Objective To investigate the relationship of serum uric acid(SUA) with type 2 diabetes mellitus and diabetic macrovascular complications and to study the influential factors of SUA.Methods Recruited were eighty type 2 diabetic patients(group B),ninety-two type 2 diabetic patients with coronary artery disease(group C) and one hundred and twelve healthy controls(group A) whose qualitative urine protein assay were negative.Serum concentrations of uric acid and other biochemical materials were assayed at a fasting state.The sensitivity of insulin was also evaluated.Results(1) The SUA levels in group A,B and C were(280±81)(257±76) and (302±72) mmol/L respectively;SUA in group B was lower than that in group A(P=0.037) or group C(P=0.000) and SUA in group C tended to be higher than that in group A(P=0.050).(2) SUA level significantly increased in man than in woman(P=0.001).(3) The independent influential factors of SUA were WHR(R=0.354) in man,and BMI(R=0.286) and age(R=0.623) in woman.(4) After adjusting the age,sex,distribution of body fat and serum lipid level, the logistic regression analysis of group B and C showed that hyperuricemia was the independent risk factor for diabetic macrovascular complications,and this was especially obvious in smoker or hypertensive patient.Conclusions SUA in type 2 diabetic patients is lower than that in both healthy controls and type 2 diabetic patients with coronary disease.The level and contributing factors of SUA are different between men and women.

Objective To observe and study the application and effect of antimicrobial agents in type Ⅰ general surgery, in order to rationalize the use of drugs.Methods 228 patients with type Ⅰgeneral surgery from May 2014 to September 2016 were selected as the observation group.The patients in the type Ⅰ general surgery group from February 2012 to April 2014 were selected as the control group.The observation group were retrospectively monitored, and the control group were prospectively monitored.The application of antimicrobial agents in the two groups of patients, the rational application and the application rate of antimicrobial agents in type Ⅰ general surgery and outcome were compared.Results The application rate of the first generation cephalosporins in the observation group was significantly higher than that in the control group ( P<0.05 ).There was no significant difference in application rate of macrolides compared with the control group.The application rates of the second generation and third generation cephalosporins, quinolones, aminoglycosides and nitroimidazoles were lower than those of the control group ( P<0.05 ).And the rational use of antimicrobial agents, reasonable choice of time, rational combination of drugs, reasonable dose, reasonable frequency of administration and reasonable volume of solvent were higher than those of the control group, the differences were statistically significant (P<0.05).The antibiotic application rates of abdominal hernia surgery, thyroid surgery and breast surgery were lower than those of the control group, the differences were statistically significant (P<0.05).There was no infective case in the two groups.And the average length of stay in the observation group was (8.50 ±1.20) days, which was lower than (15.00 ±2.30) days in the control group, the difference was statistically significant (P<0.05).Conclusion Accordance with the principles of antibiotics in type Ⅰ general surgery, it could rationalize medication and reduce the drug resistance.

OBJECTIVE: To study on the effect of different extracting methods on the content determination of polysaccharide from Caulis Dendrobii.METHODS: Polysaccharide was extracted from Caulis Dendrobii respectively by reflux extraction,improved reflux extraction,ultrasound wave extraction and ultrasonic-assisted thermal reflux extraction.The content of polysaccharide was determined by phenol-sulfuric acid method.RESULTS: The determined results using these methods were as follows:(13.473?0.634)%,(15.692?0.743)%,(11.87?0.536)% and (16.29?0.407)%.CONCLUSION: The content determination of polysaccharide from Caulis Dendrobii was associated with the extraction method.The polysaccharide from Caulis Dendrobii could be extracted more thoroughly and rapidly by ultrasonic-assisted extraction technology.Therefore this method could be used to pretreat the sample in content determination of Caulis Dendrobili polysaccharide.

The design principles of healthcare wearable devices: ambient intelligence, service continuity, and micro-context provide more choice and solutions for the healthcare and social needs, and have the potential to be an integral part of the modern health care system. The use of wearable devices will contribute to the innovation of healthcare data acquisition and healthcare behaviors, the promotion of health consciousness and literacy, it also benefits the patient education, clinical pathway, medical model and health management performance.

