Objective:To detect and analyze the membrane estrogen receptor(mER)in primary cultured interstitial cells of Cajal(ICC).Methods:Interstitial Cells of Cajal(ICC)'s surface binding sites for 17-?estradiol(E2)were detected by cell-impermeant ligand using confocal microscopy.Radioligand binding assay and Scatchard software were used to analyzed the characteristics of mER.Results:Immunofluorescence shows the staining pattern of nonfixed,nonpermeabilized ICC incubated with E2BSAFITC.The radioligand binding assay were analyzed by Scatchard software,The Bmax of mER was 45.75 fmol/mg protein and the kD was 0.717 3 nmol/L.Conclusion:A form of the estrogen receptor is present within the cell membrane of ICC and maybe capable of mediating rapid effect of estrogen.

Objective To investigate the influence of trimetazidine combined with atorvastatin on heart func-tion and the BNP level in patients with cornary heart failure.Methods The clinical data of 400 patients with cornary heart disease were retrospectively analyzed.194 patients in the control group were treated with conventional treatment, 206 patients in the observation group were treated with trimetazidine combined with atorvastatin on the basis of the control group.The treatment effect,cardiac function and BNP level were observed and compared.Results The effec-tive rate of the observation group was 91.26%,which was significantly higer than 76.29% of the control group (χ2 =16.667,P <0.05).After treatment,the cardiac index,stroke volume,cardiac output,left ventricular ejection fraction and BNP level of the two groups were significantly higher than before treatment,and those in the observation group were significantly higher than the control group(t =2.505,2.851,3.308,3.452,all P <0.05).Conclusion Trime-tazidine combined with atorvastatin in treatment of cornary heart failure has good curative effect,it can reduce the serum level of BNP,improve cardiac function significantly,which is of great value for clinical use.

Objective To explore the distribution and characterization of class 1 integrons in E.coli from healthy feces,and to elucidate the status of gene-cassettes.Methods Routine method was used to isolate E.coli,antibiotics susceptibility was tested by the disk diffusion method;class 1 integron was detected by PCR assay;PCR products were sequenced and analyzed.Results Of 97 samples,76 isolates were identified,and 25 isolates were multiple-drug resistant.The antibiogram was sulfamethoxazole-trimethoprim,ampicillin,streptomycin,tetracycline,erythromycin.14 of 25 isolates carried class 1 integrons,and the size of integrons differed from 1 800 bp(10 strains) to 750 bp(4 strains).The sequenced PCR product demonstrated that the 1 800 bp integron laboured aadA1-dfrA14-orf gene cassette conferred the resistance to sulfamethoxazole-timethoprim,streptomycin and aminoglycoside;the 750 bp integron laboured dfrA14 gene cassette conferred the resistance to sulfamethoxazole-trimethoprim.Conclusion The different kinds of class 1 integrons exist in E.coli from the healthy students,and determine the multiple-resistant antibiotics.

Owing to the advanced age,limb hemiplegia,dehydration,and vessel wall injury,stroke may be easy to cause venous thromboembolism (VTE).VTE mainly include deep vein thrombosis (DVT) and pulmonary embolism (PE).DVT refers to abnormal blood clotting in the veins and impedes venous return.The dislodgement of emboli from the vessel wall can form a thrombotic embolism,and cause PE,myocardial infarction,and stroke; it can not only prolong hospitalization,but also increase the mortality.This article reviews the incidence,risk factors,treatment,and prevention of DVT after a stroke.

Objective To analyze the causes of misdiagnesis and mistreatment of traumatic abdominal hernia. Methods In this study,11 cases of traumatic abdominal hernia admitted during 1971 to 2004 were retrospectively analyzed.Hernia located subcutaneously in 8 cases including incarcerated hernia in 2 csses.Hernia located intermuscularly in 2 cases with one case being incarcerated.and the other strangulated.One located retroperitoneally.Among the 11 cases,3 were simple hernia,8 were complicated with injuries of other organs. Results Eight cases were misdiagnosed,5 were operated on with wrong incision,and in one case the herniated intestinal loop,mistaken as hematoma,was incised.All these 5 cases underwent a second surgery due to failure in repairing the hernia. Condusion For traumatic abdominal wall hernia,correct diagnosis,the correct timing of operation and an appropriate incision chosen insure good therapeutic results.

A total of 100 in-hospital patients with acute stroke were randomly assigned to receive traditional Chinese medicine Radix et Rhizoma Rhei combined with abdominal massage(once daily; study group, n=50) or observation (control group, n=50 ) to prevent from constipation. After 3 days' treatment, 3 patients (6%) in the study group and 44 patients (88%) in the control group developed constipation (χ2=67.482, P<0.01). In addition, no adverse effects occurred in the study group. Our results indicated that traditional Chinese medicine Radix et Rhizoma Rhei combined with abdominal massage might help acute stroke patients to prevent from constipation, achieve earlier health recovery, and improve quality of life.

A probe into the management of outpatient medical records written by rural doctors:Such a record is indispensible for technical appraisal of medical accidents; yet writing of such a record might involve illegal medical practice or go beyond the scope of practice.It is recommended to normalize the behavior of rural doctors in their writing of outpatient medical records,stipulating that the Basic Regulations on Medical Records do not apply to rural doctors at present.It is also recommended that separate regulations for rural doctors be formulated,along with a promotional period before these regulations are in practice.An alternative would be clarifying that Article 28 of the Regulations of Medical Malpractice Settlement do not apply to rural doctors.

