Objective: To design and synthesize a novel vector for rhBMP2 delivery system in tissue engineering. Methods:Dextran glycidol methacrylate(dex-GMA) was synthesized with dextran(dex) and glycidol methacrylate(GMA).Dex-GMA microspheres were prepared by suspension polymerization. The swelling behavior of the microspheres was evaluated by the swelling equilibrium parameter Q. The biodegradation properties of the dextran-based hydrogel microspheres were assessed by the surface morphology before and after biodegradation. Results:Microspheres of dex-GMA in the size(diameter) of 20 to 80 ?m with good configuration were produced. Q value of the microspheres with the diameter of 20-30 ?m was 10.9?3.3,that of those with 70-80 ?m 8.9?6.4.Stirring speed, span-80(emulsifer) quantity and the proportion of dex-GMA affected the size of the microspheres. rhBMP2 was enveloped into the microspheres. Complete degradation of the microspheres was observed during 20 to 40 days at 37 ℃ in normal saline. Conclusion: Dex-GMA hydrogel microspheres may be a release controlling system for rhBMP2 delivery.

Objective To investigate the clinical and pathological features of Wegener granulomatosis to improve the management of it. Methods Twenty-three patients with Wegener granulomatosis were analyzed retrospectively. Results The average age at onset was 37.2 years (range from 20~57 years). The average interval from clinical symptoms presentation to diagnosis was 5.8 months. The initial symptom of 23 cases was the upper respiratory tract involvement (48%). The patients had multiple system and organ involvement. 87% patients with WG had lung involvement and 78% patients had glucocorticoids. Seventeen out of 23 were cANCA (74%) positive. The pathological manifestations included necrotizing granulomatosis and vasculitis. Conclusion Clinical manifestation of WG is complicate, including multiple system and organ involvement. The upper and lower respiratory tract and kidney are the most commonly involved organs. Necrotizing granulomatosis and vasculitis are the pathological features of WG.

Objective To detect the level of cytokines and their receptors secreted by peripheral blood CD8 + T lymphocytes from vitiligo patients,and to evaluate the influence of tea polyphenols on their secretion.Methods CD8+ T lymphocytes were isolated from the peripheral blood of 12 patients with progressive vitiligo,12 patients with stable vitiligo and 10 healthy controls,and cultured in vitro for 20 days.Then,some CD8+ T lymphocytes were treated with tea polyphenols of 100 mg/L for two days.Enzyme-linked immunosorbent assay (ELISA) was carried out to determine the levels of tumor necrosis factor (TNF)-α,interferon (IFN)-γ and interleukin-2 receptor (IL-2R) in the culture supernatants of CD8+ T lymphocytes before and after the treatment with tea polyphenols.Analysis of variance and t test were conducted to assess differences in these parameters among these groups and changes between pretreatment and posttreatment,respectively.Results There was a sequential decrease in the level of TNF-α ((191.302 ± 6.077) vs.(175.966 ± 2.467) vs.(173.664 ± 3.600) ng/L,F =4.784,P ＜ 0.05),but a sequential increase in that of IFN-γ ((280.182 ± 36.070) vs.(371.670 ± 24.352) vs.(447.147 ± 8.432) ng/L,F=9.036,P＜ 0.01) and IL-2R ((8.375 ± 0.161) vs.(8.845 ± 0.161) vs.(9.345 ±0.125) ng/L,F =9.639,P ＜ 0.01) in the culture supernatant of CD8+ T lymphocytes from patients with progressive vitiligo to patients with stable vitiligo and healthy controls.After two days of tea polyphenol treatment,a statistical decrease was observed in the level of TNF-α in the culture supernatant of CD8 + T lymphocytes from patients with progressive vitiligo ((164.797 ± 1.784) ng/L,P ＜ 0.01),patients with stable vitiligo ((166.150 ± 3.576) ng/L,P ＜ 0.05) and healthy controls ((155.028 ± 5.759) ng/L,P ＜ 0.05),but no statistical changes were noted for IFN-γor IL-2R (all P ＞ 0.05) despite a slight elevation in the level of IFN-γin the culture supernatant of CD8+ T lymphocytes from patients with stable vitiligo and in that of IL-2R from all the three groups as well as a mild reduction in that of IFN-γ from patients with progressive vitiligo and healthy controls.Conclusions The changes of cytokines and their receptors secreted by CD8 + T lymphocytes may be associated with the induction and progression of vitiligo.Tea polyphenols may treat vitiligo via suppressing the secretion of TNF-α by CD8+ T lymphocytes.

