[Objective]To observe the effect of vacuum sealing drainage(VSD) on bacteria and microcirculation of acute explosion wounds in pigs.[Method]Twelve blast wounds,created by explosion of a specific type of electric detonations which were fixed at 1 cm over the skin of the hips of six Lanzhou healthy pigs(30-40 kg),were divided into two matched groups.Debridement was performed at 6 hours after injury.Group A was treated with conventional dressing change,and group B was treated with VSD under the pressure of-20kPa.Tissue specimens collected from different sites of the wound bed before and at 12,24,48,72 hours after treatment,and experienced bacteria counting.The microcirculation of the blast wounds was examined by color B-mode ultrasonic diagnostic equipment before and 72hours after treatment.[Result]The difference of wound bacteria loads between group A and group B was statistically signficant at different time points.Bacteria loads in group B were increased to 105cfu/g at 48 h after treatment,compared with group A at 24 h.No signal of blood flow in blast wound was found before treatment.In contract,the blast wound had plenty of signals of blood flow in group B.[Conclusion]VSD could reduce the bacteria loads,and delay the time of infection.Meanwhile it improved the microcirculation of the acute blast wound.

Objective To analyze the clinical effects of bronchoscope combined with hot and cold ablation in treatment of benign and malignant tumors in primary tracheal and bronchial. Methods From January 2013 to November 2015, 30 cases of pathological types of primary tracheal / bronchial benign and malignant tumors patients include: 8 cases of hamartoma, 1 case of lipoma, 4 cases of granuloma, 1case of clear cell carcinoma, 7 cases of small cell lung cancer, 9 cases of squamous cell carcinoma, were treated by using carbon dioxide cryotherapy combined hot ablation under bronchoscope. Then investigated the outcomes. Results 30 patients, 22 males, 8 females, suffered from 1 month to 2 years, underwent surgery bronchoscope interventional treatment under local anesthesia combined with intravenous anesthesia, are taking joint cold ablation technique. All patients airway is completely opened, the lifting of the airway obstruction. Including 19 cases of disposable surgical removal of the lesion; 8 cases of lesions due to wide distribution, 2 times surgery; three cases three times surgery, no serious complications, follow-up without recurrence, blood gas analysis and dyspnea index were significantly improved. Conclusion Bronchoscope cold joint ablation techniques in the treatment of primary tracheal, bronchial benign and malignant tumors is effective, simple, minimally invasive and safe.