The existing literature on fingerprint chromatography at home and abroad and the quality control of biochemical injection with multiple components were reviewed in this article.Combined with the laboratory research, It is proposed that the strategy for HPLC specific chromatography of biochemical injection with multiple components in order to provide the basis for effectively promoting the establishment and development of HPLC specific chromatography of biochemical injection with multiple components.

The aim of this study was to obtain the alginate gels which could have proper compressive strength and excellent permeability for cell proliferation and could have more promising potentials in the application of tissue engineering. Through the reaction of the carboxyl of the alginate and the amino of methacrylic acid, methylacrylic was generated into alginate long chain which could be enhanced by the polymerization of double bond under thermal reaction condition. And then alginate gel beads were prepared using the mixture of calcium chloride and barium chloride solution as cationic crosslinker, and the compressive modulus and permeability of the prepared alginate gel beads were investigated. When the ratio of barium ions to calcium ions was 5:5, the compression modulus was 189.7 kPa, and it showed the best permeability for trypsin with molecular weight of 24 kDa and entrapment effect for bovine serum albumin with molecular weight of 67 kDa. Compared to compositions of other ratios, the alginate gel beads made in 5:5 mixture indicated excellent compressive modulus and permeability. These results indicated that the alginate hydrogel beads with the ratio of barium ions to calcium ions being 5:5 have a potential application in tissue engineering as a support material and encapsulating materials in cell culture.
Alginates ; chemistry ; Barium Compounds ; chemistry ; Calcium Chloride ; chemistry ; Cations ; Cell Culture Techniques ; Cell Proliferation ; Chlorides ; chemistry ; Cross-Linking Reagents ; chemistry ; Gels ; chemistry ; Metals ; Polymethacrylic Acids ; chemistry ; Tissue Engineering

Objective To analyze the ultra microstructure and histological properties of implanted composites of decalcified bone matrix (DBM) impregnated calcium phosphate cement (CPC) with recombinant human bone morphogenetic protein-2 (rhBMP-2) in bone defect, and evaluate the osteoplastic efficiency of this composites. Methods The rabbit DBM was prepared beforehand. The composites of DBM impregnated CPC with rhBMP-2 was made at 0.2 proportion of DBM. The rabbit's bone defect of femur condyle was filled with implantation of the composites (group A, n=12) or CPC (group B, n=12) or bone cement (group C, n=12). Animals were sacrificed at 6, 12, or 24 weeks after operation, and the implants were examined by histological technique and scanning electron microscopy (SEM). Results In group A, at the 6th week after operation, the interface region between implants and bone tissue became fuzziness, and was crossed by the generated fibers of bone tissue, and filled with woven bone growing inside into the composites; at the 12th week after operation, blood vessels, osteoblasts and new bone were generated inside of composites; at the 24th week after operation, the bone defects were recovered and became solid union, most of the implants were replaced by new bones. In group B, at the 6th week after operation, the interface of CPC and bone tissue was sharp, and was not crossed by new bones; at the 24th week after operation, the bone defects were still filled with materials of CPC and no new bone was found inside CPC. In group C, at the 24th week after operation, the interface of bone cement and bone remained non-union. Higher osteoblastic activity,more neogenetic blood vessels and higher growth rate of woven bone were observed in group A compared with those in group B. Conclusions For bone defect, the implantation of composites with DBM proportion of 0.2 can stimulate the growth of osteoblast, blood vessel and woven bone. It is biodegradable and can be replaced by autogenous bone.

Objective: To examine the effects of recombinant human growth hormone on the acute phase response in abdominal sepsis patients.Methods: Prospective unblinded trial was made with each patient serving as his/her own control.Eighteen abdominal sepsis patients were enrolled ranging in age from 24 to 60 yrs.All patients received 10 U/d of GH 48 hrs after performing drainage and for 7days.Results: Serum concentration of constitutive proteins,acute phase protein and cytokines were measured before and 1,3,5 and 7 days after GH administration.GH notablely increased serum constitutive protein levels(prealbumin,transferrin,fibronectin) and decreased serum acute phase protein level(CRP).Cytokine TNF-?,IL-1? and IL-6 had significant change after GH administration,but the changes were not relative to the change of acute phase response.Conclusion: Low-dose rhGH effectively modulated the acute phase response by attenuating production of the hepatic acute phase protein and increasing the synthesis of constitutive proteins.