Objective To evaluate the role of miR219‐2‐3p in gastric cancer and the underlying mechanisms .Methods Real time RT‐PCR was employed to quantify the expression level of miR219‐2‐3p in matched tissues of 82 patients .In vitro cell proliferation , the expression of tumorgenesis related protein ERK1/2 was performed by overexpressing miR‐219‐2‐3p in gastric cancer cell line MGC803 .Results The expression level of miR‐219‐2‐3p significantly decreased in late stage gastric cancer (P< 0 .05) .The cell proliferation rate was significantly reduced in gastric cancer cell lines overexpressing miR‐219‐2‐3p .Furthermore ,the expression level of p‐ERK that activated form of ERK was significantly decreased whereas the total level of ERK was unchanged after overex‐pressing of miR‐219‐2‐3p in MGC803 cell lines .The expression level of p‐ERK was also increased in gastric cancer tissues .Conclu‐sion miR‐219‐2‐3p may acts as a tumor suppressor in gastric cancer by decreasing the activity of ERK 1/2 signaling pathway .

OBJRCTIVE:To study the feasibility and the preparation of bone morphogenetic protein-2(BMP 2 )carrying gel microspheres by dextran-glycidyl methacrylate(DEX-GMA)and to make an initial study on the drug-loading and drug releasing function of gel.METHODS:The orthogonal test was conducted with the reaction temperature,the addition of DEX-GMA and Span-80,the stirring speed and so on as investigation factors,the best preparation technics of BMP 2 -DEX-GMA gel microspheres was optimized and the finished products of which were given an initial determination.RE-SULTS:The BMP 2 -DEX-GMA gel microspheres preparation could be made by suspension polymerization,the optimized preparation technics including the reaction temperature was30℃,the addition of DEX-GMA and Span-80were0.6%and0.06%respectively,the stirring speed was300r/min,the BMP 2 -DEX-GMA gel microspheres from the above formulation take good shapes with the particle diameter at20?m～50?m,the envelopment ratio was(78.52?4.34)%,the quantity of drug-loading was(10.68?1.34)%,and the swelling ratio was85.9%,which has a good stability and redispersibility.CON-CLUSION:The preparation technics of gel microspheres drug-loading system is simple and the loading dosage is high,which can be used as a bioactive drugs carrier.

Objective To investigate the arterial injury in vitro porcine kidney to different size of nephrostomy tracts. Methods The technique of percutaneous nephrostomy was applied to establish 11 groups of different size of nephrostomy tracts from 12 F to 32 F,with 40 tracts in every group.The technique of digital subtraction angiography (DSA) was used to inspect and analyze arterial injury. Results In the range from 12 F to 32 F,the damage of arterial injury increased with the size of the tract diameter.In groups of 20 F and 32 F,the number of nephrostomy tracts with serious arterial injury was 18 (18/40) and 30 (30/40) respectively,and the difference was statistically significant (P＜0.05).There was no statistical difference between groups 18 F、20 F、and 22 F. Conclusions The damage of renal arterial injury increased with the size of the nephrostomy tract.Atract of 20 F reduees arterial injury compared with a tract of 32 F.

From September 2002 to May 2006, five patients ( age range, 24 to 46; mean, 32) with tibial defect underwent transplantation of free latissmus dorsi osteocutaneous flap using bridge-like vascular anastomosis. All the osteocutaneous flaps survived without any serious complications, and tibial defects were improved completely. After 1.5 to 4.5 years' follow-up ( mean, 2.6), no remarkable dysfunction was found at donor sites, and local injury was reduced. This study indicates that transplantation of free latissmus dorsi osteocutaneous flap using bridge-like vascular anastomosis might be useful in leg reconstructive surgery if only one vessel is remained.

