Objective:To study the effect of comfort care to alleviate postoperative pain of tibial eminence fracture by fixation. Methods:55 patients with tibial eminence fracture between Apr 2013 and Mar 2015 in our hospital were selected and divided into observation group with 26 patients and control group with 29 cases according to the nursing methods. The control group was given the orthopaedic routine nursing method, and the observation group was given the comfortable nursing method to compare two groups of patients with postoperative pain and psychological status before and after the intervention (HAMA anxiety score and HAMD depression scale). Results:There were significant differences between two groups of patients with pain score after the intervention (t=9.545, P<0.05), and HAMA and HAMD scores decline. HAMA and HAMD scores in the observation group were significantly lower than that in the control group (t=11.593, t=10.628;P<0.05). Conclusion:Comfort care can significantly relieve postoperative pain of the tibial condyle spine fractures and improve postoperative anxiety and depression. It has good clinical application value.

Objective To disclose the inter-species differences among Papaver somniferum L, Papaver rhoeas L and Cannabis sativa L using AFLP analysis. MethodsThe root, stem, foliage, flower, seed of Papaver somniferum L, foliage of Papaver rhoeas L and Cannabis sativa L were collected respectively. DNA was isolated with AxyPrep Kit, and double-digested by EcoR I and Mse I, then oligonucleotide adapters were ligated. After Pre-amplification, selective amplification was performed using 6 pairs of fluorescent primer. The DNA fragments were analyzed using a CEQ 8000 DNA Fragment Analyzer. Results27 to 46, 5 to 20 and 4 to 31 pieces of amplified products were detected from Papaver somniferum L, Cannabis sativa L and Papaver rhoeas L respectively, and significant difference was observed among these three groups of products. The identical species-specific peaks were identified from root, stem, leaf, flower and seed DNA from the same Papaver somniferum L. ConclusionThe diacritical results from Papaver somniferum L, Papaver rhoeas L and Cannabis sativa L, as well as the identical results of different parts from the same plant demonstrated that AFLP analysis could be used possibly to determine the species origin of unknown plants samples.

Objective To observe the effect of acupuncture, moxibustion combined with bladder function training on the neurogenic bladder.Methods 64 patients were randomly divided into the treatment group and control group,treatment group was given acupuncture,moxibustion combined with bladder function training,while the control group was treated with simple bladder function training, then comparing the bladder function changes between two groups after a month.Results In the treatment group,the bladder capacity,residual urine volume,urinate rate were respectively ( 349.4 ±13.5 ) mL, ( 98.7 ±15.3 ) mL, 92.26% and the control group respectively ( 323.4 ± 17.8)mL,(127.8 ±17.8) mL,51.85%,the difference between the two groups were statistically significant ( t =5.48,5.60,χ2 =3.97,all P<0.05).Conclusion Acupuncture,moxibustion combined with bladder function train-ing can be effective in the treatment of neurogenic bladder,while improve the quality of patient's life.

