OBJECTIVE:To study the combined antibacterial activity of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae against Helicobacter pylori(Hp).METHODS:The protocol was designed by checkboard method.The MICs of Rhizoma Coptidis,Bee Propolispro and Fructuts Evodiae alone or in two-and-two combination against Hp were determined by agar plate method,and the FIC indexes was calculated for the evaluation of the combined effects.RESULTS:When Rhizoma Coptidis combined with Bee Propolispro,the FIC index was 0.16(0.5)when combined with Fructuts Evodiae;The FIC index was 0.05(

OBJECTIVE:To select the best medium prescription of HP.METHOD:To select the best solid medium of HP by orthogonal test design and to study the condition of preserving HP.RESULTS & CONCLUSIONS:There are significant deviations of growing circumstance of HP on the different cultivation medium.The best solid cultivation medium for HP is colombian agar or brain heart infusion agar medium with 2.5%mixed antibiotic added with 5.0%bovine serum and kept the pH of medium at 7.5.HP is preserved under —70 ℃～—80 ℃,which could be successfully antibiotic.

OBJECTIVE: To construct hospital pharmaceutical website and facilitate the evolvement of hospital pharmaceutical care.METHODS: Website management system was developed independently and the system was used to construct hospital pharmaceutical website in our hospital.RESULTS & CONCLUSIONS: Website management system which was designed with B/S three-tier structure which sustains multi-level management authority control and a variety of database and runs stably.The professional website based on website management system is convininent and maintenance,rapid in operation,cheap in cost,low in consumption of human resouces,comprehensive in function,highly professional,and it has greated enhanced the working efficiency in hospital pharmacy.

AIM: To investigate the relationship between Alzheimer disease(AD) and alpha 2-macroglobulin(α2M) initially so as to provide evidence for the pathogenesis of late-onset AD.METHODS: The significance of α2M in the late-onset AD was revealed through pathology of α2M in AD and the polymorphism of α2M in AD patients.RESULTS: The association between AD and α2M region on the twelfth chromatosome was analyzed with the genic linkage, and pathological symptom of α2M in AD patients was supported by many evidences. Two polymorphism sites of α2M were located on the 12P12-13, which could increase more risks to AD patients.CONCLUSION: It is significant to investigate the frequency, mutation,polymorphism and expression of gene α2M for the pathogenesis of late-onset AD and its genic diagnosis.

Magnetic resonance imaging (MRI)has apparent dominance in nasopharyngeal carcinoma (NPC) diagnosis,staging,the plan of radiotherapy target and assessment of efficacy because of its high-contrast of parenchyma and muli-parameters imaging.Especially diffusion-weighted imaging (DWI) developed in several years,it can provide qualitative and quantitative information at the cellular level about tumor cellularity and the integrity of cell membranes,which is valuable for tumor detection,cervical lymph nodes metastases,systemic transfer,monitoring and predicting the treatment response of nasopharyngeal carcinoma.

The reaction product of cerium used for. localization of 5'-nuclcotidase (5'- Nase) and p-nitrophenylphosphatase(p-NPPase) is highly electron-dense but lacks sufficient contrast at the light microscopic level. We describe two methods for converting the reaction product of cerium to colored compounds visible by light microscopy. Using the Ce-Pb method and Ce-Pb-AgS method, the reaction prod- ucts of 5'-Nase and p-NPPase in isolated neurons of frontal cortex of rabbit were visualized by light microscopy. Aftcr application of the Ce-Pb-AgS method, surface of isolated neurons exhibtied crystalline precipitates by scanning electron microscopy(SEM). X-ray microanalysis of these crystals revealed the presence of high amounts of Ce, S/Pb and Ag. These results demonstrate that enzyme activity could be verified by SEM in conjunction with elementary microprobe analysis.

AIM:To observe the clinical effect of Yinxingdamo Injection (extract of Folium ginkgo, Dipyridamole) in the treatment of cerebral infarction. METHODS: 170 cases with acute cerebral infaction were grouped at random. The treatment group (120 cases) was given 20mL of Yinxingdamo Inection in 500mL of 5% Glucose Injection by IV infusion, once a day for 14 days. The control group (50 cases) was given 20mL Compound Danshen Injection in 500mL of 5% Glucose Injection by IV infusion, also once a day for 14 days. RESULTS: The total effective rate of the treatment group was 82.5%. It was higher than that of the control group (62%) ( P

l014 faecal specimens were collected from 4 hospitals in Nanjing to investigate the infection of Cryptosporidium. Each faecal specimen was smeared and screened by auramine-phenol staining method for oocyst, the positive and suspected specimens were identified by modified acid-fast staining method and safranin-methylene blue staining method. The result showed that 13 spccimens were positive by auramine-phenol staining method and confirmed by modified acid-fast staining method. 5 suspected specimens were negative as shown by the latter two methods.The best result could be obtained when auramine-phenol staining method was first used and folloWed by modified acid-fast staining method. This-technique is simple, sensitive and reliable. It is difficult to find oocysts by safranin methylene blue staining method when the oocysts are few (Figs. l-3).

Non-cirrhotic portal hypertension(NCPH)is a group of diseases that show evidences of portal hypertension but no cirrhosis is present.Common causes of NCPH include pre-sinusoidal portal lesions such as portal vein thrombosis,congenital liver fibrosis and idiopathic portal hypertension,and post-sinusoidal portal lesions.The major feature of this group of diseases is well preserved liver function in spite of prominent portal hypertensive manifestations such as esophageal varices/gastrointestinal bleeding and splenomegaly/hypersplenism.Careful differentiation from cirrhosis requires thorough clinical,radiological and pathological investigation.Preventing and control of variceal bleeding and hypersplenism through medical,endoscopic and interventional procedures yield good prognosis in most of the patients with NCPH.

After the implementation of health education for schistosomiasis control among floating population in Cixi City, Zhejiang Province, the awareness rate of knowledge on schistosomiasis control and correct behavior rate of floating population increased from 33.5% and 34.5% to 90.1% and 93. 2% , respectively, and the initiative examined rate of floating population increased obviously.