Objective： To study topographic characters of the expression of Cx43 and the proliferation activity of keratinocytes on the upper legs of rats and to explore the relationship beween them. Methods： By rutine paraffin section , immunohistochemical techniques of Cx43 and PCNA were used to study the length of the expression of Cx43 in a unit area of epidermis(LA) and the percentage of proliferating cell nuclear antigen（PCNA）positive nucleus(%). Results： Within the range of 5 mm in the horizonal direction, the expression of Cx43 in epidermis varied according to the locations of keratinocytes and Cx43 was highly expressed which was about 3.0～4.5 mm from the start point. At the same time, within the range of 10 mm in the horizonal direction, the proliferation activity of keratinocytes was different from site to site and the kerationcytes with similar proliferation activity were clustered as groups, the scale of which was about 10－3 m; One of the sites, with low proliferation activity, was about 3.0～4.0 mm from the start point. Conclusion： This study suggested that the keratinocytes with low proliferation activity had high expression of Cx43.

ObjectiveTo explore the roles of Toll-like receptor (TLR) 3,7 and 9 in the pathogenesis of pityriasis rosea.MethodsThis study recruited 40 patients with pityriasis rosea and 40 normal human controls.Reverse transcription-PCR was carried out to detect the expressions of TLR3,7 and 9 in peripheral blood lymphocytes of these subjects.ResultsThe mRNA expressions of TLR3,7 and 9 in peripheral blood lymphocytes were significantly higher in patients with pityriasis rosea than in the normal human controls (Z =4.239,3.745,2.658,all P ＜ 0.05).There was a positive correlation between the expression of TLR3 and TLR7(r =0.574,P ＜ 0.01 ) as well as between that of TLR3 and TLR9 (r =0.551,P ＜ 0.01 ) in the patients,while there was no correlation between TLR7 and TLR9 expression in the patients or between the expressions of TLR3,TLR7 and TLR9 in the controls.ConclusionVirus infection might be involved in the pathogenesis of pityriasis rosea via TLR pathways.

Objective Measure the cytokines including hIFN-?、hIL-12 and hTNF-? which secreted by PBMC stimulated with recombinant BCG, and research how recombinant BCG influence the expression of cytokines and improve the immunologic response. Methods In the experiment, Recombinant hIFN-?-2b-BCG group and wild-type BCG group stimulate peripheral blood monocytes(PBMC) with different density in vitro, which is 0.1 OD and 0.01 OD (1 OD=2.7?107CFU). In third group, we put IFN-?-2b into wild-type BCG so that we can compare it with recombinant BCG. All these components foster with PBMC(4?106/ml), then collect the supernatant in 12 hours, 24 hours, 48hours, 72 hours, 5 day, 7day, and detect hIFN-?、hTNF-?, hIL-12 by an enzyme-linked immynosorbent assay(ELISA), compare the results. Results We can learn from the result, compared with the other groups, recombinant BCG can induce higher density cytokines than wild-type BCG’s and combination group’s(P

l014 faecal specimens were collected from 4 hospitals in Nanjing to investigate the infection of Cryptosporidium. Each faecal specimen was smeared and screened by auramine-phenol staining method for oocyst, the positive and suspected specimens were identified by modified acid-fast staining method and safranin-methylene blue staining method. The result showed that 13 spccimens were positive by auramine-phenol staining method and confirmed by modified acid-fast staining method. 5 suspected specimens were negative as shown by the latter two methods.The best result could be obtained when auramine-phenol staining method was first used and folloWed by modified acid-fast staining method. This-technique is simple, sensitive and reliable. It is difficult to find oocysts by safranin methylene blue staining method when the oocysts are few (Figs. l-3).

Sixty-eight lumbar intervertebral disc herniation (LDH) cases were treated with electroacupuncture on Jiaji points of the affected area with the following result after three courses of treatment: and 35 cases got recovery, 28 cases got better result, 5 cases had no effect, and the total effective rate was 92.6%.

