Helicobacter pylori is able to colonize the human stomach and dwell in the human stomach for decades or for whole lifetime. A number of potential virulence factors contribute to Helicobacter pylori to colonize this unusual niche. Motility is an essential colonization factor based on the fact that nonmotile variants of Helicobacter pylori can't infect gnotobiotic piglets. Motility is not a colonization factor based on rapid loss of motility of Helicobacter pylori in the gastric lumen in vivo. The exact roles of Helicobacter pylori motility are not yet known. The aim of this article is to discuss correlation between colonization and motility of Helicobacter pylori.

Humans ; Lysosomal Storage Diseases ; prevention & control ; therapy ; Lysosomes ; metabolism

Cervical carcinoma is a serious threat to the health of women around the world ,and its inci-dence ranks at the second position after breast cancer in female reproductive system .In addition to oncogene acti-vation and inactivation of tumor suppressor gene ,endogenous and exogenous factors also affect the development of cervical cancer .

China ; Humans ; Infant, Newborn ; Metabolism, Inborn Errors ; diagnosis ; therapy ; Neonatal Screening ; Phenylketonurias ; diagnosis ; Tandem Mass Spectrometry

Some studies corresponding modernization of Chinese materia m edica(CMM)were performed in the institute for the new technologies,materials,and facilities based on the achievements and experiences gotten in the past two dec ades.The technologies of large-scale propagation of plant cell,tissue,and organ were applied to the products of cell metabolism,biotransformation,and artificial breeding and rooting.Many new types of large-scale bioreactors were designed an d used not only for laboratory but also for manufactory.Reaction/separation inte gration technology,microwave-assisted extraction,and the other new extraction te chnologies were studied to realize high efficiency and low energy.Besides tradit ional separation and purification methods,new technologies,including extraction in reversed micelles,one step three-phase extraction on Penicillin,foam fraction ation,membrane separation,and high-speed counter-current chromatography(HSCC),we re developed.HSCCC is a kind of liquid-liquid partition chromatography without a ny solid matrix,which eliminates irreversible adsorption of samples on solid sup port in the conventional chromatographic column.It has been successfully applied to the analysis and separation of various natural products.At the same time,man y types of microsphere and microencapsules for controlled release and separation media were prepared and lots of attention was paid on drug modifying and embedd ing techniques.HSCCC was also applied as a new method to the study of CMM finger print and showed good precision and repeatability.High performance liquid chroma tography(HPLC),gas chromatography(GC),high performance capillary electrophoresis (HPCE),and mass spectrometer(MS)played a very important role in fingerprinting.C ontinuous propagation-continuous collection technological process was set for la rge-scale propagation of micro-algae in the study of marine drugs.Transgenic alg ae were cultured to prduce genetically engineered products.

Education, Nursing, Continuing ; General Surgery ; education ; Humans ; Physicians

Objective To optimize the method of primary culture for human amniotic epithelial cells (AECs) and detect the expression of hepatocelluar specific proteins in AECs. Methods AECs were obtained by collagenase and trypsin digestion method. 10,20,40 ng/mL epidermal growth factor (EGF) and 10 ng/mL basic fibroblast growth factor were added separately to the culture media,inverted microscope was used to observe the cell growth and proliferation,and the optimal condition for primary culture of AECs was obtained. The primary cells cultured without growth factors were served as controls. The expression of hepatocelluar specific proteins such as albumin,cytokeratin-18 (CK-18),alpha-1 antitrypsin (AAT) and alpha-1 foetoprotein (AFP) in cultured cells and histological sections were detected by immunohistochemical staining. Results The AECs available were relatively pure,with only a few mesenchymal cells. EGF of 10 ng/mL was chosen as an ingredient of culture medium as the growth and proliferation of AECs significantly accelerated with 10 ng/mL EGF. The expression of albumin,CK-18,AAT and AFP was detected in amnion tissues and AECs cultured in vitro. Conclusion Collagenase and trypsin digestion method and culture medium with 10 ng/mL EGF are favourable conditions for primary culture of AECs. Hepatocelluar specific proteins are expressed in human amnion tissues and AECs cultured in vitro,indicating that AECs have some characteristics of hepatocytes.

Root canal biofilm is frequently detected in the canal wall of infected root canal and the root canal with failed root canal therapy. Due to its special structure and diverse composition, root canal biofilm has the ability of the drug tolerance and antiimmunity, which lead to apical periodontitis. This review summarizes the features of the root canal biofilm and latest clinical methods to remove it.