1.Mutational Screening of Zuelacmycin-producing Strain RL-2
Li-Xia GOU ; De-Rong AN ; Shuang-Fa LIU ; Jing LI ;
Microbiology 2008;0(08):-
In this research, the zuelacmycin-producing strain Streptomyces venezuelaevar. qinlingensis RL-2 was used as original strain, the spore suspension of which was induced by UV, LiCl and the compound treatmeat (UV+LiCl) respectively. The zuelacmycin-high-yield strain UVL-108 was obtained by the treatment that the exposure time under UV irradition was 45 s and the concentration of LiCl was 0.4%. The heredity characters of mutant UVL-108 were stable in succesive six generations. The antibacterial activity and the fermentation titer of mutant UVL-108 were determinded by bidirectioned culture and mycelial linear growth respectively. The results demonstrated the antibaterial activity of mutant UVL-108 was improved by 77%, and the relative toxicity of fermentation to Phyricularia grisea was improved by two times compared with the original strain.
2.Screening and Identification of a New Elastase-producing Strain
Shuang-Fa LIU ; De-Rong AN ; Li-Xia GOU ; Na LI ;
Microbiology 2008;0(09):-
The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw,and the selected strain was identified. The screening strategy included casein(skim milk) plate selecting and elastin(beef tendon) re-screening. And then,the morphological,physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally,the strain LSF-97 which had a excellent decomposing ability to beef tendon was obtained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under the phylogenetic tree,the morphological and physiological and biochemical characteristics are also consistent with pattern of bacteria. So it was identified as Bacillus pumilus.
3.Changs of B7 Family Costimulatory Molecules and Their Receptors Expression in Children with Asthma
ying, ZU ; cheng-rong, LI ; de-fa, LI ; yue-jie, ZHENG ; jing, LI
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To explore the possible role of B7 family cell surface costimulatory molecules and their receptors in immunopathogenesis in children with asthma.Methods Forty children with acute exacerbation of asthma and 36 cases of age-matched healthy children were enrolled in the study.Interferon(IFN-?),IFN-? and lipopolysaccharide(LPS),phorbol 12-myristate 13-acetate and ionomycin or zero were used to stimulate peripheral blood mononuclear cells,flow cytometry was used to detect B7-1 and B7-2,B7H1,B7DC and B7H expression on CD14+ cells;CD4+ cells were separated by immune beads and stimulated by phorbol-12-myristate-13-acetate and ionomycin and fluorescence quantitative polymerase chain reaction was used to detect CD28,cytotoxic lymphocyte associated antigen-4(CTLA-4),programmed death-1(PD-1)and inducible costimulator(ICOS) mRNA expression.Results 1.The levels of B7-1 and B7-2 expression in children with asthma were higher than those of healthy control group [B7-1:(6.68?3.97)% vs(1.74?0.69)%;B7-2:(10.87?5.99)% vs(1.58?0.75)% Pa
5.Relationship between Imbalance of Suppressors of Cytokine Signaling 1/Suppressors of Cytokine Signaling 3 and Abnormal Activation of Monocytes/Macrophages in Children with Asthma
ying, ZU ; cheng-rong, LI ; de-fa, LI ; yao-jie, ZHENG ; hong-ling, MA
Journal of Applied Clinical Pediatrics 2006;0(16):-
Objective To investigate the relationship between the imbalance of suppressors of cytokine signaling 1(SOCS1)/SOCS3 and abnormal activation of monocytes/ macrophages in children with acute-phase asthma, and explore the molecular mechanism of chronic inflammatory process on airway.Methods The present study enrolled 20 asthmatic children and 20 age-matched normal children. Dual-color flow cytometric analysis was performal to detect the percentage of CD_ 80 、CD_ 86 expressing on CD_ 14 ~+ cell. Reverse transcriptase-polymerase chain reaction and real-time PCR were used to analyze SOCS1,SOCS3 expression in monocytes/ macrophages.Results The proportions of CD_ 80 ,CD_ 86 expressed on CD_ 14 ~+ cell in children with asthma were significantly higher than those in control subjects(CD_ 80 :7.0% vs 1.70%),(CD_ 86 :11.37% vs 2.03%),all P
7.The morbidity of pneumoconiosis in Jiaozuo city.
