Objective:To explore the role of hypoxia in C_2C_(12) cells proliferation.Methods:We chose 10% oxygen concentration.Cell numbers were counted with a hemacytometer.The proliferous indexes were observed with a flow cytometer.HIF-1? protein was determined by Western blot,mRNA expression was determined with reverse transcriptase-polymerase chain reaction(RT-PCR).Results:The cell numbers and proliferous indexes were higher(P

Objective To investigate the expression of Human leucocyte antigen(HLA)-DP,-DQ,-DR and CD40 in human retinal pigment epithelial(RPE)cells,to determine their molecule expression in immune response process,and their abilities to stimulate T lymphocyte activation. Methods Human RPE cells were cultured with or without(-IFN respectively.Expression of HLA-DP,-DQ,-DR and CD40 was measured by immunohistochemical staining.Meanwhile,peripheral blood mononuelear cells(PBMC)were co-cultured with RPE cells in vitro,and then the expression of activated lymphocytes CD69 was measured by fluorescence activated eell sorter(FACS). Results Expression of HLA-DP,-DQ,-DR and CD40 antigen were enhanced by γ-interferon inducement.Increasing amount of CD69 positive Iymphocytes were found in the co-culture system of RPE cells and PBMC. Conclusion T-lymphocytes in the peripheral blood were activated by human RPE cells which is antigen presenting cells with immunological characteristics potential.

Objective:To investigate the capability of corneal cells to stimulate lymphocyte proliferation and the expression of costimulatory molecules on primary human corneal epithelial cells.Methods:Human corneal epithelial cells were cocultivated with peripheral blood mononuclear cells(PBMC). Expression of HLA class Ⅱ antigens, CD40, CD154, CD80 and CD86 was measured by immunohistochemical staining,and the expression of such molecules above on rejected corneal grafts was contrastingly assessed. Activation of lymphocytes was determined by measuring upregulation of CD69 by FACS analysis.Results:HLAⅡ expression was detectable on human primary corneal epithelial cells,and furthermore, upregualtion of costimulatory molecules CD40 and CD80 were also found after induction with ?-interferon. Both of HLA and CD40 were expressed on rejected corneal grafts. T lymphocytes were activated by epithelial cells in the coculture system.Conclusion:Human corneal epithelial cells are involved in rejection process of the corneal transplantation. The immunological reaction is triggered by epithelial cells,and endothelial cells suffer as the targets.

Objective To construct the clone vector of S gene in occult hepatitis B virus infection.To analyse its mutations and to construct its prokaryotic expression vector.Methods To amplify the HBV S gene with polymerase chain reaction(PCR),recombinate the S gene and vector PUC18,sub-clone the recombination and construct its prokaryotic expression vector with pThiohis B. Results There were two samples negative with positive HBV DNA from 64 samples with negative hepatitis B surface antigen.The clone vector and prokaryotic fusion protein expression vector of an occult HBV infection′s S gene were successfully constructed.The S gene was not changed by sequencing analysis.Conclusion The fluorescence quantitive polymerase chain reaction (FQ-PCR) can examine low-copies in occult hepatitis B virus infection.Determining quantitivly HBV DNA of recombination can identify the recombination and display multiplication state intuitirely.It is very successful in constructing the clone vector and prokaryotic fusion protein expression vector.

Objective To discuss the clinical application of preoperative transcatheter arterial chemoembolization (TACE) in treating squamous cell carcinoma of tongue. Methods A total of 26 patients with pathologically proved squamous cell carcinoma of tongue were enrolled in this study. Using Seldinger technique, lingual artery angiography and TACE were carried out in all patients. Radical resection of tongue cancer was performed in 2-3 weeks after TACE. Results Super-selective catheterization and TACE was successfully accomplished in all patients. After TACE the tumor size was significantly decreased in all patients. During the operation, clear lesion's border was observed, and the amount of blood loss was less than usual. Conclusion For the treatment of squamous cell carcinoma of tongue, preoperative superselective TACE is safe and effective;it can increase the opportunity of surgical resection, reduce the surgical damage, and improve the quality of life as well as the survival rate.

The authors give an account of the basic concept and classification of cytotoxic drugs. A center for compounding cytotoxic drugs has been set up in their hospital by using for reference the management experience of the US in this aspect. The center stresses centralized management in compounding cytotoxic drugs, strict operating rules and protective actions, and reduction of possible toxic damages resulting from cytotoxic drugs and infection caused by them in other sectors, thus guaranteeing the physical and mental health of the medical staff. At the same time, the authors call on all medical institutions to pay attention to the disposition of cytotoxic drugs and exert efforts to help a relevant national standard to come out as soon as possible.

Objective To explore the structural foundation of transport pathway of pineal secretions from the pineal body to the cerebrospinal fluid of subarachnoid space. Methods The pineal capsule of the superficial pineal body of 1.5-month and 12-month old rats was observed under scanning electron microscope(SEM).Results Cribriform and trumpet-shaped epithelial stomata were scattered on the pineal capsule.Cribriform epithelial stomata were seen mostly in 1.5-month old rats.They were composed of many round pores which pierced through the full-thickness of the periphery of the capsule endothelium.The pores were dense and ranged from 200-500 nm in diameter;trumpet-shaped epithelial stomata were seen in both 1.5-month and 12-month old rats.Trumpet-shaped epithelial stomata were located among epithelial cells of the pineal capsule.They were round or elliptic in shape and ranged from 1-4 ?m in diameter.On the surface of the pineal capsule,many secretory granules were observed.They were spherule and 8001 000 nm in diameter.Conclusion The releasing pattern of secretory granules of the pineal body could include a releasing of the whole membrane;the pineal secretions may be transported directly from the pineal body to the cerebrospinal fluid of subarachnoid space through the epithelial stomata of the pineal capsule.

Objective: To study the relationship between explicit/implicit self-esteem and mental health. Methods: 49 college students (including 26 male and 23 female, mean age 22) completed Rosenberg Self-esteem Scale and SCL-90. Their implicit self-esteem was measured by Implicit Association Test (IAT). Results: The results indicated a moderate to strong correlation between explicit self-esteem and mental health level. Yet the implicit indicator show insignificant influence on mental health. Conclusion: The study tends to support the hypothesis that implicit self-esteem is a independent structure from explicit self-esteem

Complications of vascular access are main component of vascular complication in percutaneous cardiovascular intervention.The common complications of femoral access may include hemorrhage and hematoma at access site,vasovagal reflex,pseudoaneurysm,arteriovenous fistula,retroperitoneal hematoma and deep venous thrombosis.The common complications of radial access may include radial artery spasm,radial artery occlusion,vascular injuries or hematoma at forearm and other sites,and osteofascial compartment syndrome.The common complications of ulnar and brachial accesses may include hemorrhage and hematoma at access site,upper limb and hand ischemia,and nerve injuries.Effective prevention and treatment of vascular access complications are key steps to minimize the incidence and hazards of vascular complications in percutaneous cardiovascular intervention.

Objective: To improve the digital image system's retrieval speed and transmission efficiency, change the defect of traditional preservation and improve diagnostic quality of the image. Methods:Medical information transmission based on the socket can make the multi-threading parallel work to improve the running speed of the program, so images and files can be transmitted quickly. Results:Socket-based multi-threading technology is applied to the hospital departments and various remote medical system, that will improve the transmission speed and save time and money. Conclusion: Medical information transmission based on the socket can manage and application the medical imaging effectively.