Adult ; Aged ; Carcinoma, Renal Cell ; diagnosis ; surgery ; Disease-Free Survival ; Female ; Follow-Up Studies ; Humans ; Kidney Neoplasms ; diagnosis ; surgery ; Male ; Middle Aged ; Nephrectomy ; methods ; Treatment Outcome

Objective To study the regulatory effects of Ca 2+ , ATP, ADP and AMP on myocardial nuclear Ca 2+ ATPase activity after ischemia reperfusion injury Methods The model of myocardial ischemia reperfusion injury was established in rats Myocardial nuclei were purified using sucrose density gradient centrifugation The activity of Ca 2+ ATPase was measured with ascertaining phosphate Results Compared with the control, plasma levels of MDA and FFA after myocardial ischemia reperfusion injury increased significantly ( P

Objective Observes the sparse blood to pass treatmentacute myocardial infarction the curative effect.Meth- ods Will diagnosefor the acute myocardial infarction 62 examples patient stochasticallydivides into the treatment group and the comparison group,thetreatment group gives the sparse blood to pass with the foundationtreatment (including low molecular heparin,aspirin,Betaloc,a ACEI kind of medicine,simvastatin,tallies dissolveshitch to dissolve hitch),the comparison group will give thefoundation treatment,15 days will he a treatment course,through tothe curative effect check analysis,to will count the material tocarry on statistics processing.Results Treatment group totaleffectiveness is 93.5%,obvious is good to the foundation group(60.93%).Conclusion Adds in the traditional convention foundation- treatment with the sparse blood passes treatment acute myocardialinfarction to obtain a better curative effect.

Neural cell adhesion molecule (NCAM) is a glycoprotein expressing on the surface of neurons, glial cells, bone cells and natural killer cells. NCAM plays an important role in the process of cell - cell adhesion and cell migration, and is also a model protein to study polysialic acid. In this paper, NCAM gene from mouse mammary gland cells (NMuMG) was cloned into eukaryotic expression vectors pcDNA3.1(+) and transfected into mutant Chinese hamster ovary cells ldlD-14. The stable transfection over-expressing NCAM was obtained through the G418 selection and confirmed by Western blotting. Due to unique characters of ldlD-14 cells, carbohydrate chain of NCAM molecule can be easily manipulated with or without adding galactose in the serum free medium, and this modification can provide the basis for further studies on the effect of glycosylation on NCAM molecular function.
Animals ; CHO Cells ; Cloning, Molecular ; Cricetinae ; Cricetulus ; Female ; Galactose ; Glycosylation ; Mammary Glands, Animal ; cytology ; Mice ; Neural Cell Adhesion Molecules ; biosynthesis ; Polysaccharides ; chemistry ; Sialic Acids ; chemistry ; Transfection

Minimally Invasive Surgical Procedures ; Philosophy, Medical

OBJECTIVETo explore tentatively the impact of occupational stress on sleep disorders.

METHODSninety three male freight train dispatchers participated in this study (response rate 80.87%). Sleep disorders, occupational stressors, personalities, and occupational strain response were measured with questionnaires.

RESULTSWorkers with high psychological demands, job stressors,depressive symptoms,physical symptoms, daily life stress, work locus of control had higher sleep disorders scores than their counterparts (P < 0.01 or P < 0.05), workers with high job control had lower sleep disorders scores than their counterparts (P < 0.05). Sleep disorders were positively related to psychological demands, job stressors, depressive symptoms, physical symptoms, daily life stress, state-anxiety, strait-anxiety, and susceptibility to stress (P < 0.01 or P < 0.05), but negatively to job control and mental health (P < 0.05). Low job control, depressive symptoms, daily life stress and meaningless job were risk factors of sleep disorders.

CONCLUSIONThe sleep disorders are associated with some aspects of occupational stress among male freight train dispatchers.

Adult ; Humans ; Male ; Middle Aged ; Occupations ; Railroads ; Sleep Wake Disorders ; epidemiology ; Stress, Psychological ; Surveys and Questionnaires

Animals ; Brain-Derived Neurotrophic Factor ; metabolism ; Hypothalamus ; metabolism ; Male ; Physical Conditioning, Animal ; physiology ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo improve the method for monitoring sleep state and respiratory rhythm of SD rats, providing a solution for rats' chewing on the wires, signal loss and instability problems in the animal model of sleep apnea syndrome (SAS).

METHODSWe improved monitoring electrodes of both electrocorticogram (ECoG) and electromyogram (EMG), signal circuit and animal operation.

RESULTSOperation time was shortened and wound exposure time was reduced, which made it easier for postoperative recovery. The ECoG and EMG signals were more stable with sharp image, and signal circuit lines had better conductivity and material durability, achieving continuous monitoring for a long time and high success rate. We could precisely distinguish the sleep wake state and the sleep apnea events in rats according to these signals.

CONCLUSIONThe improved method is more reliable and practical to test the small animal model of SAS, and is more easily to operate and the signals are more stable.

Animals ; Electroencephalography ; methods ; Electromyography ; methods ; Models, Animal ; Monitoring, Physiologic ; methods ; Rats ; Respiration ; Sleep ; Sleep Apnea Syndromes ; diagnosis

Emergency medicine and critical care medicine are the new contents in standardized training of residency.The system of standardized training residency lacks for a traditional way to refer.According to the characteristic of the subjects and the practical situation,this paper discusses the methods of training in studying basic theory,idea education,improving technology and strain capability on improving the capability of standardized training residency in internal intensive medicine.

Aim To establish a model of the vascular endothelial cell (VEC) injury in SDrats.Methods SD rats were randomly divided into the control and the modelgroups. The model rats were injected with adrenaline diluted to 2. 5 times 0. 05 mg?100 g-1 (tid) for 5 d continously. From the 4th d, they were irritated for 5 min in the0℃ cold-water in the middle between adrenaline injections.The control rats weregiven 0. 9% NS as above. At 6th d, blood samples were taken from carotid arteries ofthe rats and the CEC counts, t - PA、PAI activities, 6-keto-PGF1? concentrations andthe platelet aggregation rate(max) were detected respectively. Results In the modelgroup, as compared with those in the control group, t - PA activity and 6-keto-PGF1?concentration decreased significantly(P