OBJECTIVE:To study the metabolisms of calcium and magnesium in blood plasma and akaryocyte of patients with pregnancy-induced hypertension before and after treatment with magnesium sulfate.METHODS:The concentrations calcium and magnesium in plasma and akaryocyte of healthy women,healthy pregnant women and patients with pregnancy-induced hypertension syndrome were determined by atomic absorption spectrophotometry.RESULTS:Plasma low calcium,high calcium level and low magnesium level in erythrocytes were found in patients with pregnancy-induced hypertension syndrome,which had improvements after treatment with magnesium sulfate.CONCLUSION:There is abnormalism of calci?um and magnesium metabolism in patient with pregnancy-induced hypertension syndrome,which may improved after treat?ment with magnesium sulfate,but the dosage of which should be individualized.

The article analyzes the implementation of the new hospital financial and accounting principle in the hospi-tal financial management and accounting practice, in order to provide some reference for perfecting hospital accounting system construction.

Objective:To clarify the financial revenue and expenditure accounting elements in hospitals.Methods:According to the hospital accounting principle,summarize the whole business process of fiscal indicators issued,revenue recognition,actual payment,reconciliation and confirmation in public hospitals.Results and Conclusion:Cases and suggestions were proposed to standardize and improve the hospital financial revenue and expenditure accounting.

Objective: To reasonably adjust the medical prices on the basis of cancelling drug prices policies. Methods: Taking the data from sample hospitals in Guangdong as example, to discuss how to establish model of medical service item pricing scientifically and reasonably. Results:The model of medical service item pricing has been established and practical care has been estimated. Conclusion:It needs to adjust the charging of medical technical services to achieve the prospective effects of medical reform.

A fine performed coronary artery stent is characterized by excellent biocompatibility, proper expansion ratio, adequate flexibility and complaisance, easy to sterilization, and controllable microenvironment, which can promote the growth of adhered cells. The main question need to be considered in coronary artery stent application is blood and tissue compatibility. Coronary artery stent bring a hope for patients with coronary artery disease by inhibiting intimal hyperplasia, and decreasing occurrence of dangerous complication. However, as a kind of foreign body, the stent implantation is closely associated with complication, such as ventricular fibrillation, acute vascular occlusion, side-branch occlusion, vessel dissection, coronary artery spasm or perforation, vasovagal reflex and immune reaction. Therefore, various complications should attract more attention in treating coronary artery disease with stent implantation.

Hepatic veno-occlusive disease (HVOD) is a serious complication of hematopoietic stem cell transplantation.The mortality rate of severe HVOD (sHVOD) approaches 100 ％.Defibrotide (DF) is a polydisperse mixture of single-stranded oligonucleotides with antithrombotic and fibrinolytic properties.Numerous clinical trials have demonstrated its effectiveness and safety in the prevention and treatment of HVOD.This review focuses on the possible mechanisms of action of DF and its use in the prevention and treatment of HVOD.

Objective To study the effect of hydroxychloroquine (HCQ) on the suppressors of cytokine signaling (SOCS) in the kidney of MRL/lpr mice and evaluate the protection of HCQ on lupus nephritis.Methods Twenty-six rats were randomly divided into 3 groups:the control group which was consisted of 6 mice; the MRL/lpr group which was consisted of 10 mice; and the HCQ group which was consisted of 10.24-hour urinary protein level and anti dsDNA levels were tested.The expressions of SOCS-1 and SOCS-3 protein were determined by western blot.Analysis of variance was used to determine statistically significant differences between the two groups.A P value less than 0.05 was considered to be statistically significant.Results Compared with the control group,the levels of 24-hour urinary protein [(2 356.6±509.3) mg vs (440.3±90.1)mg] and anti ds-DNA (128.7±32.3 vs 14.9±1.4) were significantly increased in the MRL/lpr group,the expression levels of SOCS-1 and SOCS-3 protein were increased.After treated with HCQ,the levels of 24-hour urinary protein [(70.8±11.1) mg vs (2 356.6±509.3) mg] and anti-dsDNA (111.8±330.1 vs 128.7±32.3)were significantly decreased,the expression level of SOCS-1 and SOCS-3 protein decreased significantly (P＜ 0.05).Conclusion HCQ can inhibit SOCS expression in the kidney of lupus mice,reduce proteinuria and delay progression of immune disorders and lupus nephritis.

0.05). Conclusion The results suggest that there is autoimmunological mechani sm in the pathogenesis of psoriasis.

Objective To study the effect of caveolin-1 on renal injury and the expression of tight junction proteins in MRL/lpr mice kidney.Methods The mice were divided into 4 groups:5 mice in the normal control group (BALB/c mice);the MRL/lpr lupus mice (n=18) were randomly divided into the MRL/lpr group in which 6 mice were included;the negative control group in which 6 mice were included;the caveolin1 transfection group in which 6 mice were included.The changes of urine protein,the levels of urea (BUN) and creatinine (Cr) were detected.The expressions of claudin-5,occludin,ZO-1 and caveolin-1 protein were determined by western bloting.Analysis of variance was used to determine statistical significant differences between the two groups.A significance level of 0.05 was considered as signigicant.Results Compared with the control group,24 h urine protein [(2 894±437) mg,(412±72) mg],BUN [(8.7±1.5) mmol/L,(6.9±0.4) mmol/L],Cr [(106±22) μmol/L,(85±4) μmol/L] were significantly increased,level of caveolin-1 protein increased (265±17,61±6),the level of occludin (114±12,190±12),claudin-5 (60±5,80±6) and ZO-1 (98±11,206±15) protein decreased in the MRL/lpr group (P＜0.05).After caveolin-1 transfection,the levels of urinary protein [(1 253±249) mg,(2 894±437) mg],BUN [(6.5±1.3) mmol/L,(8.7±1.5) mmol/L],Cr [(78±17) μmol/L,(106±22)μmol/L] were significantly decreased,and the levels of occludin (218±16,114±12),claudin-5 (87±6,60±5)ZO-1 (313±17,98±11) were increased (P＜0.05).Conclusion The expression of caveolin-1 protein in the renal tissues of lupus nephritis increases.Caveolin-1 can reduce the expression of tight junction proteins and contribute to progres-sion of lupus nephritis.

Objective To determine the level of anticyclophilin A auto-antibodies (ACA) in sera of patients with psoriasis. Methods Cyclophilin A (CyPA) was extracted and purified from bovine thymus. The level of anticyclophilin A auto-antibodies (ACA) was detected by enzyme-linked immunosorbent assay (ELISA) and immunoblotting test (IBT) in sera of patients with psoriasis. Results It was shown that the level of ACA was significantly higher in the sera of patients with psoriasis than those in control group (IBT:P<0.01,ELISA:P< 0.01). ACA was found to be positive in 41.3％ of the patients by ELISA. In psoriatic group, the serum level of ACA was higher during the progressive stage than that in the stationary stage, however, no significant difference was observed (IBT:P>0.05,ELISA:P >0.05). Conclusion The results suggest that there is autoimmunological mechanism in the pathogenesis of psoriasis.