Objective To investigate the advantages and prognosis of extended full-thickness transanal local excision in treating early stage low rectal cancer.Methods Clinical gastrointestinal surgery data of transanal local excision surgeries of 163 patients from January 2003 to December 2010 was reviewed.According to the different operation modes,the patients were divided into group A (transanal local expanded full-thickness resection group,78 cases),group B (traditional open operation group,85 cases),to observe the operation status and survival time.Results The operative time [(43.17±12.31) min vs (216.41±60.12) min,t =12.924,P =0.011],the volume of bleeding (30 ml vs 110 ml,Z =-2.522,P =0.012),the postoperative intestinal function recovery time [(1.61±0.76) d vs (3.72±1.46) d,t =2.681,P =0.037),the postoperative hospital stay [(5.39±1.21) d vs (22.79±8.31) d,t =6.964,P =0.023),the rate of complication [3.85 ％ (3/78)vs 24.71％ (21/85),x2 =14.096,P ＜ 0.001] of group A and group B showed statistical significance (P ＜ 0.05),1 year,3 years,5 years survival rate of group A and group B were 100.0 ％,93.5 ％,89.5 ％ and 98.8 ％,95.2 ％,90.3 ％ respectively,the average survival time were respectively 57.08 months and 57.49 months,with no statistical difference (P ＞ 0.05).Conclusions The extended full-thickness transanal local excision in treating early stage low rectal cancer can achieve the effect of laparotomy.Compared with laparotomy,it has more advantages such as shorter operative time,less bleeding,less invasive,faster postoperative recovery,and fewer occurrences of complications.

Cardiovascular Diseases ; etiology ; Humans ; Psychology, Industrial

OBJECTIVETo observe the changes of mitochondria stress in locus coeruleus and the tyrosine hydroxylasic projection after long-term sleep deprivation.

METHODSSleep deprivation mice model was set up by employing "novel environments" method. The expression of NAD -dependent deacetylase Sirtuin type 3 (SIRT3), which regulates mitochondrial energy production and oxidative stress, and heat shock protein 60 (HSP60), a major biomarker of mitochondrial stress, and the tyrosine hydroxylasic projection from locus coeruleus were analyzed after a 5-day sleep deprivation.

RESULTSCompared to the control group, the expression of SIRT3 in locus coeruleus was significantly decreased in respouse to long-term sleep deprivation, while the expression of HSP60 was significantly increased. In addition, relative to control group, pereentage area of the tyrosine hydroxylasic projection to anterior cingulate cortex was substantial decreased in long-term sleep deprivation group.

CONCLUSIONLong-term sleep deprivation induced the decreased level of SIRT3 expression and the elevation of mitochondrial stress in locus coenileus, which may further lead to the loss of tyrosine hydroxylasic projection in mice.

Animals ; Chaperonin 60 ; metabolism ; Locus Coeruleus ; metabolism ; physiology ; Mice ; Mitochondria ; metabolism ; Mitochondrial Proteins ; metabolism ; Oxidative Stress ; physiology ; Sirtuin 3 ; metabolism ; Sleep Deprivation ; Tyrosine ; metabolism

Objective To investigate the protective effects of warm oxygenated leukocyte-depleted blood cardioplegia on myocardium during cardiac valve replacement. Methods Forty patients of both sexes undergoing elective valve replacement under CPB were randomly allocated into two groups (n = 20 each) : control group and study group. Warm blood cardioplegia was used in both groups, while in study group the leukocytes in the blood used for cardioplegia were depleted with FT-RL type leukocyte filter. The T0 of the cardioplegic solution was maintained at 32-35℃. Blood samples were taken for determination of plasma concentrations of human heart fatty acid-binding protein (HH-FABP) and malonaldehyde (MDA) and myocardial tissue was obtained from right atrium for determination of myeloperoxidase (MPO) activity before aortic cross-clamping (T0), immediately after (T1) and 30 and 60 min after aortic unclamping (T2 ,T3).Results The two groups were comparable with regard to sex, age, body weight, height, cardiac function and CPB and aortic cross-clamping time. The plasma concentrations of HH-FABP and MDA were significantly increased after aortic unclamping (T2,3) compared to the baseline (T0) in both groups but were significantly lower in study group than in control group (P

Sister chromatid exchange in myelogenous cell of mice was measured to evaluate the effects of anti- mutation by the Chinese triditional medicine , Turtle shell and tortoise shell. The results showed both of them have significant anti-mutation activites.