The microRNA let-7 family,functioning as a tumor suppressor,has emerged as a regulator of stem cell differentiation and tumor repression.The RNA-binding protein lin28 is a stem cell pluripotency factor,which contributes to the maintenance of stem cell characteristics and promoting cell malignant transformation.lin28 can block the maturity of let-7 by combining with the precursors of let-7 family miRNAs,which is itself able to repress lin28 by binding to the 3'-UTR of lin28 transcripts,thus forming a feedback loop.The double-negative feedback loop comprising lin28 and let-7 is involved in several biological processes such as differentiation of stem cell,tumorigenesis,invasion and metastasis,drug resistance and relapse,which couldbe a potential target for cancer therapy.

Objectives:To observe the efficacy of enteral nutrition with short peptide elemental diet(Pepti 2000) in patients with gastrointestinal fistulas and evaluate the sensitivity of nutrition associated index in nutrition monitoring. Methods:Ten cases with gastrointestinal fistulas were administered total enteral nutrition(TEN).They received 146 kJ/(kg?d) of non protein calorie(NPC) and 0.25 g/(kg?d) of nitrogen per day. Body weight,serum albumin, transferrin(TFN), prealbumin(PA),fibronectin(FN),triglyceride and cholesterol were monitored on before and 7th and 28th after TEN. Results:Seven days after TEN,serum PA and FN levels rised significantly( P

Objective To study the effect of methyl mercury on the development of rat offspring. Methods The male Wistar rats were divided into four groups randomly, each group was administrated methyl mercury by gavage every two days for two months, then, the rats were mated with healthy female rats. The development, growth and behaviors of offspring were investigated. Results Compared with the control, the body weights of offspring in groups of 1.170 mg/kg and 0.580 mg/kg were less and the development was tardy. The indexes that can reflect the level of development showed that the behaviors of offspring in experimental groups were damaged. Conclusion The male rats exposed to methyl mercury may produce an adverse effect on the development, growth and behaviors of their offspring.

Objective:To study the inhibitory role of BsAb(anti-human bladder cancer/anti-VEGF) on human bladder cancer growth.Methods:BsAb was used to treat animal models of BIU-87 cell lines.The tumor inhibition rate was calculated and the tumor microvessel density was detected by immunohistochemical techniques.Results:BsAb could significantly inhibit growth of tumor.And the tumor microvessel density was lower than that of the control group.Conclusion:BsAb could inhibit tumor growth by inhibiting angiogenesis. BsAb may become a new method for human bladder cancer treatment.

Objective To seek an effective method for the treatment of grade ⅢB tibial fractures.Method From Oct. 1985 to Oct.1999, 26 cases of grade ⅢB tibial fractures were treated by early soft tissue coverage(including 12 cases of free muscle flaps and 14 cases of local muscle flaps), early fracture fixation(including 21 cases of external fixation and 5 cases of non-reamed intramedullary nail) and early bone grafting(15 cases).Results The follow up duration were from 10 to 32 months with an average of 15.5 months. The success rates of free and local muscle flaps were respectively 91.7 %(11/12) and 92.9 %(13/14)?Infection occurred in 5 cases (3 cases of local superficial infection and 2 cases of osteomyelitis). The time for bone union were from 17 to 66 weeks with an average of 42 weeks. Conclusion "San-Zao"therapy,especially early soft tissue coverage and early fracture fixation is on effective management for grade ⅢB tibial fractures with the advantages of promoting wound healing and bone union and decreasing the possibility of infection.

Objective To investigate the association between diabetes mellitus and risks of kidney cancer.Methods The cohort and case-control studies on the association between diabetes mellitus and risk of kidney cancer were analyzed by searching PubMed database within the time limit of Jan.1990 to Oct.2011.Data were selected according to the inclusion and exclusion criteria,and the meta-analysis was performed by using the Cochrane Collaboration's RevMan 5.0 software.Results A total of 14 studies(8 cohort studies and 6 case-control studies) involving 6,645,516 participants met the inclusion criteria.Meta analysis showed that patients with diabetes had a higher risk of kidney cancer compared with the control group without diabetes(OR =1.28,95％ CI 1.10 ～ 1.48,P ＜ 0.05).Stratification study showed that diabetes was associated with an increased risk of kidney cancer in cohort studies(OR =1.32,95％ CI 1.14-1.53,P ＜0.05),but this association was not found in casecontrol studies (OR =1.21,95 ％ CI 0.79-1.84,P ＞ 0.05).Conclusion This meta-analysis indicates a positive association between diabetes and risk of kidney cancer,however,future researches are needed to identify whether diabetes mellitus is an independent risk factor for kidney cancer.