Cloning, Molecular ; methods ; Dental Caries ; immunology ; prevention & control ; Forecasting ; Humans ; Research ; trends ; Research Design ; Vaccination ; Vaccines ; genetics ; immunology

OBJECTIVE:To put forward some suggestions for the development of e-commerce in China's pharmaceutical retail chains.METHODS:To drawn on experiences from the western developed countries in developing B to C pharmaceutical e-commerce and analyzed the problems existing in China's pharmaceutical industry.RESULTS & CONCLUSIONS:To develop e-commerce in China's pharmaceutical retail chains,the cooperated supervision of the concerned governmental departments is indispensable,in addition,the level of informatization should be improved step by step and the network of retail chain should be relied on to offer customer-centered services.

OBJECTIVE: To propose some suggestions towards the operation and development of online pharmacies in China.METHODS: From the following three aspects: website design,information provision,and customer service,the paper introduced and compared the websites of three approved online pharmacies,which include Jingwei Pharmacy,Shanghai Pharmacy and Jinxiang Pharmacy.RESULTS & CONCLUSIONS: To develop online pharmacies,the information display should be customer-centered,new marketing plans based on the characteristics of the internet should be developed;meanwhile,the operators of online pharmacies should help consumers developing an awareness of drug safety and offer them corresponding services.

Humans ; Periapical Periodontitis ; diagnosis ; etiology ; therapy ; Root Canal Therapy ; adverse effects

Humans ; Root Canal Obturation

Humans ; Root Canal Therapy ; instrumentation ; methods

Dental Pulp Cavity ; anatomy & histology ; Humans ; Root Canal Preparation ; methods

Dental Pulp Cavity ; abnormalities ; pathology ; Humans ; Root Canal Therapy ; methods

Objective To explore the effects of oxidative stress in porcine iliac endothelial cells(PIECs) induced by high glucose. Methods After being intervened by high glucose for some time, dihydroethidium (DHE) or dihydrorhodamine 123 (DHR123) was used as a reactive oxygen species(ROS) capture. The mean fluorescent intensity(MFI) of above probes which were the products of intracellular oxidation was detected by fluorescent microscopy and flow cytometry, and the level of ROS was thus measured. With lucigenin as chemiluminescence agent, luminescence changes were detected by chemiluminescence analyzer after addition of NADPH to observe the effect of high glucose on activity of NADPH oxidase(NOX). Results Intracellular MFI was markedly elevated with the concentration of high glucose and time of exposure to high glucose. It was revealed by flow cytometry that the NOX activity was significantly activated compared with normal medium treated PIECs(P

Objective To investigate the effect and underlying mechanism of Caudatin combined with Gefitinib on Gefitinib resistance induced by HGF in PC-9.Methods Model of EGFR-TKIs resistance in PC-9 cells was induced by exogenous HGF and co-cultured with MRC-5.Caudatin was tested as a drug resistant modulator to reverse the resistance of Gefitinib in PC-9 cells induced by HGF by MTT assay.Western blot was performed to observe the mechanism of Caudatin combined with Gefitinib reversing the resistance of PC-9 induced by HGF.Results The resistance of gefitinib to PC-9 was induced by exogenous HGF and co-cultured with MRC-5 which could reduce relative inhibitory rate ( P<0.05 ) .Neither caudatin ( 0-32 μM ) or Gefitinib (1μM) alone could significantly inhibit proliferation of PC-9 in the presence of HGF, which could be inhibited in a dose-dependent manner by Caudatin combined with Gefitinib ( P<0.05 ); Caudatin combined with Gefitinib down-regulated the phosphorylation levels of Met and PI3K/Akt simultaneously (P<0.05).Conclusion Caudatin could reverse the drug resistance of Gefitinib in PC-9 induced by HGF, the mechanism of which may be related to the inhibition of Met/PI3K/AKT pathway.