1.Content Determination of Oleanolic Acid in Radix Araliae Armatae by HPLC
Yi MENG ; Yixiong ZHONG ; Weifeng FAN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(09):-
Objective To establish a HPLC method for determining the content of oleanolic acid in Radix araliae armatae.Methods Stationary phase was Lichrospher-C18(4.6 mm?250 mm,5 ?m),mobile phase was methanol-0.2% phosphoric(85:15),detection wavelength was 210 nm,flow rate was 1.0 mL/min and column temperature was 30 ℃.Results There was good linearity in the range of 0.059~0.59 ?g for oleanolic acid.The average recovery of oleanolic acid were 95.4% with RSD=0.67%(n=6).Conclusion This method is rapid and accurate.It can be applied to control the quality of Radix araliae armatae effectively.
2.Determination of Emodin in Radix et Caulis Ventilaginis Leiocarpae by HPLC
Weian HUANG ; Yi MENG ; Weifeng FAN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(10):-
Objective To establish a HPLC method for determining emodin in Radix et Caulis Ventilaginis leiocarpae. Methods Stationary phase was Agilent Eclipse XDB-C18 (4.6 mm?150 mm, 5 ?m), mobile phase was methanol-0.1%phosphoric (75∶25), detection wavelength was 285 nm, flow rate was 1.0 mL/min and column temperature was 30 ℃. Results There was good linearity in the range of 0.006 3~0.063 ?g for emodin (r=0.999 9). The average recovery of emodin was 102.0% with RSD=1.58%. Conclusion This method is rapid and accurate. It can be applied to control the quality of Radix et Caulis Ventilaginis leiocarpae.
3.Effects of Body-weight Support Treadmill Training on Ambulatory Capacity of Individuals Following Incomplete Spinal Cord Injury
Xianguo MENG ; Xiaohua FAN ; Yi GONG ; Jiansheng DIAO
Chinese Journal of Rehabilitation Theory and Practice 2009;15(8):714-716
Objective To explore the effect of body-weight support treadmill training (BWSTT)on ambulatory capacity in individuals following incomplete spinal cord injury (ISCI).Methods 36 cases of incomplete SCI patients were divided randomly into control and BWSTT groups who were classified as ASIA C or D.All received comprehensive rehabilitation, and patients in BWSTT group also received BWSTT for 3 months when the physical condition was permitted.They were assessed before and after treatment.The evaluation items included measurement of ASIA lower-extremity motor assessment, functional independence measure (FIM) ambulatory function measure, 10meter ambulatory velocity and modified Barthel Index.Results The scores of ASIA lower-extremity motor assessment, FIM ambulatory function, the ambulatory 10-meter velocity in BWSTT group improved significantly when compared with the control group (P<0.05).The scores of modified Barthel Index were no difference between two groups before and after treatment (P<0.05).Conclusion BWSTT could improve ambulatory capacity for incomplete SCI patients
4.Research on Quality Evaluation Method of Geo-authentic Medicinal Herb Rhizoma Coptidis for the Reflection of Outstanding Feature of TCM
Gang FAN ; Ce TANG ; Jing ZHANG ; Xianrong LAI ; Xianli MENG ; Yi ZHANG ; Xinjian FAN
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(6):1274-1280
This study took geo-authentic medicinal material Rhizoma Coptidis as an example and analyzed soil characteristics of the growing environment of Rhizoma Coptidis . The methods of ISSR, HPLC, 1H-NMR and NIR were respectively used for exploring its genotype and investigating on its chemitype . In the respects of an-ti-endotoxin, anti-bacterial activities and insulin resistance that was related to the treatment of diabetes, the pharmacodynamic type was studied and the relationship was comprehensively analyzed . The quality evaluation of geo-authentic medicinal materials was preliminarily established based on ecotype , genotype chemitype and phar-macodynamic type of Rhizoma Coptidis . Simultaneously, quality standards of Rhizoma Coptidis were set up in combination with genuine character , which provided the scientific basis for establishing Chinese medicine char-acteristic methods of quality evaluation of Rhizoma Coptidis .
