OBJECTIVE: To establish an HPLC method for the determination of the content of nimodipine in nimodipine emulsion.METHODS: The sample was separated on Lichrospher C18 column with methanol-water-tetrahydrofuran(38∶29∶29) as the mobile phase at a flow rate of 1.3 mL?min-1.The detection wavelength was set at 237 nm;The column temperature was set at 39 ℃ and the sample size was 20 ?L.RESULTS: The linear range of nimodipine was 1.0～40.0 ?g?mL1(r=0.999 8) and the average recovery rate was 98.62%(RSD=1.28%).CONCLUSION: The established HPLC method is applicable for the determination of nimodipine content in nimodipine emulsion.

Humans ; Occupational Diseases ; prevention & control ; Occupational Health ; Stress, Psychological ; prevention & control

Burnout, Professional ; Humans

Hazardous Substances ; Risk Assessment ; methods ; Workplace

Cardiovascular Diseases ; etiology ; Humans ; Psychology, Industrial

Objective To explore the therapeutic effect of recombinant human growth hormone (rhGH) on the stature of children with Turner syndrome(TS) and analyze its influencing factors in treatment.Methods Twelve children with TS were selected in the outpatient department of West China second hospital of sichuan university between Jan.2004 to Jun.2007.They were diagnosed as TS with karyotypically.The average age was 4.9-16.9(11.28?3.64)years old.Every patient must be made sure to have normal liver function,kidney funtion and MRI for hypothalamus and pituitary before enrollment.All cases were never treated with growth hormone or estrin.Not all the patients had the same manifestations:12 cases with short stature,9 cases with cubitus valgue,8 cases with broad chest,4 cases with low posterior hairline,2 cases with webbed neck,11 cases without pubes and armpit hair,none with menarche.Every patient must be made sure to have normal liver function,kidney funtion and MRI for hypothalamus and pituitary before enrollment.rhGH was used subcutaneously 3-5 cm around navel eve-ry night before sleep.Average rhGH dose was 0.16 IU/(kg?d)[0.15-0.18 IU/(kg?d)],and height standard deviation score (HtSDS) was calculated and compared before and after rhGH treatment,and adverse reactions were observed.Results The duration of treatment was different:9 cases for 12 cases months,2 cases for 15 cases months and 1 case for 18 cases months.The average height increase rat was (0.55?0.15) cm / month.Difference of HtSDS existed before and after treatment by t test(t=2.87 P

Carbon Disulfide ; analysis ; Occupational Exposure ; prevention & control ; Threshold Limit Values ; Workplace

Adult ; Carcinoma, Hepatocellular ; secondary ; Humans ; Liver Neoplasms ; secondary ; Lymphoma, Non-Hodgkin ; pathology ; Male ; Spinal Neoplasms ; pathology

Humans ; Musculoskeletal Diseases ; epidemiology ; prevention & control ; Occupational Diseases ; epidemiology ; prevention & control