Zhen-rong MIAO ; Xiao-fa YANG ; Lian-xia GUO ; Shu-xia SHI ; Fang-li DU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(4):298-299
Adult
;
China
;
epidemiology
;
Coal Mining
;
Humans
;
Middle Aged
;
Morbidity
;
Pneumoconiosis
;
epidemiology
8.mRNA expression and clinical significance of cancer-testis antigen GAGE gene in hepatocellular carcinoma.
Fei-lan ZHAO ; Shao-jian HE ; Peng LI ; Fa-rong MO ; Rong FAN ; Ling LAN ; Guo-rong LUO ; Xiao-xun XIE
Chinese Journal of Hepatology 2006;14(8):605-606
Adult
;
Antigens, Neoplasm
;
genetics
;
Carcinoma, Hepatocellular
;
genetics
;
pathology
;
Female
;
Humans
;
Liver
;
pathology
;
Liver Neoplasms
;
genetics
;
pathology
;
Male
;
Middle Aged
;
RNA, Messenger
;
genetics
9.Investigation report of the effect of control measures to iodine deficiency disorders in Xining in 2009
Li-lin, CHEN ; Duo-long, HE ; Shu-bang, LI ; Fa-rong, ZHANG ; Xun, CHEN ; Pei-chun, GAN ; Zhi-jun, ZHAO
Chinese Journal of Endemiology 2011;30(1):81-83
Objective To master iodine nutritional status of people after universal salt iodization in Xining that reached the stage goal of elimination iodine deficiency disorders. Methods In the 7 counties investigated of Xining in 2009, 5 towns were randomly selected in each county, and one school was randomly selected in each town, 80 children aged 8 to 10 were randomly selected in each school, and goiter were examined, urinary iodine and salt iodine were tested. Thyroid gland goiter of children was detected by thyroid palpation, children's urinary iodine was tested by As( Ⅲ )-Ce4+ catalytic spectrophotometry, and salt iodine was tested by direct titration. Results A total of 2919 children aged 8 to 10 were examined, 31 goiter was detected, goiter rate was 1.06%(31/2919).One thousand and seventy-eight urine samples were detected, urinary iodine median was 205.3 μg/L, that lower than 20 μg/L accounted for 1.9% (20/1078), lower than 50 μg/L accounted for 4.5%(48/1078). Two thousand and seventy-nine salt samples were detected, median of salt iodine was 32.80 mg/kg, the rate of non-iodized salt was 0.87%(18/2079), the coverage rate of iodized salt was 99.13%(2061/2079), the qualified rate of iodized salt was 98.64% (2033/2061), the consumption rate of qualified iodized salt was 97.79% (2033/2079). Conclusions Prevention and control of iodine deficiency disorders has achieved remarkable results in Xining city, all indicators have reached the national standard to eliminate iodine deficiency disorders.
10.Changes in cerebral blood flow during mastication in patients receiving prosthesis insertion for repairing maxillary defect.
Ya-juan GUO ; Hong-chen LIU ; Li SUN ; Rong-fa BU
Journal of Southern Medical University 2010;30(12):2640-2642
OBJECTIVETo investigate the changes in cerebral blood flow in patients with maxillary defect treated with prosthesis insertion.
METHODSThirty patients with maxillary defect receiving obturator prosthesis insertion were enrolled with another 30 subjects without dentition defect as the control. The cerebral blood flow rate was recorded before and at 5 and 10 min during mastication, and the results were analyzed statistically.
RESULTSThere was no significant difference in Vs, Vd or Vm between the two groups at the time points for measurement.
CONCLUSIONThe blood supply by the middle cerebral artery is similar between the patients receiving obturator prosthesis insertion for maxillary defect and the subjects with full denture.
Adult ; Aged ; Case-Control Studies ; Cerebrovascular Circulation ; Denture, Complete ; Female ; Humans ; Male ; Mastication ; physiology ; Maxilla ; injuries ; Maxillofacial Prosthesis ; Middle Aged ; Middle Cerebral Artery