OBJECTIVE: To establish an HPLC method for the determination of the content of chlorogenic acid in Ergan oral liquid.METHODS: The sample was determined on Scienhome C18(250 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-0.4% phosphoric acid(12∶88) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 327 nm.RESULTS: The linear range of chlorogenic acid was 0.169 6～0.848 ?g(r=0.999 9) and the average recovery rate of chlorogenic acid was 98.88%(RSD=0.48%,n=6).CONCLUSION: The method is simple,accurate and reproducible,and it is applicable for the quality control of Ergan oral liquid.

Objective To assess the effect of cryopreservation of cornea in glycerol,to analyze the protective effect of sodium hyaluronate on corneal endothelial cells(CEC) during preservation,and to find out a convenient and cheap method for the storage of cornea.Methods 20 rabbit corneas were divided into two groups randomly.The corneas of control group were stored directly in pure glycerol at-25℃,while in the experimental group sodium hyaluronate was evenly anointed on the corneal endothelial surface of the cornea before being cryopreserved in glycerol.2 months later,the effect of preservation for both groups was examined with trypan blue combined with alizarin red staining(viability staining) for corneal endothelial cells,electron-microscope examination for preserved corneas,and rabbit experimental penetrating keratoplasty(PKP) with the long-term cryopreserved corneas from both the experimental and control groups.Results CEC viability could be preserved by both methods.CEC trypan blue combined with alizarin red staining(viability staining) showed that the CEC density of cornea in the experimental group was 3 067.9?127.6/mm2,which was higher than that of control group(2 899.9?134.0/mm2).The mortality of CEC in experimental group was 4.2%?3.4%,lower than that in control group(13.8%?2.2%).Suceessful rate of PKP was higher in the experimental group with higher CEC density.Conclusion Cryopreservation with glycerol can preserve the viability of CEC.Sodium hyaluronate can reduce the damage to endothelium in the preserving procedure and can obviously improve the effect of preservation.The improved cryopreservation of cornea with glycerol may be a convenient,economic and effective method for eyebank.

AIM: To establish the quality standard for Haimo Blood-Stanching Tablets(cuttle fish sepia) by means of quantitative and qualitative experiments. METHODS: Melanin was identified by physics and chemistry reaction.Gly,Asp,Glu and Ala in Haimo Blood-Stanching Tablets were determined by HPLC-AccQ-Tag method and iron was determined by atomic absorption spectrophotometer. RESULTS: Melanin in sepia could be dissolved in 2 mol/L NaOH and there was something black separated out when in 2 mol/L HCl.The recoveries of four kinds of(amino) acids and iron were all in the range of 95%-105%.Each RSD was below 5%(n=6). CONCLUSION: The quantitative and qualitative analysis methods are accurate and reproducible.This study provides a method for the quality control of Haimo Blood-Stanching Tablets.

In recent years, the development of molecular biology and medicine has prompted the research of gene therapy for brain diseases. In this review, we summarized the current gene therapy approaches of major brain diseases. Against the pathogenesis of major brain diseases, including brain tumors, Parkinson's disease, Alzheimer's disease and cerebrovascular disorders, there are several effective gene therapy strategies. It is no doubt that, gene therapy, as a novel treatment, is of great significance for understanding the causes, as well as comprehensive treatment for brain diseases.

Objective To investigate the feasibility, safety and efficacy of the stent-assisted coil embolization for the treatment of wide-necked anterior communicating artery (ACoA) aneurysms. Methods From November 2011 to November 2014, the clinical data of the patients with wide-necked ACoA aneurysms treated by using the stent-assisted coil embolization were analyzed retrospectively. The clinical and radiographic effects were summarized. The perioperative complications were focused on. Results The ACoA wide-neck aneurysms of 29 patients were treated with stent-assisted coil embolization. Two of them abandoned the interventional treatment because of stents could not be in place and release. The immediate results of interventional treatment showed that 21 were embolized completely (78% ), 5 were near totaly embolized (19% ), and 1 was partialy embolized (3% ). Three patients had intraoperative thrombosis (recanalization achieved after thrombosis, one of them died of cerebral hemorrhage after thrombolysis). One patient suffered cerebral angiospasm, one had aneurysm rupture, and one had distal rupture of the anterior cerebral artery. Twenty-seven patients received clinical folow-up, and the folow-up time ranged from 2 to 36 months (mean 17 months). None of the patients had hemorrhagic or ischemic complications. Sixteen patients received imaging folow-up, and the folow-up ranged from 6 to 12 months (mean 7 months). None of the aneurysms had recurrence. Conclusions Stent-assisted coil embolization of wide-necked ACoA aneurysm is overal feasible, safe, and effective although there are certain risks.

This study was aimed to analyze the current prescription combination rules on traditional Chinese medicine (TCM) in gastric cancer treatment, in order to explore reasonable application of data mining technology in the study of prescription combination rules. Modern literatures were searched in CNKI and WanFang database. Frequency analysis, association analysis and composition network were used comprehensively. Rules such as herb application frequency and combination rules of the target prescription set were analyzed. The results showed that after screening and standardization, 116 prescriptions were included in the set which including 1 269 herbs and involving 17 types of 212 herbs. It was concluded that TCM paid attention to strengthen body resistance in gastric cancer treatment. It can also be combined with drugs for invigorating blood circulation and stasis, heat-clearing and detoxification, dispersing phlegm and stasis, as well as regulatingqi stagnation depending on the syndrome.

