ; Randomized Controlled Trials as Topic ; methods

This article explains the purpose of setting up the sperm bank, the role of the sperm bank in auxiliary reproduction technology, and exhaustively clarifies the ethical problems in the various aspects of the social life that are caused by the sperm bank, and finally it also proposes some relevant suggestions to these problems.

Due to therapeutic regimens for patients with malignant lymphoma (ML) is currently unsatisfactory, it is important to develop new complementary therapies for longer disease-free survival. Dendritic cells (DC) are specialized antigen-presenting cells of immune system and vaccination with tumor antigen-pulsed DC has been applied to treat patients for ML. In this review, we summarize various types of ML-associated antigens and clinical trials on DC-based immunotherapy in ML, and discuss the development of DC immunotherapy for ML patients in future.

It had fertile countryside land,rich products,dense population and creeks and lakes in ancient Hangzhou and its neighbour area,where there is a good place for planting medicinal materials.It had long history of planting medicinal materials with abundant output,pure and good nature,and much varieties;among which,some famous and costly medicinal materials,such as "eight Zhe's" and "eighteen Hangs",were not only adopted broadly by court,royalty and doctors,but purchased by many businessmen and sold far to abroad as well;they seemed to be in the leading place in China.

Objective To study the psychological nursing on the rehabilitation effect of patients with acute coronary syndrome(ACS)accompanied by depression.Methods 126 ACS patients were divided into the nursing group(64 cases)and the control group(62 cases)randomly.The control group adopted roUtine rehabilitative nursing measurs.While the nursing group received canonical and selective psychological nursing.The Zung Self-rating Depression Scale(SDS)and hamilton depression scale(HAMD)were used to evaluate the their psychological status.The electrocardiogram,clinical symptom and cardiovascular complications were observed and compared.Results The SDS and HAMD scores were different between the nursing group and the control group(P＜0.01)5 weeks after rehabilitative nursing but not before it.The ischemic changes of electrocardiogram,incidence frequency of angina pectoris and cardiovascular complications were significantly decreased in the nursing group compared with the control group(P＜0.05).Conclusion Psychological nursing could not only alleviate the depression of patients with ACS but also could improve the clinical symptoms of these patients and decrease the incidence frequency of cardiovascular events.

Objective To explore the effect of shRNA silenced aromatic hydrocarbon receptor (AHR) on WNT signaling path-way during the differentiation of P19 cells into cardiac myocytes. Methods The eukaryotic expression vector of mouse AHR gene was designed and constructed. The interference plasmid was transfected into P19 cell and the positive stains to AHR gene silencing were screened by G418. The mRNA expression of important genes GSK3βandβ-catenin were evaluated by real-time fluorescent quantita-tive PCR during the differentiation of P19 cells. Results The constructed AHR-shRNA plasmid significantly inhibited the expression of AHR gene. Along with the differentiation of P19 cell into cardiac myocytes, in the interference group the expression ofβ-catenin gene was lower whereas the expression of GSK3βgene was elevated than those of control group with significant differences (all P<0.01). Conclusions The interference of AHR gene expression can regulate WNT signaling pathway in the development of heart.

OBJECTIVETo investigate diagnostic efficacy of transvaginal three-dimensional hysterosalpingo-contrast sonography (3D-HyCoSy) in assessing tubal patency with chromolaporoscopy.

METHODSA total of 157 infertile women underwent 3D-HyCoSy to evaluate tubal patency. Among these patients, 39 patients were also examined by chromolaporoscopy. The concordance of the two clinical assessment methods was analyzed by the Kappa coefficient test.

RESULTSAmong the 306 oviducts examined by 3D-HyCoSy, 99 (32.4%) were patent, 126 (41.2%) partially obstructed, and 81 (26.5%) completely obstructed. Diagnostic results with 3D-HyCoSy were not statistically different from those obtained in the 39 women (78 oviducts) who also underwent chromolaporoscopy, and the two methods showed a high concordance (k=0.747, P=0.000). The 3D-HyCoSy procedure had a sensitivity of 84.8% (28/33), a specificity of 96.2% (25/26), and positive and negative predictive values of 93.3% (28/30) and 86.2% (25/29) respectively.

CONCLUSIONTransvaginal 3D-HyCoSy can accurately reveal the spatial path and morphology of the oviduct and is a safe and effective method to evaluate tubal patency.