5.Prominent smooth muscle differentiation in fibroadenoma of breast: report of a case.
Jiong SHI ; Hong-yan WU ; Yi-fen ZHANG ; Fan-qing MENG ; Xiang-shan FAN
Chinese Journal of Pathology 2011;40(9):636-637
Actins
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metabolism
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Adult
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Calcium-Binding Proteins
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metabolism
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Cell Differentiation
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Desmin
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metabolism
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Diagnosis, Differential
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Female
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Fibroadenoma
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metabolism
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pathology
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surgery
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Hamartoma
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pathology
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Humans
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Hyperplasia
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Leiomyoma
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pathology
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Microfilament Proteins
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metabolism
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Muscle, Smooth
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pathology
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Phyllodes Tumor
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pathology
6.Stable interference on P210(bcr/abl) gene expression by lentiviral vector-delivered shRNA in vitro and in vivo.
Yu-Feng ZHU ; Yuan-Zhan WANG ; Fan-Yi MENG
Journal of Experimental Hematology 2012;20(5):1090-1094
P210(bcr/abl) fusion gene is indispensable for generation and progression of chronic myeloid leukemia (CML). Small molecule inhibitors, such as imatinib, are effective for P210(bcr/abl) gene mediated CML, but drug resistance may occur. The unique fusion junction of P210(bcr/abl) gene is an attractive target for therapeutic intervention using RNA interference (RNAi). This study was purposed to constructed the BaF3 cell line by viral vector which can stably express P210(bcr/abl) shRNA and P210(bcr/abl) mRNA at the same time, and investigate the effect of lentiviral-victor-delivered shRNA on P210(bcr/abl) gene expression. The infective rate of lentiviral vector on BaF3 cells with P210(bcr/abl) gene was assayed by fluorescent microscopy; the cell proliferation ability was determined by trypan blue exclusion; the P210(bcr/abl) mRNA and protein expressions were detected by RT-PCR and Western blot respectively. The results found that stable expression of the P210(bcr/abl) shRNA resulted in obvious inhibition of P210(bcr/abl) mRNA and protein expression and increased sensitivity of these P210(bcr/abl) gene transformed Ba/F3 cells to imatinib. The IC(50) to imatinib in these cells decreased < 50% as compared with Ba/F3-P210(bcr/abl) cells which did not express P210(bcr/abl) mRNA. The survival time of the lethal dose irradiated mice induced by intravenous injection of these Ba/F3 cells was longer than the other group induced by Ba/F3-P210(bcr/abl). It is concluded that stable expression of shRNA targeting the P210(bcr/abl) gene fusion junction may potentiate the effects of conventional therapy for CML.
Animals
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Fusion Proteins, bcr-abl
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genetics
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metabolism
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Gene Expression
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Genetic Vectors
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Lentivirus
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genetics
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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genetics
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metabolism
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Mice
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NIH 3T3 Cells
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RNA, Small Interfering
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genetics
7.Research progress and application of BCR/ABL transgenic animal model.
Yu-Feng ZHU ; Yuan-Zhan WANG ; Fan-Yi MENG
Journal of Experimental Hematology 2011;19(6):1532-1535
Transgenic animal model provide a good platform to research the pathogenesis and therapy of chronic myelogenous leukemia (CML). To date, a number of BCR/ABL transgenic animal models have been established using different promoter or tetracycline-controlling system. Some of them appear the characteristics of human CML, which have contributed greatly to research the pathogenesis and therapy of CML. In this article, the researching progress, advantage and drawback, application of BCR/ABL transgenic animal model are reviewed.
Animals
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Animals, Genetically Modified
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Disease Models, Animal
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Fusion Proteins, bcr-abl
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genetics
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
8.Analysis on formula raw materials application of health food containing Gardeniae fructus.
