Cardiovascular Diseases ; etiology ; Chronic Disease ; Humans ; Hypertrophy, Left Ventricular ; etiology ; Kidney Diseases ; complications ; Risk Factors

ObjectiveTo study the optimized security strategy for informing cancer patients with the disease,and let patients be fully informed and even more actively cooperate with doctors while they are under treatment.MethodsGrouped with adversity quotient (AQ) score,these cancer patients were in comparison with those who had been informed by other ways without support strategy,and different groups of patients were estimated in different strategies in accordance with self-rating anxiety scale (SAS) and self-rating depression scale (SDS).Results With certain strategies,high and low AQ score groups' SAS and SDS scores stably restored in 1 day to 1 week,and the differences had statistical significance(P ＜ 0.01 ).The patients were well-informed without any difficulty and actively cooperated with treatment.No related factors led to self-mutilation,suicide and other serious incidents.While the SAS and SDS scores of the control group who were not informed by the strategy were not satisfactorily recovered,and the patients could not actively cooperate with the doctors.Especially being notified after 1 d to 1 week,the SAS and SDS scores had no significant improvement,and the differences had no statistical significance compared with themselves ( P ＞ 0.05 ).It is difficult to avoid the occurrence of serious incidents.Conclusion Based on AQ score,it is safe and effective to inform cancer patients of their disease condition by individual strategies.It not only respects patients rights,but also gains the active cooperation of the patients,and is helpful for treatment with minimizing adverse effects.

BACKGROUND:Occurrence and development of diabetic complications is closely related to the severity of oxidative stress imbalance in the body. OBJECTIVE:To investigate the effect of embryonic stem cel s on oxidative stress response of rats with diabetic nephropathy. METHODS:Primarily cultured rat embryonic stem cel s were observed for cel morphology and surface antigen detection. Sprague-Dawley rats were divided into experimental group (two injections of embryonic stem cel s via the tail vein), model group (injection of the same volume of PBS), and normal control group (with no modeling, intraperitoneal injection of sodium citrate-citrate buffer). In the former two groups, the rats were intraperitoneal y injected sodium citrate-citrate buffer diluted streptozotocin to establish diabetic nephropathy models before treatment. At 5 weeks after the last injection, blood glucose level, renal function indicators (urine protein/urine creatinine, blood urea nitrogen and serum creatinine) were tested in each group;contents of malondialdehyde and protein carbonyl were detected in the kidney;the expression level of superoxide dismutase was detected by western blot assay. RESULTS AND CONCLUSION:The embryonic stem cel s were oval or round, with clear boundary and good refraction, and highly expressed Oct-4 and SSEA-1. Compared with the control group, renal biochemical indicators, malondialdehyde and protein carbonyl contents were significantly increased, while the expression level of superoxide dismutase was decreased dramatical y in the model group and experimental group (P<0.05);compared with the model group, the renal biochemical indicators, malondialdehyde and protein carbonyl contents were dropped significantly in the experimental group, but the expression of superoxide dismutase was significantly rebounded (P<0.05). Taken together, embryonic stem cel s can reverse the occurrence and development of diabetic nephropathy by inhibiting oxidative stress in progress.

Objective To study the significance of serum human epididymis protein 4 ( HE4 ) and human cartilage glycoprotein-39(YKL-40) in the diagnosis of ovarian cancer .Methods 100 patients with ovarian cancer , 90 patients with benign tumor of ovary and 50 healthy people were enrolled .Serum HE4 and YKL-40 were detected . Results The positive rates of serum HE 4 in ovarian cancer group , ovarian benign tumor group and healthy group were 86.0%,5.5%,2.0%.The positive rate of serum HE4 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group (χ2 =31.06,30.82,all P<0.01).The positive rates of serum YKL-40 in ovarian cancer group ,ovarian benign tumor group and healthy group were 82.0%,3.3%,2.0%.The positive rate of serum YKL-40 in ovarian carcinoma was significantly higher than that in benign ovarian tumor group and the healthy group(χ2 =30.92,32.06,all P<0.01).Serum HE4 in the diagnosis of ovarian cancer ,the sensitivity was 86.0%,specificity of 95.7%,accuracy of 91.7%.Serum YKL-40 in the diagnosis of ovarian cancer ,the sensitivity was 82.0%,specificity of 97.1%,accuracy of 90.8%.For the joint detection,the sensitivity for diagnosis of ovarian cancer was 92.0%,and the specificity was 93.6%,accuracy was 95.0%,higher than those of the single detection . Conclusion Combined with serum HE 4 and YKL-40 detection can significantly improve the diagnostic accuracy of ovarian cancer patients .

