Objective To observe the effects of berberine hydrochloride on the secretion of interleukin-6(IL-6)from human periodontal ligament cells(PDLCs)cultured in vitro.Methods Periodontal ligament tissues were isolated from extracted human premolars,and PDLCs were cultured in vitro.After identification by immunohistochemistry technique,human PDLCs were divided into LPS group and NLPS group according to whether lipopolysaccharide(LPS,50 ?g/mL)was contained in cell culture media.Then in accordance with the final concentrations of berberine hydrochloride in cell culture media(0,0.010,0.020,and 0.030 g/L),the groups were subdivided into LPS and NLPS control group,LPS1 and NLPS1 group,LPS2 and NLPS2 group,LPS3 and NLPS3 group.IL-6 contents in supernatants of each group were determined by enzyme-linked immunosorbent assay,and statistical analysis and comparison were conducted as well.Results Compared with corresponding LPS and NLPS groups,IL-6 contents in supernatants of LPS3 group and NLPS3 group reduced markedly(P

Amyloid beta-Peptides ; analysis ; Animals ; Frontal Lobe ; drug effects ; physiology ; Hippocampus ; drug effects ; physiology ; Learning ; drug effects ; Memory ; drug effects ; Rats ; Rats, Wistar ; Sodium Azide ; pharmacology ; Space Perception

Animals ; Disease Models, Animal ; Facial Nerve Injuries ; etiology ; Female ; Male ; Rabbits

Concealed penis is usually found in children, which affects the patients both physiologically and psychologically. Some of the patients are wrongly treated by circumcision, which may bring about serious consequences to the sexual life of the patients in their adulthood. In the recent years, this disease has been receiving more and more attention from both doctors and parents. However, controversies remain as to its classification, pathogenesis, pathology, and treatment. This paper focuses on the understanding and advances in the studies of concealed penis.
Child ; Circumcision, Male ; adverse effects ; psychology ; Humans ; Male ; Penis ; abnormalities ; surgery ; Sexual Behavior

Ear Diseases ; congenital ; surgery ; therapy ; Ear, External ; abnormalities ; Ear, Middle ; abnormalities ; Humans

Nowadays more and more attention has been paid to the problems about housing and health in China. Some investigations showed that people spent almost fourteen hours in their homes every day so the houses become the major places of daily activities. It is very important to solve the problems of what is the relation between the housing and health and how the housing influences the health of the people living in it. In the present paper the author summarized the overseas and domestic researches on the housing and health and pointed out that the research on housing and health in China is just in the preliminary phase.

BACKGROUND: Hydroxyapatite-geltin composite has good biocompatibility and osteoinductivity, and can used be as tissue engineering scaffold to repair bone defects. OBJECTIVE: To observe the effect of three-dimensional (3D) printed hydroxyapatite/gelatin scaffold combined with bone marrow mesenchymal stem cells and umbilical vein endothelial cells in repairing rabbit skull defects. METHODS: Nine male New Zealand white rabbits were taken to establish a skull defect model with a diameter of approximately 0.8 cm, and randomly divided into three groups: blank group: no any treatment; control group: only 3D printed hydroxyapatite-geltin scaffold; experimental group: 3D printed hydroxyapatitegeltin scaffold and bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells complex group, with three rabbits in each group. At 8 weeks after the operation, CT scan of the skull pyramid beam and histological observation of the skull defect were performed on the white rabbits of each group. Animal experiments were approved by the Ethics Committee of Jining Medical College. RESULTS AND CONCLUSION: (1) CT scan of pyramidal tract: Blank group showed obvious bone defects, and the defect area was slightly radiopaque with the edges of the surrounding normal bone tissue. Control group showed some new bone formation, which was discontinuous and inconsistent with the surrounding bone tissue. Experimental group showed that the new bone tissue was linear and continuous; the thickness was thin; and the defect area merged with the adjacent bone tissue edge. (2) Histological observation: Hematoxylin-eosin staining and Masson trichrome staining showed that fibrous connective tissue formation and a small amount of free bone cells were seen in the defect area of the blank group. A small amount of bone formation was seen in the control group. Bone matrix was deposited at the edge of the material, replacing the material to form small bone trabeculae. The material space of the experimental group was gradually replaced by new bone, and the defect area was filled with new bone and trabecular bone structure-like tissue. (3) The results show that the 3D bionic printing hydroxyapatite-geltin scaffold combined with bone marrow mesenchymal stem cells and bone marrow mesenchymal stem cells can effectively promote the growth of bone tissue and accelerate the repair of bone defects.