Yi-guang ZHANG ; Shi-huan TANG ; Qiang JIA ; Fan-yun MENG
China Journal of Chinese Materia Medica 2014;39(22):4470-4474
In this article we built formula database of health food containing Gardeniae Fructus with Traditional Chinese Medicine Inheritance Support System (V2.0). And on this basis, use data mining method such as association rules of the software, to analyze commonly used formula raw materials or materials combination of formula containing Gardeniae Fructus and raw material application having assisted function formula to protect chemical liver injury. The result shows that of the 71 health food formulas containing Gardeniae Fructus, most used materials are Gardeniae Fructus, Lycii Fructus, Angelica Sinensis Radix, Poria and so on. Commonly used materials combination mostly are Gardeniae Fructus and Lycii Fructus, Gardeniae Fructus and Angelica Sinensis Radix, Gardeniae Fructus and Poria, Gardeniae Fructus and Paeonia. There are nearly 18 healthcare functions of the health food containing Gardeniae Fructus, and most of these are assisted functions to protect chemical liver injury, and then immune modulating function. Of 23 formulas containing Gardeniae Fructus having assisted function formula to protect chemical liver injury, Gardeniae Fructus usually combined with traditional Chinese medicine which nourishs blood and liver such as Pueraria, Lycii Fructus, Hawthorn, Paeonia and Turnjujube. Analyzing formula raw materials application of health food containing Gardeniae Fructus contributes a lot to the further development and utilization.
Chemical and Drug Induced Liver Injury
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drug therapy
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Data Mining
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methods
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Food, Organic
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Fruit
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chemistry
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Gardenia
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chemistry
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Medicine, Chinese Traditional
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methods
9.Short-term follow up of bipolar arthroplasties for aged cases with intertrochanteric fractures
Wen-Xue JIANG ; Mao-Zhong HU ; Jia YOU ; Yi-Jin LI ; Meng FAN ;
Chinese Journal of Trauma 2003;0(11):-
Objective To evaluate the short term effect of bipolar arthroplasties in aged cases with intertrochanteric fractures.Methods There were 23 males and 15 females(at age of 70-93 years, average 76 years)with unstable intertrochanteric fractures treated with the third generation cementing techniques and bipolar arthroplasties that were followed up for average 2.4 years.The clinical effect was evaluate with Harris scale,X-ray films and complications.Results Of all,34 cases(89.5%)could walk freely.The average Harris scale was 84.2 points.The average period until walk with full-weight load was 5.6 weeks.The greater trochanters was united in 35 cases(92%)during average 4.2 months.No peri-prothetic ossteolysis and loosening or subsidence occurred.Conclusions Cemented bipolar arthro- plasty has good advantages of reduced laying up period and few complications.The short-term outcome is satisfactory hut the long-term outcome needs deeper observation.
10.Molecular mechanism of imatinib-induced thrombocytopenia in treatment of patients with CML.
Ling-Ling SHU ; Qian-Li JIANG ; Fan-Yi MENG ; Mo YANG
Journal of Experimental Hematology 2011;19(5):1314-1318
Imatinib mesylate has been commonly used in the treatment of patients with chronic myeloid leukemia (CML). However, a significant number of CML patients treated with imatinib developed thrombocytopenia. Platelet-derived growth factor (PDGF)/platelet-derived growth factor receptor (PDGFR) plays a significant role in the regulation of thrombopoiesis. It is suggested that imatinib may block the PDGF/PDGFR and PI3-K/Akt pathway, then inducing the apoptosis of megakaryocytes and developing thrombocytopenia in these patients. In this review, the potential molecular mechanism of imatinib-induced thrombocytopenia in the treatment of CML patients is discussed, including imatinib and thrombocytopenia, PDGF/PDGFR and thrombopoiesis, potential mechanism of imatinib-induced thrombocytopenia in treatment of patients with CML and so on.
Antineoplastic Agents
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adverse effects
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therapeutic use
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Benzamides
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Caspase 3
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metabolism
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Humans
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Imatinib Mesylate
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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drug therapy
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genetics
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metabolism
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Piperazines
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adverse effects
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therapeutic use
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Platelet-Derived Growth Factor
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metabolism
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Proto-Oncogene Proteins c-akt
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metabolism
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Pyrimidines
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adverse effects
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therapeutic use
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Signal Transduction
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Thrombocytopenia
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chemically induced
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Thrombopoiesis