Objective To study the relation between diabetic rat encephalopathy and hippocampal apoptosis, and the therapeutic effect and mechanism of Ginkgo biloba extract. Methods Sixty rats were assigned into control group(NC), diabetic encephalopathy group (DM+B) and EGb-treatment group (EGb) randomly, and every group was further divided into two time points of 1 month (NC_1 ,DM+B_1, EGb_1 ) and 3 month(NC_3 ,DM+B_3, EGb_3 ). The behavioral abnormalities were investigated by water maze test and the apoptosis levels of brain tissue by ELISA. Immunohistochemistry and RT-PCR were performed to determine the protein and mRNA expressions of Bcl-2 and Bax. Results (1) Mild dysfunction of learning and memory was detected in DM+B_1 group, and the dysfunction became worse in DM+B_3 group. The matters were greatly improved in EGb-treatment groups. (2) The expressions of Bcl-2 protein and mRNA were increased in DM+B_1 and DM+B_3 groups, and were stronger in EGb_1 and EGb_3 groups. (3) The expressions of Bax protein and mRNA were obviously increased in DM+B_1 and DM+B_3 groups, and were dramatically reduced in EGb_1 and EGb_3 groups. (4) Compared with control group, the apoptosis level of brain tissue was increased in DM+B_1 group, and obviously greater in DM+B_3 group. In the EGb-treatment groups the levels were dramatically reduced. Conclusions Diabetic encephalopathy may be closely related to hippocampal apoptosis. EGb has an protective effect and the mechanism may be attributed to its effects inhibiting apoptosis.

Establishing the assessment methods of childhood asthma is highlighted in asthmatic prevention and treatment.Due to the factors such as age,genotypic environment,pathogenesis and treatment,the assessment methods existed have some defects and cant objectively reflect the situation of asthma disease and airway inflammation.The assessment methods including symptom scores,pulmonary function test,airway responsiveness detection and fractional concentration of exhaled nitric oxide are described in this review.The principles,advantages,disadvantages,application ways and so on are discussed in detail,to improve the rationality of the application in these methods in order to make them play a greater role in asthmatic diagnosis,disease detection,medication guild and prognosis estimation,and explore a more effective and simple assessment way in children asthma.

Leukemia inhibitory factor(LIF) is a multifunctional cytokine.In recent years,investigations have made some advancement in the effect of LIF on central nervous system.LIF shows a high expression in various central nervous system diseases and related animal models,which shows a neuroprotective effect most time in central nervous system injuries.LIF is defined as a neurotrophic factor.It might provide a new strategy for the therapy of central nervous system diseases to undertake further studies on leukemia inhibitory factor and central nervous system diseases.

Objective To evaluate the results of palliative procedures combined with intervention techniques for tetralogy of Fallot with severe hypoplastic pulmonary artery.Methods From December 2002 to Decembre 2009,complete repair of tetralogy of Fallot were performed in 1586 patients including 18 cases ( 12 male and 6 female) with severe hypoplastic pulmonary artery after 8 - 40 (20 + 11 ) month of palliative operations.The patients'age and body weight were 12 - 72 months and 8.0 - 16.5 kg at the time of complete repair.Associated cardiac anomalies include 3 cases of atrial septal defect,2 cases of absence of left pulmonary artery,1 case of the left pulmonary artery arising from the aorta,1 case of left superior vena cava.Major aortopulmonary collateral arteries were confirmed by angiography in 7 patients.Palliative operation was performed once in 13cases,twice in 4 cases and thrird in 1 patient before complete repair.Palliative operations included 14 procedures of Blalock-Taussig shunt,6 of Waterston shunt,3 of right ventricle outflow tract reconstruction and 3 of balloon pulmonary valvuloplasty.Major aortopulmonary collateral arteries were ligated in 4 cases,unifocalized in 1 and interventional embolized in 2 before complete repair.Results There was no early death.One reoperation was performed because of the obstruction of vascular prosthesis in the next day of shunt operation.Nakata index [ (84 ±40) versus (200 ±81 )] and McGoon ratio were increased after complete repair as compared with before operation [ ( 1.14 + 0.33 ) versus ( 1.77 + 0.51 ),P ＜ 0.001 ].Hemoglobin decreased from ( 175 ±46) g/L to ( 149 ± 15 ) g/L (P ＜0.05) and peripheral oxygen saturation increased from (71 ±9)％ to ( 86 ± 5 ) ％ ( P ＜ 0.05 ).Complete repair were performed in all 18 patients eventually.Conclusion Palliative procedures combined with intervention techniques may ameliorate the growth of pulmonary artery effectively and improve the results of complete repair of tetralogy of Fallot with severe hypoplastic pulmonarv arterv.

Objective To explore the application value of evidence-based nursing in prevention of lower limb deep vein thrombosis after hip arthroplasty. Methods 41 cases of patients undergoing hip arthroplasty were selected in our hospital from January 2008 to June 2011.28 cases of patients who took evidence-based nursing were set as the observation group,and the 13 cases of remainders who received routine nursing were named as the control group.The treatment results were compared between the two groups. Results The incidence of deep venous thrombosis was 23.1％ in the control group,significantly higher than the observation group.In the observation group,FIB value,D-D values,CD62p value,DQ values,ZQ value and GQ value were lower,and the PC value was higher than the control group. Conclusions Application of evidence-based nursing in hip arthreplasty can significantly reduce the incidence of lower limb deep vein thrombosis,which is worthy of clinical application.