Objective To review our surgical treatment experience and analyze the surgical methods and efficacy of Stanford type A acute aortic dissection in elderly patients.Methods We recruited 24 patients aged over 60 years with Stanford type A acute aortic dissection treated surgically with deep hypothermic circulatory arrest and selective cerebral perfusion from June 2013 to September 2016.Depending on the patients` conditions, we selected different surgical methods, including Bentall operation in 3 cases, David operation in 1 case, partial aortic arc replacement+Bentall operation in 3 cases, total aortic arch replacement+stented elephant trunk implantation technique+Bentall operation in 12 cases, total aortic arch replacement+stented elephant trunk implantation technique+ascending aorta replacement in 5 cases, and concomitant coronary artery bypass grafting (CABG) in 3 cases.Results Surgical operation was successful in all the 24 patients.The average CPB time of the all patients was (192.9±37.1)min, the aortic cross-clamp time was (120±28.72)min, deep lower temperature of cerebral perfusion time was (36.2±11.3)min, and operation time was (544.8±91.2)min.Soon after the operation, two patients died of multiple organ dysfunction syndrome and renal failure complicated with infection, respectively.Postoperative complications included brain nerve dysfunction in 2 cases;renal insufficiency in 4 cases, which werelater treated with CRRT;pulmonary infection and tracheotomy in 3 cases;dysfunction in lower extremities in 1 case;hyoxemia in 3 cases;and incision infection in 2 cases.All the patients were followed up for 2-38 months, one patient was hospitalized because of hydropericardium and another one died of sudden death with unclear cause.Conclusion Surgical treatment of Stanford type A acute aortic dissection can obviously decrease the mortality and improve the quality of life.Total aortic arch replacement combined with transaortic stented graft implantation into the descending aorta is a classic clinical surgical treatment.Precise diagnosis, suitable surgical treatment and post-operative care are all important factors contributing to patients` recovery.

Objective To identify various arteriographic manifestations and the curative effects of emergency embolization of renal hemorrhage after percutaneous nephrolithotomy.Methods Emergency arteriography was performed in 21 patients of renal hemor-rhage after percutaneous nephrolithotomy.Emergency embolotherapy was conducted by using different embolic agents such as gelat-in sponge,polyvinyl alcohol (PVA)particles and coils after bleeding arteries were identified.The arteriographic manifestations and the curative effects of the treatment for renal hemorrhage were retrospectively analyzed.Results Contrast extravasations were con-firmed in 21 patients,among them,4 contrast extravasations with arteriovenous fistulas(AVF)of renal artery;3 pseudoaneurysms of renal artery.The hemorrhage completely stopped after emergent embolotherapy in 21 cases.During follow-up,no rebleedings and severe complications occurred.Conclusion Emergency arterial embolization for renal hemorrhage after percutaneous nephrolithotomy is a safe and effective treatment.The key to successful treatment is to select embolization agent reasonably and embolize the bleeding arteries thoroughly and completely.

Objective: To study the effects of exogenous nucleotides on immune function in mice.Methods: Sixty Kunming mice, (20?2) g, 5-6 weeks old were divided into three treatments groups (fed 0,0.05%,0.25% nucleotide-supplemented diet respectively). Each treatment was divided into two groups: normal group and immunodepression group. Mice in immunodepression groups were injected with cyclophosphamide (150 mg/kg bw) on the 2nd day in order to suppress the immune function and the mice in normal group were treated with normal saline at the same time. All mice were injected with SRBC on the 6th day. 0,0.05%,0.25% nucleotides were added into basic diet respectively and fed for 10 days. Body weight gain, organ index (thymus and spleen), SI, antibody anti SRBC and antibody secreting cell number in spleen were measured. Results: The body weight gain(P

Objective To evaluate the therapeutic effects of aminoguanidine(AG) on cerebral ischemia-reperfusion damage in rats. Methods The intravascular thread models with 2 h of occlusion and 22 h of reperfusion were made in the rats.The brain infarction size and the degree of blood brain barrier(BBB) disruption in the ischemic regions were evaluated by staining with 2,3,5-triphenyl tetrazolium chloride and observing with Evans blue fluorescence microscope.HE staining was utilized for observing neutrophil infiltration. Results The brain infarction(volume,) the area of BBB disruption and the degree of neutrophil infiltration were dramatically decreased in the treatment group as compared to the control group(P