1.Mechanisms of Extracellular Vesicles Involved in Multiple Myeloma --Review.
Yi-Hui GUO ; Jia-Wei XU ; Hui SONG ; Qing ZENG ; Wei-Min CHENG
Journal of Experimental Hematology 2022;30(5):1612-1616
Multiple myeloma (MM) is a common hematologic tumor characterized by malignant proliferation of clonal plasma cells, the exact pathogenesis of which is not yet fully understood. The extracellular vesicles (EV) are structures released by cells into their surroundings that do not have a functional nucleus and can communicate between cells or deliver biologically active proteins and nucleic acids to target cells. EV play an important role in the interaction between myeloma cells and the bone marrow microenvironment, and they can promote MM progression. In this paper, we summarize the recent research progress in the mechanism of action of EV on MM in order to provide inspiration for exploring new strategies for MM treatment and prognostic stratification.
Bone Marrow/metabolism*
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Extracellular Vesicles/pathology*
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Hematologic Neoplasms/metabolism*
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Humans
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Multiple Myeloma/pathology*
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Nucleic Acids/metabolism*
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Tumor Microenvironment
2.New insight into inter-organ crosstalk contributing to the pathogenesis of non-alcoholic fatty liver disease (NAFLD).
Xu ZHANG ; Xuetao JI ; Qian WANG ; John Zhong LI
Protein & Cell 2018;9(2):164-177
Non-alcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver dysfunction and a significant global health problem with substantial rise in prevalence over the last decades. It is becoming increasingly clear that NALFD is not only predominantly a hepatic manifestation of metabolic syndrome, but also involves extra-hepatic organs and regulatory pathways. Therapeutic options are limited for the treatment of NAFLD. Accordingly, a better understanding of the pathogenesis of NAFLD is critical for gaining new insight into the regulatory network of NAFLD and for identifying new targets for the prevention and treatment of NAFLD. In this review, we emphasize on the current understanding of the inter-organ crosstalk between the liver and peripheral organs that contributing to the pathogenesis of NAFLD.
Adipose Tissue
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pathology
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Animals
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Extracellular Vesicles
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metabolism
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Humans
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Hypothalamus
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metabolism
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Intestines
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microbiology
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pathology
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Non-alcoholic Fatty Liver Disease
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etiology
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metabolism
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microbiology
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pathology
3.Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.
Weilian LIANG ; Yang CHEN ; Hanzhe LIU ; Hui ZHAO ; Tingting LUO ; Hokeung TANG ; Xiaocheng ZHOU ; Erhui JIANG ; Zhe SHAO ; Ke LIU ; Zhengjun SHANG
International Journal of Oral Science 2022;14(1):36-36
Tumor volume increases continuously in the advanced stage, and aside from the self-renewal of tumor cells, whether the oncogenic transformation of surrounding normal cells is involved in this process is currently unclear. Here, we show that oral squamous cell carcinoma (OSCC)-derived small extracellular vesicles (sEVs) promote the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of normal epithelial cells but delay their apoptosis. In addition, nuclear-cytoplasmic invaginations and multiple nucleoli are observed in sEV-treated normal cells, both of which are typical characteristics of premalignant lesions of OSCC. Mechanistically, miR-let-7c in OSCC-derived sEVs is transferred to normal epithelial cells, leading to the transcriptional inhibition of p53 and inactivation of the p53/PTEN pathway. In summary, we demonstrate that OSCC-derived sEVs promote the precancerous transformation of normal epithelial cells, in which the miR-let-7c/p53/PTEN pathway plays an important role. Our findings reveal that cancer cells can corrupt normal epithelial cells through sEVs, which provides new insight into the progression of OSCC.
Carcinoma, Squamous Cell/pathology*
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Cell Line, Tumor
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Cell Movement
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Cell Proliferation
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Cell Transformation, Neoplastic
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Down-Regulation
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Epithelial Cells/metabolism*
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Extracellular Vesicles/pathology*
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Humans
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MicroRNAs/metabolism*
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Mouth Neoplasms/pathology*
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PTEN Phosphohydrolase/metabolism*
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Tumor Suppressor Protein p53/metabolism*
4.Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressive FAT1 and inhibits stemness maintenance in thyroid carcinoma.
