1.Sequence Analysis of Hypothetical Proteins from Helicobacter pylori 26695 to Identify Potential Virulence Factors.
Ahmad Abu Turab NAQVI ; Farah ANJUM ; Faez Iqbal KHAN ; Asimul ISLAM ; Faizan AHMAD ; Md Imtaiyaz HASSAN
Genomics & Informatics 2016;14(3):125-135
Helicobacter pylori is a Gram-negative bacteria that is responsible for gastritis in human. Its spiral flagellated body helps in locomotion and colonization in the host environment. It is capable of living in the highly acidic environment of the stomach with the help of acid adaptive genes. The genome of H. pylori 26695 strain contains 1,555 coding genes that encode 1,445 proteins. Out of these, 340 proteins are characterized as hypothetical proteins (HP). This study involves extensive analysis of the HPs using an established pipeline which comprises various bioinformatics tools and databases to find out probable functions of the HPs and identification of virulence factors. After extensive analysis of all the 340 HPs, we found that 104 HPs are showing characteristic similarities with the proteins with known functions. Thus, on the basis of such similarities, we assigned probable functions to 104 HPs with high confidence and precision. All the predicted HPs contain representative members of diverse functional classes of proteins such as enzymes, transporters, binding proteins, regulatory proteins, proteins involved in cellular processes and other proteins with miscellaneous functions. Therefore, we classified 104 HPs into aforementioned functional groups. During the virulence factors analysis of the HPs, we found 11 HPs are showing significant virulence. The identification of virulence proteins with the help their predicted functions may pave the way for drug target estimation and development of effective drug to counter the activity of that protein.
Carrier Proteins
;
Clinical Coding
;
Colon
;
Computational Biology
;
Drug Discovery
;
Gastritis
;
Genome
;
Gram-Negative Bacteria
;
Helicobacter pylori*
;
Helicobacter*
;
Humans
;
Locomotion
;
Sequence Analysis*
;
Stomach
;
Virulence Factors*
;
Virulence*
2.Obstacles and Facilitators in Daily Living Activities Among Persons with Spinal Cord Injury: A Systemic Review
Faizan Jameel Khanzada ; Mohammad Ghazali Masuri ; Erna Faryza Mohd Poot ; Mohd Zulkifli Abdul Rahim ; Ahmad Zamir Che Daud
Malaysian Journal of Medicine and Health Sciences 2024;20(No.1):271-280
Introduction: This systematic review aimed to investigate the level of participation, obstacles, and facilitator factors
that influence activities of daily living among persons with spinal cord injury (SCI). Methods: A comprehensive
search was conducted in four online databases, namely Google Scholar, PubMed, OT Seeker, and Cochrane Library
covering the ten-years period from January 2012 to December 2022. Inclusion criteria encompassed original published studies in English focusing on daily activities, work, participation, obstacles, and facilitators in persons with
SCI. Non-peer review sources (e.g., abstracts, grey literature, preprints), and studies unrelated to occupational therapy were excluded. The selected studies were assessed for quality using McMaster University Occupational Therapy
Evidence-Based Practice critical review form. Results: Out of the 678 articles identified, ten studies were included
after the screening, exploring participation in daily living activities, employment, return to work, leisure activities,
family tasks, and community mobility among persons with SCI. Obstacles and facilitators influencing participation
in activities of daily living were classified using the International Classification of Functioning, Disability, and Health
(ICF) framework. This review highlighted that long-term participation is challenging for persons with SCI, affected
by obstacles such as body functions, pain, low self-esteem, and environmental and social factors. Conclusion: The
findings underscore the importance of adopting a multidisciplinary rehabilitation approach to enhance participation
in daily activities for persons with SCI. Occupational therapy plays a significant role in improving participation levels
among persons with SCI.