BACKGROUND: It has been reported that magnesium sulfate(Mg2SO4)treatment has a satisfactory effect on brain ischemia-reperfusion injury, but its effect on spinal cord ischemic injury remains unclear.OBJECTIVE: To evaluate the effects of intravenous administration of Mg2SO4 on spinal cord ischemia-reperfusion injury and further probe into its mechanism.DESIGN: Randomized controlled repeatedly measuring design based on the experimental animals.SETTING: Central research laboratory of a university hospital.MATERIALS: The experiment was conducted in the Central Research Laboratory, Medical College of Xi' an Jiaotong University from April 2003 to June 2004. Twenty-seven New Zealand white rabbits with body mass of 1.9to 2. 5 kg were included. The rabbits were randomly divided into Mg2SO4group, normal saline group and sham-operation group with 9 rabbits in each group.METHODS: The lower segment of the kidney under abdominal aorta was occluded for 30 minutes and 48-hour reperfusion was performed to establish ischemia-reperfusion model of lumbosacral segment of spinal cord. Mg2SO4group (Group A, n = 9) received Mg2SO4 at the dose of 0. 25 mL/kg per hour throughout this procedure; the same volume of saline solution was used in normal saline group(Group B, n=9) . Animals of sham-operation group (Group C, n = 9) were anesthetized and received laparotomy without aortic occlusion. The somatosensory evoked potential(SEP) was detected before ischemia, 30 minutes after ischemia, and 1, 2, 8, 16 and 24 hours after reperfusion. Motor function score was assessed in Mg2SO4 and saline groups 24 and 48 hours after reperfusion. After reperfusion for 48 hours, the animals were killed and histopathological test was performed on the spinal cord.MAIN OUTCOM MEASURES: Motor function score, SEP monitoring and spinal histopathological test.RESULTS: The latency of SEP(Nt) was markedly longer 30 minutes after ischemia in Mg2SO4 group. It was obviously recovered during the first two hours after reperfusion compared with during ischemia, but was obviously prolonged after that. Waveform disappeared 30 minutes after ischemia in normal saline group. SEP amplitudes and latencies in sham-operation group did not change remarkably during the procedures and all the animals recovered without neurological deficits. At each reperfusion time point, the recovery of SEP(N1) latency was better in Group A than that in Group B( P ＜ 0.05). The average motor function score at 24 hours and 48 hours after reperfusion was significantly higher in Group A[ (3.7 ±0.5) and(3.4 ±0.7) points] than that in GroupB [(3.0±0.7) and (2.6±0.9) points](P ＜0.05). The normal nerve cell counting of spinal cord 48 hours after reperfusion in Mg2SO4 group(23. 4 ± 3. 4) was significantly higher than that in saline group (12.3 ±3.2)(P ＜ 0.01).CONCLUSION: Intravenous Mg2SO4 administration may reduce spinal cord injury and preserve neurological function in transient spinal cord ischemia in rabbits.

Adult ; Female ; Heart Neoplasms ; Humans ; Pericardium ; Pheochromocytoma

Objective To study the damage of rat articular cartilage induced by different doses of T-2 toxin, and to explore the relationship between mini-dose T-2 toxin and articular cartilage damage. Methods A total of 120 Wistar rats, weighing 50 - 70 g, were randomly divided into four groups according to their body weights: T-2 toxin group 0(control), 100, 200, 300 μg/kg, 30 rats in each group. Animals in the control group were fed standard rat chow, and animals in the three T-2 toxin groups were fed T-2-toxin-contaminated chow (the dose was 100, 200, 300 μg/kg, respectively). After 6 months, rats were euthanized by ether asphyxiation. The bilateral knee joints were collected and section prepared. The articular cartilage was examined by light and electronic microscope. Results Light microscope showed, the rat articular chondrocytes were clear and arranged orderliness in the control group. The rat articular chondrocytes were disarranged in 100 μg/kg T-2 toxin group.Degeneration and necrosis were found in 200 μg/kg group. Chondrocytes were shrunken with hypereosinophilia cytoplasm and fragmented pyknotic nuclei, extensive areas of chondrocyte loss and chondrocyte clones were visible in 300 μg/kg group. Scanning electronic micrograph(SEM) showed, the rat articular chondrocytes were clear, well formed and arranged tidy in the control group. The surface of articular cartilage was rough in 100 μg/kg group.Collagen fasciculi ruptured and stacked up in 200 μg/kg group. Presented a typical articular dryness phenomenon,the cartilage surface collapsed and many pits appeared in 300 μg/kg group. Transmission electronic microscope (TEM) showed that chondrocytes were abundant with cytoplasm, well-developed rough endoplasmic reticulum in the control group; agglomerate chromatin scattered along the karyotheca, nuclear membrane was thickening, with vacuolar degeneration of the endoplasmic reticulum in the 100 μg/kg group; endoplasmic reticulum expended, with protein retention and organelles breaks in the 200 μg/kg group. A large number of chondrocytes lost organdles, the membrane structures disrupted and the cartilage matrix stromatolyzed in the 300 μg/kg group. Conclusions Within the range of 100 - 300 μg/kg, T-2 toxin induces dose-related articular cartilage injury, the greater the dose, the more serious damage.