0.05)between DA and DE when the diameter of gel microspheres was20?m～40?m.CONCLUSI_ ON:The drug-loading pattern of BMP 2 -DEX-GMA gel microspheres can be influenced by its particle size,but its drug release character can not be changed,the controlled release of drugs can be achieved through changing the nature of its drug-loading material.

Objective : To investigate the prevalence of hepatitis B virus ( HBV ) infection among volunteer blood donors in Hangzhou City, and to evaluate the residual risk of transfusion-transmitted HBV infections. Methods : Data pertaining to volunteer blood donors in Hangzhou City from 2016 to 2019 were retrieved from the blood donor management system. Hepatitis B surface antigen ( HBsAg ) was detected by enzyme-linked immunosorbent assay ( ELISA ) and HBV DNA was detected using nucleic acid testing. The incidence/window period model was employed to assess the residual risk of HBV transmitted through transfusion from donors. Results : The prevalence of HBV infections was 0.56% among the 320 755 first-time donors and 0.13% among the 279 816 repeat donors in Hangzhou City from 2016 to 2019, and a higher prevalence of HBV infection was detected among first-time donors than among repeat donors ( P<0.05 ). The residual risks of transfusion-transmitted HBV infection were 296.38 per million person-times ( 95%CI: 277.57 to 315.19 per million person-times ) and 98.79 per million person-times ( 95%CI: 87.15 to 110.43 per million person-times ) among first-time and repeat donors with positive HBsAg, and were 86.79 per million person-times ( 95%CI: 76.60 to 96.98 per million person-times ) and 28.93 per million person-times ( 95%CI: 22.63 to 35.23 per million person-times ) among first-time and repeat donors tested positive for HBV DNA, respectively. Conclusions There is still a residual risk of HBV infection transmitted through transfusion from blood donors in Hangzhou City. Nucleic acid testing may remarkably reduce the residual risk of transfusion-transmitted HBV infection in blood donors.

OBJECTIVETo analyze the molecular genetic basis for a case of ABw07 phenotype of ABO subtype.

METHODSThe ABO group antigens on red blood cells of the proband were identified by monoclonal antibodies and the ABO antibody in serum was detected by the standard A, B and O cells. The exons 6 and 7 of the ABO gene was amplified by PCR and the PCR product was sequenced directly after enzyme digestion. The amplified product was also cloned by TOPO TA cloning sequencing kit to split the two alleles apart, chosen colonies were sequenced bidirectionally. The samples of the parents and one sister of the proband were collected, then the blood group serological test and sequence analysis for exon 6 to 7 of ABO gene were performed.

RESULTSBoth A and B antigen were detected on red blood cells of the proband and there was anti-B antibody in the serum. There was no 261G deletion. And the 297A/G, 467C/T, 526C/G, 657C/T, 703G/A, 796C/A, 803G/C, 930G/A, 1055G/A and 1096A/G loci were heterozygotes by direct DNA sequencing, which can be assigned for A102Bw07 genotype. After cloning and sequencing, two alleles of A102 and Bw07 were obtained. The Bw07 has one nucleotide change of G to A at nucleotide 1055 compared with B101, which results in an amino acid change from Arg to Gln at nucleotide 352. The Bw07 in the proband was inherited from his mother, and the serological characteristic of the ABO blood group and the sequence of exons 6 and 7 of the mother were the same as that of the proband.

CONCLUSIONThe G to A at nucleotide 1055 of alpha -1, 3 galactosyltransferase gene (B gene) can result in BW7 phenotype, with anti-B antibody in serum.

ABO Blood-Group System ; genetics ; Alleles ; Base Sequence ; Blood Grouping and Crossmatching ; Cloning, Molecular ; Female ; Heterozygote ; Humans ; Male ; Molecular Biology ; Phenotype ; Sequence Analysis, DNA ; Young Adult