Objective: To develop an expeditious method for the simultaneous determination of main active compounds in Fufang Ejiao Syrup (FES, compound Ejiao oral liquid) by near infrared spectroscopy (NIRS) in transflective mode. Methods: The contents of total flavonoids and total saponins were determined by colorimetric assays, while the soluble solid content was determined using a moisture analyzer as reference value. Partial least squares regression (PLSR) was employed for the calibration of regression models between NIRS and the indexes. Results: The correlation coefficients (R2) of the constructed models for total flavonoids, total saponins, and soluble solid content were 0.9798, 0.9660, and 0.9978, respectively, and the root mean square errors of prediction (RMSEP) were 69.6, 17.6 μg/mL, and 0.394%, respectively. Conclusion: The proposed method is fast and accurate, and facilitating the rapid analysis of main active components in FES.

Objective To evaluate haematogenesis of complex Shenlu granule in treating myelodysplastic syndrome (RA/RCMD) due to kidney yang deficiency. Methods The qualified cases were randomly divided into the complex Shenlu granule group and the Andriol group. During treatment, indexes such as peripheral hemogram, T cell subgroup, NK cell and immunological phenotypic expression of BMC were tested. Results Compared with prior treatment, Rbc, Hb and Plt have significantly increased in Shenlu group [prior treatment: (2.39±0.99)×1012/L, (84.47±28.68) g/L, (81.13±96.85) ×109/L; post-treatment (2.80±0.98)×1012/L, (94.87±25.63) g/L, (98.67±107.9)×109/L, and the difference was statistically significant (t =4.0359, P =0.001, t =2.7009, P =0.0172, t =2.2573, P = 0.0405). Andriol group has only increased Hb [prior treatment: (71.93±27.53) g/L; post-treatment (80.07±26.03) g/L] was statistically significant (t =2.3125,P =0.0365). After treatment, CD+4, CD+8, CD+4/CD+8, and NK were at or near normal value in Shenlu group (37.9±5.9) %, (24.0±5.8) %, 1.75±0.83, (13.0±6.9) %, compared with prior treatment (29.3±11.7) %, (29.6±5.8) %,1.12±0.59, (8.8±5.7) %. The difference was statistically significant (t =2.6194, P =0.0202, t = 2.6595, P =0.0187,t =2.6581, P =0.0187, t =2.2288, P =0.0427, P <0.05), and CD+8, CD+4/CD+8 close to normal in Andriol group [(22.1 ±7.5) %, 1.50±0.74], prior treatment [(26.6±7.5) %, 1.18±0.55]. The difference was statistically significant (t =2.2377, P =0.0420, t =2.9352, P =0.0109, P <0.05). After treatment, CD+4 [Shenlu group:(37.9±5.9) %, Andriol group: (30.5±12.6) %] difference was statistically significant (t =2.1738, P =0.0474) in two groups; Shenlu group control abnormal expression of bone marrow cells, which were CD+13, CD+33, CD+34, CD+64,CD+117. The effect was better than Andriol group (the first three: u =2.76, u =3.39, u =2.85, P <0.01, the latter two: u =2.17, u =2.46, P <0.05). Conclusion clomplex Shenlu granule can effectively control normal hematopoietic function for MDS (RA/RCMD) due to kidney Yang deficiency.

It provides a new approach to treat myelodysplastic syndromes when decitabine comes out.In order to improve the understanding of the role of decitabine and to provide a reference for the clinical treatment of middle-risk and high-risk myelodysplastic syndromes,this article mianly summarized drug combination,prognostic molecular markers,and reviewed the mechanism of action,main medication regimen in recent 5 years for the treatment of middle-risk and high-risk myelodysplastic syndromes with decitabine.