Diagnostics probation teaching has important value in the medical teaching for foreign students. The practice experiences of diagnostics probation teaching for foreign students in the last 3 years are reviewed from the management of teaching,practice of teaching,supervision of teaching and examine of teaching in order to improve the effect of diagnostics probation teaching for foreign students.

Objective To investigate the effect of AP-2α on the chemoresistance to oxaliplatin of colorectal carcinoma cell and its related mechanism.Methods Plasmid of GV102-AP-2α-RNAi (experimental group) and control plasmid GV102-NC (negative control group) were transfected into HCT-116 using Lipofectamine 2000 respectively.The AP-2α expression levels of mRNA and protein of experimental group,control group and HCT-116 blank group were detected by qRT-PCR and Western blot.Cell proliferation assay was performed using the CCK-8 and the apoptosis assays were preformed with Annexin V-PE Apoptosis Kit.Results The AP-2α expression levels of mRNA and protein both decreased after transfection of AP-2α-RNAi plasmid,moreover,the effect produced by subsequence 1 was the most significant.After treatment by oxaliplatin,AP-2α protein levels increased with time while mRNA did not change significantly.Western blot results suggested that the level of AP-2α protein in experimental group which was maintained in oxaliplatin was lower than the negative control group.CCK-8 results suggested that cell proliferation ability was significantly higher for the experimental group maintained in oxaliplatin [(88±3) ％] than the negative control group maintained in oxaliplatin [(57±3) ％] and the blank group maintained in oxaliplatin [(73t4) ％].Flow cytometry showed that the apoptosis rate of the experimental group maintained in oxaliplatin [(15.07±1.20) ％] was lower than the control group maintained in oxaliplatin [(24.93±0.90) ％] and the blank group maintained in oxaliplatin [(23.71±1.32) ％].Conclusion AP-2α may be related to the sensitivity of colon cancer cells to oxaliplatin.

Objective To investigate the present condition of unique copy of TCM ancient books, and analyze the literature value. Methods Bibliographic research was underwent first, then investigation table was made, investigation team for spot investigation was organized, the bibliography was sorted, and on this basis analysis of the literatures was performed. Results 59 collection units at home and abroad and 1370 unique copy TCM ancient books were investigated, which including 57 books published by photocopy and 37 books published by proof reading. Conclusion The unique copy TCM ancient books have important academic value and can be effectively saved and sorted through in-depth investigation and analysis.

Objective To evaluate the value of energy spectrum CT with multimodal quantitative parameters in diagnosis of the metastatic lymph nodes of gastric adenocarcinoma compared with the conventional CT.Methods 37 patients with gastric adenocarcinoma diagnosed by gastroscopy underwent enhanced CT scan with gemstone spectral imaging mode.We positioned and marked the stomach lymph nodes on CT images and compared with postoperative pathologic results one to one.ROC was used to identify the critical quantitative index of energy spectrum CT in distinguishing the metastasis from benign lymph node,and to evaluate the diagnostic effect compared with CT features.Results The 40-80 keV single energy spectrum curve slope (λHU )and normalized iodine concentration (NIC)in metastasis group were lower than those in the benign one in the arterial phase with statistical differences (P <0.05 ),but without statistical differences in venous phase.The combination of arterialλHU and NIC showed significant advantages compared with CT features(P <0.01), with the sensitivity,specificity and accuracy of 84.3% vs 61.4 %,88.4% vs 81.3 % and 86.8% vs 73.6 %.Conclusion The energy spectrum CT can provide quantitative analysis for differential diagnosis of gastric cancer metastasis from benign lymph nodes,and significantly improve the diagnostic accuracy of lymph node metastasis of the cancer.