1.Real-time quantifi cation for BCR-ABL transcripts in chronic myeloid leukaemia patients in UKMMC, Malaysia
FL Wong ; NH Hamidah ; AA Hawa ; AN Nurul ; CF Leong ; SAW Fadilah ; O Ainoon
The Malaysian Journal of Pathology 2011;33(2):107-112
Molecular pathogenesis of chronic myeloid leukemia (CML) is well established and molecular
monitoring for patients with CML has become an important practice in the management of patients
on imatinib therapy. In the present study, we report the use of RQ-PCR method for detection of
BCR-ABL fusion gene for our CML cases. We performed a two-step RQ-PCR on bone marrow
aspirates or peripheral blood of 37 CML patients. Quantitative expression of BCR-ABL fusion
gene was carried out relative to the expression of a housekeeping gene as endogenous control to
compensate for uneven cell numbers, RNA quality, or variations in reverse transcription effi ciencies.
Twenty-four of these patients were pre-treated with hydroxyurea or alpha interferon prior to the
imatinib therapy. Their BCR-ABL fusion gene levels were monitored for 18 months. All samples
processed were evaluable. The PCR amplifi cation effi ciency of the ABL gene is 90.5% (0.2158)
and the BCR-ABL gene, 93.4% (0.1573).
2.Radiographic features of plasma cell leukemia in the maxilla: A case report.
Phillip WONG ; Deeba KASHTWARI ; Madhu K NAIR
Imaging Science in Dentistry 2016;46(4):273-278
Plasma cell leukemia (PCL) is an aggressive form of multiple myeloma where there is hematogenous spread of abnormal plasma cells into the periphery. This is opposed to multiple myeloma, where the abnormal plasma cells stay in the bone marrow. PCL is more common in males than females, and is also more common in African-Americans than Caucasians. Signs and symptoms of PCL include, but are not limited to, renal insufficiency, hypercalcemia, anemia, lytic bone lesions, thrombocytopenia, hepatomegaly, and splenomegaly. Here, we discussed a case of a 71-year-old Caucasian female recently diagnosed with primary PCL with radiographic features of this disease throughout the body, with an emphasis on the maxillofacial skeleton and relevance from a dental standpoint.
Aged
;
Anemia
;
Bone Marrow
;
Female
;
Hepatomegaly
;
Humans
;
Hypercalcemia
;
Leukemia, Plasma Cell*
;
Male
;
Maxilla*
;
Multiple Myeloma
;
Pathology
;
Plasma Cells*
;
Plasma*
;
Renal Insufficiency
;
Skeleton
;
Splenomegaly
;
Thrombocytopenia