Objective To observe the effect of local curettage combined with ointment in fistula applying in refractory apical fistula.Methods 56 cases of patients with refractory apical fistula one month after faultless root cannal therapy divided into group A and B by lottery,with 28 cases in each group.Local curettage combined with ointment in fistula applying in group A,and no processing applying in group B.One year fellow up was given to every patient to compare the effect between the two groups.Results All the 28 cases of patients with refractory apical fistu-la were recovering in three months.The improvement rate was 100%,recovery rate 100%,and the mean time of fistula disappearing was (0.5 ±0.1)months,time of shadow around root tip disappearing (2.5 ±0.7)months.Four cases in group B were improved in three months,three cases in six months and one case in nine months,with two cases recove-ring.The improvement rate was 28.57%,recovery rate was 7.14%,and the mean time of fistula disappearing was (5.0 ±0.7)months,time of shadow around root tip disappearing (9.4 ±1.0)months.The improvement rate in group A was higher than that in group B (χ2 =33.749,P<0.05),recovery rate in group A was higher than that in group B (χ2 =49.727,P<0.05).The mean time of fistula disappearing in group A was shorter than that in group B (t=3.081,P<0.05),mean time of shadow around root tip disappearing in group A was shorter than that in group B (t=5.042,P<0.05).Conclusion It was effective to apply local curettage combined with ointment in fistula applying in refractory apical fistula,and it is worthy of clinical popularization.

Neonatal sepsis is a systemic infection in which bacteria or fungi invade blood circulation and grow in it in the neonatal period,caused by toxin produce from bacteria or fungi.Currently sepsis is the major causing morbidity and mortality in neonatal infectious diseases,its clinical manifestation is not obvious and this brings us a range of clinical problems.In this review,we discussed these characteristics epidemiology,etiology,pathophysiology,clinical features of neonatal sepsis and septic shock in adults,to deepen the understanding of neonatal sepsis.

Chemotherapy resistance is a great obstacle for effective treatment of ovarian cancer.Drug resistance of ovarian cancer is the results of combined action of multi- factors.Further study of drug resistancerelated genes can provide new ideas for reversing drug resistance fundamentally.

Objective To study topographic characters of the proliferation activity of keratinocytes on the fore limbs of rats and to explore the relationship beween these characters and meridians. Methods By routine paraffin section,silver staining technique for NOR and immunohistochemical techniques of PCNA were used to study topographic characters of the proliferation activity of keratinocytes. Results The proliferation activity of keratinocytes on the fore limbs of rats is different according to the locations on the horizonal direction,and the keratinocytes with low proliferation activity are clustered into groups,the scale of which is about 10 -3 m.As result,3 4 ribbons of keratinocytes with low proliferation activity are formed on the vertical direction,where 3 4 meridians are supposed to be the positions of the comparative anatomy.Conclusion\ This study suggested that the keratinocytes on the meridians are characteritic of the low proliferation activity.\;[

Objective:To investigation for common hepatic cartery lymph node metastasis of esophageal squamous cell carcinoma.Methods:From 2015 to 2017, 413 patients with esophageal squamous cell carcinoma who were dissected the common hepatic cartery lymp node were admitted in Cancer Hospital of Sichuan. The relationship between metastatic of rates of common hepatic cartery lymp node and the clinical data including sex, age, tumor site, diameter of tumor, invasion depth, differentiation degree, pathological stage, neurovascular invasion status were reviewed retrospectively.Results:A total of 10 649 lymph nodes were dissected.The lymph node metastatic rate was 45.27%(187/413), and the metastatic lymph node ratio was 7.96%(848/10 649). Lymph nodes adjacent to the middle esophagus, cervical esophagus, cardia of stomach had a higher metastatic rate, while the Lymph nodes adjacent to the hilar, supraclavicular, common hepatic cartery had a lower metastatic rate.A total of 775 lymph nodes were dissected in common hepatic cartery.The lymph node metastatic rate was 7.02%(29/413), and the metastatic lymph node ratio was 4.51%(35/775). To analysis the relationship between metastatic of rates of common hepatic cartery lymp node and the clinical data.We conclusion that the common hepatic artery lymph node metastatic rates of upper, middle and lower esophageal squamous cell carcinoma were 2.89%(2/69), 5.35%(12/224) and 12.50%(15/120), with significant difference.The common hepatic artery lymph node metastatic rates of patients with diameter of tumor under 3 cm, 3-5 cm and above 5 cm were 5.20%(9/173), 5.52%(9/163)and 14.28%(11/77), with significant difference.The common hepatic artery lymph node metastatic rates of sex, age, tumor site, diameter of tumor, invasion depth, differentiation degree, pathological stage, neurovascular invasion status, with no significant difference.Conclusion:The metastatic rates of common hepatic artery lymph node in esophageal squamous cell carcinoma is lower. For the upper thoracic esophageal cancer with tumor diameter under 5 cm, the dissection of common hepatic lymph node can be ommitted in surgery.