Objective To investigate the relationship between absent end-diastolic velocity (AEDV) in umbilical artery and perinatal outcome,and to explore the effect of termination time of pregnancy on perinatal outcome. Methods Data of thirty five pregnant women with AEDV admitted into Beijing Obstetrics and Gynecology Hospital from January 2008 to December 2009 were retrospectively analyzed.According to gestational age and treatment,they were divided into four groups.Group 1:AEDV was identified before 28 weeks without treatment (n=5); Group 2:AEDV was found before 28 weeks,and then was treated (n=13); Group 3:AEDV was found after 28 weeks,and was not treated (n=11); Group 4:AEDV was found after 28 weeks,and then was treated (n=6). Except for three patients in Group 2,all patients had complications.Data were presented by frequency,rate or mean±SD. Results Among the 35 patients,19 (54.3％) delivered and 23 living children were born.When AEDV was found,the gestational age was less than 28 weeks and the mean gestational age was (22.8 ± 2.2) weeks in Group 1,(24.2 ± 2.0) weeks in Group 2,however,the gestational age was over 28 weeks and the mean value was (30.9± 2.8) weeks in Group 3 and (29.5±0.8) weeks in Group 4.Treatment was given to women in Groups 2 and 4,but not in Group 1 and 3.In Group 1,one patient complicated with twin-twin transfusion syndrome; one with hemolysis,elevated liver enzymes and low platelet syndrome and placental abruption; three recovered after two weeks and babies survived.In Group 2,the termination time was (31.4 ± 5.5)gestational weeks and the duration of treatment was (10.7± 5.5) days.AEDV of six patients were not improved after treatment,among which five accepted induced abortion,one had preterm delivery.The rest six women underwent cesarean section and one term delivered.Babies survived except for one preterm neonate and one lost in follow-up.In Group 3,the termination time was (31.2 ± 2.9)gestational weeks.Five patients accepted artificial abortion for severe complications of mother or babies; the other six patients accepted cesarean section,among which one complicated with placental abruption and baby died.The rest five babies survived.In Group 4,the pregnancies were terminated at an average of (32.8±2.9) gestational weeks and the duration of treatment was (10.8±6.7) days.Two accepted induction after treatment and four recovered to normal after treatment and accepted cesarean section with four wellbeing babies. Conclusions Perinatal outcomes of patients with AEDV complicated with severe complications after active treatment might relate to the termination time.Some patients with AEDV without severe complications might recover spontaneously with good prognosis.

Objective To compare the influence of two different managements of relief incision (exposed relaxation therapy or absorbable hemostatic gauze) in infants' palatoplasty. Methods A total of 118 infants with cleft palate between 5- to 8-month-old for the study were randomly divided into two groups: group A was exposed therapy group, and in group B both sides of relief incision were placed absorbable hemostatic gauze. The wound bleeding, body temperature, diet and incidence of fistula were observed and analyzed after operation. Results There was no significant difference between the two groups in postoperative temperature, diet and wound healing (P>0. 05). The postoperative wound bleeding was statistically significant differences between the two groups (P<0. 05). Conclusions Either exposed therapy or placed absorbable hemostatic gauze in relief incision, has minimal systemic and local reactions in the postoperative infant. The advantages of exposed treatment are economic and simple, and less irritant to the wound.

This study was aimed to reveal the effector mechanisms of Chinese medicine aconite, ginseng, astragalus and dried ginger on the intervention of adriamycin (ADR) cardiotoxicity model rats. The analysis was made on the interactive relationship between aconite and ginseng, astragalus as well as dried ginger. A total of 72 rats were randomly divided into nine groups. There were eight rats in each group. Except the normal group, rats in each group were intraperitoneally injected with 2.5 mg·kg-1 of ADR according to their body weights. The injection was given once a week and continued for four weeks. The total dosage was 10 mg·kg-1. In the aconite, dried ginger group, the intragastric administration dosage of herbal decoction was 1.75 g·kg-1. The decoction dosage in the gin-seng, astragalus group was 0.875 g·kg-1. The decoction dosage in the Shenfu, Qifu group was 2.625 g·kg-1. The decoction dosage in the Jiangfu group was 3.5 g·kg-1. The intragastric administration was given once a day and continued for four weeks. Indexes such as superoxide dismutase (SOD), cardiac troponins (cTn), cytochrome C (CytC), myocardial mitochondria of Bax, Bcl 2, caspase-3, caspase-9 were detected. The colligation score was calculated associating with the close index. One-way ANOVA was given on different indexes and colligation indi-cators among different drug groups and the factorial design variance analysis was given to reveal the drug interac-tions. The results showed that compared with the normal group there were statistical significances among different indexes in the model group (P < 0.05). Aconite, ginseng, astragalus, dried ginger had varying degrees of impact on different indicators. There were statistical significances on the interaction between aconite and ginseng, astra-galus, dried ginger (except Bax). It was concluded that herbal medicine aconite, ginseng, astragalus, dried ginger had certain protective effect to the heart of ADR model rats. The combination of aconite and ginseng, astragalus, dried ginger can enhance the effect compared with a single herb.