Objective:To explore the perioperative treatment principle for arteriovenous fistulas of hemodialysis,the related factors to the success of operations and the intervention measures.Methods:1820 cases in the 2 periods from 1992to 2000 and from 2001 to 2008 were selected,and the relation of the change of primary diseases,the difference of their blood vessel condition before operation,different operation methods and different treatment after operation to the successful rate of operation and fistulas blood flow was analyzed and statistical analysis was made to the related data.Results:The ratio of secondary renal disease in the second period was much bigger and of significant difference with that in the first period(P

OBJECTIVE To survey the status quo of hospital infection management and countermeasures in township health center. METHODS To adopt a unified examination and assessment survey,the township hospital infection management,including organization,personnel training,monitoring,disinfection and isolation,occupational protection,sterile disposable medical supplies and medical waste management was investigated in 15 Lanxi Town ship Health Centers. RESULTS Only 2 centers had management organizations (13.33%),5 (33.33%) to participate in personnel/training,no one carry out the monitoring of hospital infection,with fuzzy concept about cleaning disinfection and sterilization and poor awareness of occupational protection. CONCLUSIONS Realizing a sound organization improving hospital infection management staff and intensive training,increaseing the investment and strengthening th supervision and inspection are in accordance with the law and guide management of patients in rural areas to be assured of a secure medical establishment.

Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous group of non-Hodgkin's lymphoma.An uncommon subset with myc and either bcl-2 or bcl-6 rearrangement,also known as ‘double-hit’ lymphomas,is considered very aggressive clinical course and poor prognosis despite high-intensity chemotherapy.Recently,these lymphomas have received increased attention.This review explores the existing literatures for the involved genes with their functions,clinical features,diagnosis and treatment.

Objective:In order to improve operator’s cognitive level for standardized disinfection, this paper will focus on analyzing and interpreting the disinfection procedures for some common dialysis machines.Methods: This paper will interpret and compare the disinfection procedures for three brands of dialysis machines in the following aspects: the choice of the disinfectant, the disinfection method, the concentration of the disinfectant and the injected volume of the disinfectant, etc.Results: The procedures of hot disinfection with citric acid were used completely in those three brands of dialysis machine, and the procedure of sodium hypochlorite need been used periodically to remove the proteins and other organic matter.Conclusion:Although the dialysis machines provide almost complete and efficient disinfection procedures, but the operator should need to select the most suitable procedures according to their practical situation. If the operators cannot master the disinfection procedures and have not noticed the importance of it, the wrong operator will lead to incomplete disinfection, affect the normal operation of the dialysis machines and increase the risk of cross-infection.

Objective To study the polysaccharides from dried rhizome of Ligusticum chuanxiong.Methods DEAE-Cellulose and gel-filtration chromatography were used to isolate and purify the polysaccharides,whose structures were characterized by chemical and spectral methods.Results Four homogeneous polysaccharides,named LCP-1,LCP-2,LCP-3,and LCP-4 were obtained from the water extract of L.chuanxiong.Four structures were investigated to be complicated polysaccharied fractions,with the molecular weights of 3.1?10~4,5.2?10~4,9.0?10~4,and 3.6?10~4,respectively,then estimated by GPC.Conclusion The four polysaccharides are obtained from this plant for the first time.

Objective Comparing the variability of four genes of HCMV in the population and the therapy process of single patient.Methods Using PCR to amplify the four genes and restriction enzyme to validate four fragments, These four genes are UL55,UL57,UL86 and UL122 whose coding products are glycoprotein B,single chain binding protein,main capsid protein and IE pp86 separately. Then we use AD169 to optimize the conditions of PCR and restriction enzyme. then we use these conditions to detect 200 blood specimens. Results The PCR positive rate of UL55 is 12.5% and restriction positive rate is 10.0%, which has marked difference with other 3 gene segments(P

The low sensibility of MR molecular imaging to signals makes signal amplification exceptionally important.This paper reviews the strategy of the reporter gene signal amplification in MR molecular imaging.

DNA methylation is an important epigenetic system. It plays many crucial roles in the gene regulation. With the development of the high-throughput detection techniques, the bioinformatics study has been an active hot topic in the research of DNA methylation. The major achievements and progress on the prediction of DNA methylation status, the mechanism that the majority of CpG islands are resistant to DNA methylation, the relationship between DNA methylation and other epigenetics, as well as the association between aberrant DNA methylation and the tumorigenesis were reviewed in this article.

Objective To investigate the expression of microRNA-451 (miR-451) in female invasive ductal carcinoma (IDC) and its roles in tumor cell invasion.Methods Forty five IDC tissues and matched tumor adjacent tissues were collected between January and December in 2014.The expressions of miR-451 in tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR).The relationship between long noncoding RNA taurine regulated genes 1 (lncRNA-TUG1) and clinical features was analyzed by student-t test.miR-451 mimics was transfected into MDA-MB-231 cells.Transwell assay was used to measure cell invasion ability.The expression of c-myc,a potential target of miR-451,and its downstream genes,matrix metalloproteinase (MMP)-2 and MMP-9 were detected by qRT-PCR and Western-blot.Resuts The expression of miR-451 was significantly lower in IDC tissues than in matched tumor adjacent tissues (P ＜ 0.05).Low expression of miR-451 was positively associated with lymphatic metastasis (P ＜ 0.05) and advanced tumor node metastasis (TNM) stage (P ＜ 0.05).Up-regulation of miR-451 in MDA-MB-231 cells could significantly suppress cell invasion ability (P ＜0.05).c-myc expression was down-regulatedwhen miR-451 was transfected (P ＜0.05).As downstream genes of c-myc,the expressions of MMP-2 and MMP-9 were suppressed.Conclusions miR-451 is down-regulated in IDC tissues and associated with cell invasion.miR-451 might inhibit IDC progression by inhibiting c-myc/MMP axis.

Objective To detect EB virus in peripheral blood of patients with non-hodgkin lymphoma (NHL) by ramification amplification method (RAM).To investigate the relativity of NHL with infection of EB virus.Methods RAM was used to detect EB virus with the man-made target gene sequence of promotor of EBER-1. In 120 cases of patients with NHL, EB virus were detected by RAM, and the result of RAM was compared with that of PCR.Raji cells and NB4 cells were used as positive control and negative control respectively.Results RAM could detect 10 molecular target gene of the exponent.76 cases were positive in 120 cases of NHL patients and the positive rate was 63.3%, The result is identical with that of PCR.Conclusion Ramification amplification method is sensitive, convenient and easy to perform.NHL has a high relativity with infection caused by EB virus.