Contrast Media ; Fallopian Tube Patency Tests ; methods ; Fallopian Tubes ; diagnostic imaging ; Female ; Humans ; Hysterosalpingography ; Imaging, Three-Dimensional ; Infertility, Female ; diagnostic imaging ; Laparoscopy ; Ultrasonography

Objective To study the characteristics of its pathologic and neurophysiologic changes of a ani-mal model of cervical spinal cord compression, and to explore the relationship between the severity of spinal cord inju-ry and its functions. Methods Thirty-two rabbits were divided into 4 groups randomly. A catheter was inserted intothe vertebral canal and a balloon was inflated to compress spinal cord in epidural space. According to the diameter ofballoon, the animals were divided into 4 groups (A, B, C, D group), Each group consisted of 8 rabbits. The corti-cal somatosensory evoked potential (CSEP) and motor evoked potential (MEP) were used to assess neurologicalfunction. Motor function behavior was scored before and on 1, 3, 7 and 14 days after surgery. Histological observa-tions were performed, pathological changes were observed by light and electron microscope. Results Spinal cordcompression resulted in a gradual increase of the peak latency and significant decrease of the peak amplitude. The la-tency and amplitude of MEP were changed more dramatically than those of CSEP. The result indicated that MEP wasmore sensitive than CSEP to the compression. Analysis also revealed that the severer the pathologic changes, the lon-ger the latency and the lower the amplitude of the evoked potentials. Conclusion The animal model presented inthis paper was simple and standardized. Evoked potentials as a noninvasive technique have great value in monitoringspinal cord function. The variance of amplitude and the latency in significantly correlate with the degree of compres-sion of the spinal cord.

Objective To investigate the expression of chemokine VCC-1 in hepatocellular carcinoma (HCC).Methods RT-PCR was used to detect the VCC-1 mRNA expressions in 8 HCC cell lines,10 normal liver tissues and 42 HCC tissues.Result In HCC cell lines,the expressions of VCC1 mRNA were high in SUN 398,intermediate in SUN387,SUN449,SUN423,HepG2,PLC5,and low in Hep3B and Huh7.In HCC tissues,the expressions of VCC-1 mRNA could be found in tumor and adjacent tissues.In these 42 tissues,VCC-1 mRNA was highly expressed in 26 specimens of tumor tissues (61％,14.9±7.6 fold) and 16 specimens of adjacent tissues (39％,6.9±5.4 fold).VCC-1 mRNA was up regulated in tumor tissues (P＜0.01).The expression levels of VCC-1 mRNA in 2 specimens were related to tumor differentiation and tumor size (P＜0.05).In the 10 specimens of normal liver tissues,no expression was detected in 8 specimens and light expression in 2 specimens.The expression was lower compared with cell lines,cancer tissues and adjacent tissues (P＜0.01).In 3 cases of recurrence,VCC-1 was highly expressed in cancer tissues (20.1±2.3 fold).In 8 specimenswith tumor thrombosis,5 tissues showed highly expressed VCC-1 (17.3±4.5 fold) while 3 specimens showed low expression.Conclusion VCC-1 plays an important role in HCC,and it may be considered as a potential therapeutic target of HCC.

Objective To investigate the tracing value of drug-loaded nano-active carbon for lymphatic visualization and its potential application in the lymphadenectomy of gastric cancer. Methods Sixty-three patients with advanced gastric carcinoma were randomized into 2 groups:control group and tracing group. For the tracing group, 5-Fu-loaded nano-active carbon (NP-5-FU) suspension was intratumorally administered by preoperative gastroscope-guided or intra-operative injection. No tracing reagents was given in the control group. The numbers of removed lymph nodes and metastasis-involved lymph nodes as well as the lymph node staining were recorded and compared between the two groups. The adverse effects was monitored. Results The resected lymph nodes in the tracing group were much more than that of control group (43.73 ± 9. 25 vs. 25. 36 ± 4. 73, t = 3.82,P ＜ 0. 05). For the N1, N2 and N3 lymph nodes, 27. 17 ± 5. 82,13.73 ± 4. 61 and 2. 83 ± 0. 15 per case of the tracing group were desected, while they were 14. 45 ± 2. 75,8. 27 ± 1.89, and 2. 64 ± 0. 11 per case in the control group,respectively (P ＜ 0. 05 ). 26. 2% (211/842) of the lymph nodes in the tracing group showed black intensity,higher than that of unstain lymph nodes (4. 5% ,21/470,χ2 =95. 108,P ＜0. 05) and that in control group (16.9%, 142/837)( χ2 =7.485 ,P ＜ 0.05, ). No local and systemic adverse reactions were observed for the local injection of nano-active carbon. No extension of operating time and increased complications occurred. Conclusion Local injection of nano-active carbon which was loaded with 5-FU around the tumor is a very useful measure to trace the lymph nodes and facilitate the lymphedetectomy and chemotherapy.