Child ; Child Welfare ; trends ; China ; Humans

Objective To study the correlation of renal function and plasma ammonia in patients with liver cirrhosis combined with hepatorenal syndrome .Methods The clinical data of 149 patients with liver cirrhosis who came to this hospital from February 2008 to April 2013 was collected .All the patients were divided into the HRS group (hepatorenal syndrome ,35 cases) and non-HRS group (non-hepatorenal syndrome ,114 cases) .The clinical datas and related biochemical indicators of two groups were analyzed and compared .Results The serum level of TB ,DB ,AST ,ALT ,BU ,CREA and NH3+ of the HRS group was statistically higher than that of the non-HRS group ,while the serum level of TP ,Alb ,NA and CL of the HRS group was statistically lower than that of the non-HRS group;results of Pearson′s analysis showed that the serum level of BU and CREA were positively correlated with NH3+ in HRS group .Conclusion The relationship between hepatorenal syndrome and plasma ammonia in patients with liver cir-rhosis is closely .Intestinal acidification can be used to reduce the risk of hepatorenal syndrome and hepatic encephalopathy .

Objective To investigate the intervention effect of Qingxin-Ⅱ Recipe on myocardial cell apoptosis and its fibrosis in viral myocarditis mice and its mechanism.Methods A total of 120 Balb/c mice were randomly divided into the model group (n=50),the treatment group (n=50) and the control group (n =20).Mice in the model and treatment groups were given intraperitoneal injection of CVB3 to establish the experimental model of viral myocarditis.The control and model groups were treated with normal saline and water by intragastric administration respectively and the treatment group was given Qingxin-Ⅱ Recipe.The expression level of nuclear factor-κB (NF-κB),tumor necrosis factor (TNF-α),interleukin-1β(IL-1β),collagen type Ⅰ-A1 (COL1-A1),collagen type Ⅲ-A1 (COL3-A1),matrix metalloproteinase-9 (MMP-9),tissue inhibitor of metallproteinase-1 (TIMP-1),myocardial interstitial collagen volume fraction (CVF) and apoptosis rate (Rapo) were assessed after treatment.Results The levels of NF-κB,TNF-α,IL-1 in cardiomyocytes,serum levels of COL1-A1,MMP-9,TIMP-1,the mRNA expression levels of COL1-A1,COL3-A1,MMP-9,TIMP-1 in cardiomyocytes,CVF and Rapo were lower in the treatment and control groups than in the model group (F=21.541,12.146,32.583,4.212,6.863,4.868,9.089,8.662,7.307,6.646,4.324,41.237,respectively,all P＜0.05).No significant differences in the levels of NF-κB,TNF-α,IL-1 of cardiomyocytes,in serum levels of COL1-A1,TIMP-1 and in mRNA expression levels of COL1-A1,COL3-A1,MMP-9,TIMP-1 of cardiomyocytes between the control group and the treatment group (all P＞0.05) were found,but serum MMP-9 level was higher in the treatment group than in the control group (P =0.033).Conclusions Qingxin-Ⅱ Recipe can effectively reduce inflammatory cytokines levels,protect myocardial cells from inflammatory damages and inhibit myocardial fibrosis in viral myocarditis.

Objective To introduce the method of treating chronic mallet finger using Micro Arc bone Anchor, and investigate the feasibility of this method with the clinical effects.MethodFourteen chronic mallet fingers were treated with Micro Arc bone Anchors. During the operation, a "?" skin incision over the dorsum of the DIP joint was made to expose the extensor tendon and resect the scar tissue between the disrupted ends. Then the DIP joint was made at extending position was immobilized with a 1mm Kirschner wire and the ends of extensor tendon was sutured with horizontal mattress suture method. After that,the Micro Arc bone Anchor was sutured into the dorsal base of the distal phalanx,the suture thread attached the end of anchor was tied to the proximal end of the extensor tendon to reinforce the former suture. External fixation on the anterior face of forearm was applied to immobilize the wrist at 30?of extention and the metacarpophalangeal and interphalangeal joints and extending position for 3 w, and Kirschner wire fixation was taken out 6 w postoperatively. Functional exercises were performed after the Kirschner wires were removed.ResultAll the cases were followed up for 6 months to 1 year, and excellent results were obtained in 10 cases, good in 2, fair in 2 and poor in 1 according to Dargan's functional assessment system, with dysfunction of flexion in DIP joint in 1 case, and recurrence of abnormity in 1 case.The total rate of excellent and good results was 85.7%.ConclusionMicro Arc bone Anchor is a convenient and effective alternative for the treatment of chronic mallet finger deformity with many advantages such as the high suturing intensity,low recurrence rate of abnormity, convenient for operation and reliable effect.