China ; Humans ; Infant, Newborn ; Metabolism, Inborn Errors ; diagnosis ; therapy ; Neonatal Screening ; Phenylketonurias ; diagnosis ; Tandem Mass Spectrometry

Humans ; Lysosomal Storage Diseases ; prevention & control ; therapy ; Lysosomes ; metabolism

Cervical carcinoma is a serious threat to the health of women around the world ,and its inci-dence ranks at the second position after breast cancer in female reproductive system .In addition to oncogene acti-vation and inactivation of tumor suppressor gene ,endogenous and exogenous factors also affect the development of cervical cancer .

Helicobacter pylori is able to colonize the human stomach and dwell in the human stomach for decades or for whole lifetime. A number of potential virulence factors contribute to Helicobacter pylori to colonize this unusual niche. Motility is an essential colonization factor based on the fact that nonmotile variants of Helicobacter pylori can't infect gnotobiotic piglets. Motility is not a colonization factor based on rapid loss of motility of Helicobacter pylori in the gastric lumen in vivo. The exact roles of Helicobacter pylori motility are not yet known. The aim of this article is to discuss correlation between colonization and motility of Helicobacter pylori.

Some studies corresponding modernization of Chinese materia m edica(CMM)were performed in the institute for the new technologies,materials,and facilities based on the achievements and experiences gotten in the past two dec ades.The technologies of large-scale propagation of plant cell,tissue,and organ were applied to the products of cell metabolism,biotransformation,and artificial breeding and rooting.Many new types of large-scale bioreactors were designed an d used not only for laboratory but also for manufactory.Reaction/separation inte gration technology,microwave-assisted extraction,and the other new extraction te chnologies were studied to realize high efficiency and low energy.Besides tradit ional separation and purification methods,new technologies,including extraction in reversed micelles,one step three-phase extraction on Penicillin,foam fraction ation,membrane separation,and high-speed counter-current chromatography(HSCC),we re developed.HSCCC is a kind of liquid-liquid partition chromatography without a ny solid matrix,which eliminates irreversible adsorption of samples on solid sup port in the conventional chromatographic column.It has been successfully applied to the analysis and separation of various natural products.At the same time,man y types of microsphere and microencapsules for controlled release and separation media were prepared and lots of attention was paid on drug modifying and embedd ing techniques.HSCCC was also applied as a new method to the study of CMM finger print and showed good precision and repeatability.High performance liquid chroma tography(HPLC),gas chromatography(GC),high performance capillary electrophoresis (HPCE),and mass spectrometer(MS)played a very important role in fingerprinting.C ontinuous propagation-continuous collection technological process was set for la rge-scale propagation of micro-algae in the study of marine drugs.Transgenic alg ae were cultured to prduce genetically engineered products.

Education, Nursing, Continuing ; General Surgery ; education ; Humans ; Physicians

Objective Explore the changes of serum interleukin(IL) levels and its clinic significance in patients with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP).Methods The levels of IL-2, IL-4 and IL-6 in serum of 34 patients with DEACMP were measured by ELESA before and after treatment. As control, the levels of IL-2, IL-4 and IL-6 in 30 healthy persons and 30 patients with acute carbon monoxide poisoning(ACMP) were measured at same time.Results ⑴The levels of IL-2,IL-4 and IL-6 in serum of DEACMP patients before treatment were significantly lower than those after treatment(allP

Objective To study the influence of tumor necrosis factor-alpha (TNF-?) gene polymorphism on infections in old kidney allograft recipients. Methods The TNF-? genotype in -308 promoter position was determined in 87 old kidney transplant recipients from January 1998 to January 2001 by PCR using sequence -specific primer(PCR-SSP), and then the effects of genotypes on the frequency of rejection and infection, and the survival rate of the patient and graft were observed. Results 58 recipients were low TNF-? production genotype and 29 were high TNF-? production genotype. There were no significant differences in the frequency of rejection, median day of first infection and kinds of infections between the two genotypes. The frequency and times of infections were higher in low TNF-? genotype recipients than those in high TNF-? genotype recipients(67 2% vs 41 4%, P=0 021; 1 68 vs 0 86, P=0 017). The survival rates of the patients and grafts were equal in both the two groups. Conclusion The old recipients with low TNF-? production genotype may be susceptible to infections under routine immunosuppression. It is helpful for old recipients to perform more individual immunosuppression regimens according to TNF-? genotype.

By combining clinical observation and animal experiments and using FASCO - 3010 hemorheological devices to examine the relevant indices, it was found that no obvious changes can be found in the group treated with hormone before and after the treatment, whereas in the group with traditional drugs added, the RBC deformation, and specific viscosity of plasma were all markedly lowered. Comparison of rheological changes in SD rat showed that traditional Chinese drugs can markedly improve the hemorheology in such patients, indicating that these TCM treating principles can improve the concentrated, viscous, coagulated, agglutinated pathological blood status in SLE patients.

Root canal biofilm is frequently detected in the canal wall of infected root canal and the root canal with failed root canal therapy. Due to its special structure and diverse composition, root canal biofilm has the ability of the drug tolerance and antiimmunity, which lead to apical periodontitis. This review summarizes the features of the root canal biofilm and latest clinical methods to remove it.