Animals ; Exobiology ; methods ; Extraterrestrial Environment ; Genomics ; Humans ; Space Flight

OBJECTIVETo substantiate the effects of spaceflight on the glycyrrhizic acid-related gene mutation in Glycyrrhiza uralensis.

METHODLicorice (G. uralensis) seeds were carried by a recoverable satellite for 18 days (the average radiation dose in the flight recovery module was 0. 102 m x d(-1), the orbit semidiameter 350 km, gravity 10(-6)). After returned to the earth, the satellite-flown seeds and the un-flown seeds (ground control) were planted in the fields of experimental farm. The leaves of each group were used for studying the effects of space flight on the glycyrrhizic acid-related gene mutation including ITS sequence and beta-amyrine synthase gene.

RESULTThe ITS sequence of glycyrrhizic acid related gene showed no changes after spaceflight. While beta-amyrine synthase gene had some different points after spaceflight and the different points could get the expression.

CONCLUSIONThe results indicated that spaceflight induce genetic variation in G. uralensis and spaceflight could also have effects on glycyrrhizic acid-related gene mutation in G. uralensis. It may need to further research how the spaceflight induced the mutation of the glycyrrhizic acid related gene. The results suggested that recoverable satellite-flown condition could bring inheritable mutagenic effects on G. uralensis seeds and maybe used as a tool for accelerating the progress in G. uralensis breeding.

Extraterrestrial Environment ; Glycyrrhiza uralensis ; genetics ; metabolism ; Glycyrrhizic Acid ; metabolism ; Mutation ; Plant Proteins ; genetics ; metabolism ; Space Flight

OBJECTIVEIn order to find the new varieties with different horticultural characters, and investigate the mutation effects of seeds of Dioscorea zingerbrensis.

METHODThe seeds were carried by a satellite into space and recovered. The space mutation effect on the germination, seedling growth, chromosomes and rhizome diosgenin content of SP, populations of D. zingerbrensis were investigated.

RESULTStimulated by space environment, the seed possessed the fast germinating characteristics. Germination rate showed no change. It was also found that a few plants were aneuploid or tetraploid. Fresh rhizome weight and rhizome diosgenin content in the second year plant were remarkably higher than those of the control. However, the increasing of third year plant was slow down, and rhizome diosgenin content in the third year plant declined simultaneously.

CONCLUSIONThe space environment showed stimulating effects on seed germination, fresh rhizome weight and rhizome diosgenin content.

Dioscorea ; chemistry ; genetics ; physiology ; Extraterrestrial Environment ; Germination ; Mutation ; Plant Extracts ; chemistry ; Seedlings ; chemistry ; genetics ; physiology

OBJECTIVETo investigate the space environment on the role of licorice mutagenesis analysis of proteins.

METHODLicorice (Glycyrrhiza uralensis) seeds were carried by a recoverable satellite for 18 days (the average radiation dose in the flight recovery module was 0.102 m x d(-1), the orbit semidiameter 350 km, gravity 10(-6)). After return, The satellite-flown seeds and the unflown seeds (ground control) were planted in the fields of experimental farm. The leaves of each group were used for studying the effects of space flight on CAT, SOD activity, the protein content and electrophoresis.

RESULTAfter the space flight, CAT, SOD activity of licorice increased in varying degrees, the difference was significant (P<0.05), two types of enzyme activity of sample from Ordos were higher than that from Hangjinqi. The protein content of licorice increased in a certain extent, the difference was significant (P<0.05), while protein electrophoresis also showed differences, weak new bands appeared.

CONCLUSIONThese results indicated that spaceflight has effect on protein of licorice, these changes may be used as a tool for accelerating the progress in G. uralensis breeding.

Chloramphenicol O-Acetyltransferase ; analysis ; metabolism ; Electrophoresis ; Extraterrestrial Environment ; Glycyrrhiza uralensis ; chemistry ; enzymology ; Plant Proteins ; analysis ; metabolism ; Spacecraft ; Superoxide Dismutase ; analysis ; metabolism

To further study the characteristics of changes on the molecular level of rice mutants induced in space environment, we analyzed proteins in leaves and seeds of four rice mutants (two high-tillering and two low-tillering) in the 8(th) and 9(th) generations after a 15-day spaceflight, and compared with their ground controls by two-dimentional polyacrylamide gel electrophoresis and reverse phase liquid chromatography (RPLC). In addition, the albumin, globulin, prolamine, glutelin, and amylose of the mutant seeds were analyzed by RPLC and ultra-violet spectrometry. The results showed that the low-abundance proteins of leaves in the peak tillering stage are more likely to be induced compared with their corresponding controls. The albumin, globulin, and prolamine of the mutant seeds revealed changes when compared with their controls, and the characteristics of changes in different mutants were stably inherited in the 8(th) and 9(th) generations, suggesting that they can be used as bio markers to identity the mutants induced by spaceflight. Moreover, two proteins (SSP9111 and SSP6302) were found to be expressed with high intensity (two-fold change) in different mutants, which were both correlated with photosystem according to mass spectrometry and database searching.
Albumins ; genetics ; metabolism ; Amylose ; genetics ; metabolism ; Chromatography, Liquid ; Electrophoresis, Gel, Two-Dimensional ; Extraterrestrial Environment ; Gene Expression Regulation, Plant ; Genetic Variation ; Genomic Instability ; Globulins ; genetics ; metabolism ; Mass Spectrometry ; Mutation ; Oryza ; genetics ; metabolism ; Plant Leaves ; genetics ; Plant Proteins ; genetics ; metabolism ; Prolamins ; Seeds ; genetics ; Space Flight