Female ; Hepatitis C ; complications ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interferons ; therapeutic use ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; drug therapy ; virology ; Middle Aged ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Treatment Outcome

GRP78,as endoplasmic reticulum resident chaperone,is highly induced by a variety of tumor microenvironmental stresses,such as hypoxia and glucose deprivation.GRP78 is implicated in tumor cell proliferation,apoptosis,invasion,metastasis and angiogenesis.Recent evidence indicates that GRP78 levels is correlated with pathological grade,stage and prognosis for the majority of solid tumors.In addition,GRP78 plays an important role in drug tolerance and knockdown of GRP78 has been shown to sensitize malignant cells.GRP78 could not only be a good biomarker to predict cancer progression and chemo-responsiveness,but also an appealing target for the development of a more selective chemotherapy.

Objective To monitor lung growth rule of normal middle-late pregnancy fetal. Methods 599 cases of 18 and 40 weeks normal singletons were grouped by every two weeks. The length of right lung in the sagittal plane and the cross-sectional plane were measured, and the growth curve was described. The ratio of right lung area (RLa) and head circumference (Hc),chest circumference (Tc) and of the chest area (Ta) in different gestational ages was calcalated. The correlation coefficient was obtained by applying statistical analysis. Results There was a linear relationship between RLa/Hc and RLa/Tc and growth of gestational age;the regression equation was:(RLa/Hc: ?Y= 0.948X-1.221, r2 =0.898, the RLa/Tc: ?Y=0.944X-1.800, r2 =0.894). RLa/Ta in different ges-tational age was in constant range:0.42~0.55. The length of right lung in the sagittal plane and the cross-sectional plane increased along with the gestational age, and the growth speed was:before and after diameter(the cross-sec-tional plane) > upper and lower diameter (sagittal plane) > about size (the cross-sectional plane). Conclusion Prenatal ultrasound can evaluate the growth rule of normal fetal lung and provide the basis for assessment of fetal lung maturity.

Objective　To establish the method of 2-dimensional electrophoresis(2-DE) for proteins of human spermatozoa and to construct a protein map of human spermatozoa. Methods　The sperm pellet was prepared with simple Percoll layer protocol. We studied the effects of various sample preparation methods, loading quantities and isoelectric-focusing protocols on the quality of silver-stained 2-DE map, and constructed a primary protein map of human spermatozoa. Result　Up to 703 protein spots were acquired with sample preparation Method Ⅰwhile only 194～210 spots with Method Ⅱ.With immobilized pH gradients and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(IPG-DALT) we could acquire over 700 spots while only 280～300 with isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis(ISO-DALT). Conclusion　It is satisfactory to lyse sperm with sample preparation Method Ⅰ and to separate sperm proteins by IPG-DALT for establishing 2-D map of human sperm.

Objective To explore relativity between serum cortisol and post-stroke depression in old patients with stroke. Methods Serum cortisols of 41 stroke patients were examined two weeks after stroke, and they were all assessed through Hamilton Depression Scale and then happening rates of post-stroke depression observed in both normal cortisol group and enhanced cortisol group were tested through χ2 test. Meanwhile relativity between serum cortisol level and post-stroke depression were analysised. Results Happening rates of post-stroke depression had an evidently difference between both normal cortisol group and enhanced cortisol group(χ2=4.11;P <0.05). Mark of normal cortisol group was 15.29±7.28, enhanced cortisol group was 25.85±10.81, both groups had an evidently difference( P <0.01). Serum cortisols were not obviously relative to depression degree in normal cortisol group( r =0.295;P =0.194) while they were obviously relative in enhanced cortisol group( r =0.702;P =0.001). Conclusion Enhanced cortisol could forecast higher possibility of depression after the old post-stroke, and detecting cortisol could offer theory foundation for prevention and cure of post-stroke depression.

Acupuncture Therapy ; Adult ; Bloodletting ; Breast Diseases ; therapy ; Combined Modality Therapy ; Female ; Humans ; Hyperplasia ; therapy ; Mammary Glands, Human ; physiopathology ; Middle Aged ; Young Adult