Jie SHAO ; Wenjuan WANG ; Baorui TAO ; Zihao CAI ; Haixia LI ; Jinhong CHEN
Frontiers of Medicine 2023;17(6):1186-1203
Through bioinformatics predictions, we identified that GTF2I and FAT1 were downregulated in thyroid carcinoma (TC). Further, Pearson's correlation coefficient revealed a positive correlation between GTF2I expression and FAT1 expression. Therefore, we selected them for this present study, where the effects of bone marrow mesenchymal stem cell-derived EVs (BMSDs-EVs) enriched with GTF2I were evaluated on the epithelial-to-mesenchymal transition (EMT) and stemness maintenance in TC. The under-expression of GTF2I and FAT1 was validated in TC cell lines. Ectopically expressed GTF2I and FAT1 were found to augment malignant phenotypes of TC cells, EMT, and stemness maintenance. Mechanistic studies revealed that GTF2I bound to the promoter region of FAT1 and consequently upregulated its expression. MSC-EVs could shuttle GTF2I into TPC-1 cells, where GTF2I inhibited TC malignant phenotypes, EMT, and stemness maintenance by increasing the expression of FAT1 and facilitating the FAT1-mediated CDK4/FOXM1 downregulation. In vivo experiments confirmed that silencing of GTF2I accelerated tumor growth in nude mice. Taken together, our work suggests that GTF2I transferred by MSC-EVs confer antioncogenic effects through the FAT1/CDK4/FOXM1 axis and may be used as a promising biomarker for TC treatment.
Mice
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Animals
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Cell Line, Tumor
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Cell Proliferation
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Mice, Nude
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Epithelial-Mesenchymal Transition
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Thyroid Neoplasms/pathology*
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Extracellular Vesicles/pathology*
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Mesenchymal Stem Cells
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Transcription Factors, TFIII/metabolism*
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Neoplastic Stem Cells/pathology*
5.Carcinoma-associated fibroblast-derived lysyl oxidase-rich extracellular vesicles mediate collagen crosslinking and promote epithelial-mesenchymal transition via p-FAK/p-paxillin/YAP signaling.
Xue LIU ; Jiao LI ; Xuesong YANG ; Xiaojie LI ; Jing KONG ; Dongyuan QI ; Fuyin ZHANG ; Bo SUN ; Yuehua LIU ; Tingjiao LIU
International Journal of Oral Science 2023;15(1):32-32
Carcinoma-associated fibroblasts (CAFs) are the main cellular components of the tumor microenvironment and promote cancer progression by modifying the extracellular matrix (ECM). The tumor-associated ECM is characterized by collagen crosslinking catalyzed by lysyl oxidase (LOX). Small extracellular vesicles (sEVs) mediate cell-cell communication. However, the interactions between sEVs and the ECM remain unclear. Here, we demonstrated that sEVs released from oral squamous cell carcinoma (OSCC)-derived CAFs induce collagen crosslinking, thereby promoting epithelial-mesenchymal transition (EMT). CAF sEVs preferably bound to the ECM rather than being taken up by fibroblasts and induced collagen crosslinking, and a LOX inhibitor or blocking antibody suppressed this effect. Active LOX (αLOX), but not the LOX precursor, was enriched in CAF sEVs and interacted with periostin, fibronectin, and bone morphogenetic protein-1 on the surface of sEVs. CAF sEV-associated integrin α2β1 mediated the binding of CAF sEVs to collagen I, and blocking integrin α2β1 inhibited collagen crosslinking by interfering with CAF sEV binding to collagen I. CAF sEV-induced collagen crosslinking promoted the EMT of OSCC through FAK/paxillin/YAP pathway. Taken together, these findings reveal a novel role of CAF sEVs in tumor ECM remodeling, suggesting a critical mechanism for CAF-induced EMT of cancer cells.
Humans
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Paxillin/metabolism*
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Protein-Lysine 6-Oxidase/metabolism*
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Carcinoma, Squamous Cell/pathology*
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Epithelial-Mesenchymal Transition
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Integrin alpha2beta1/metabolism*
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Mouth Neoplasms/pathology*
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Collagen/metabolism*
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Fibroblasts
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Extracellular Vesicles/metabolism*
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Cell Line, Tumor
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Tumor Microenvironment
6.Extracellular Vesicles in Neurodegenerative Diseases: A Double-Edged Sword.
Tissue Engineering and Regenerative Medicine 2017;14(6):667-678
Extracellular vesicles (EVs), a heterogenous group of membrane-bound particles, are virtually secreted by all cells and play important roles in cell-cell communication. Loaded with proteins, mRNAs, non-coding RNAs and membrane lipids from their donor cells, these vesicles participate in normal physiological and pathogenic processes. In addition, these subcellular vesicles are implicated in the progression of neurodegenerative disorders. Accumulating evidence suggests that intercellular communication via EVs is responsible for the propagation of key pathogenic proteins involved in the pathogenesis of amyotrophic lateral sclerosis, Parkinson's diseases, Alzheimer's diseases and other neurodegenerative disorders. For therapeutic perspective, EVs present advantage over other synthetic drug delivery systems or cell therapy; ability to cross biological barriers including blood brain barrier (BBB), ability to modulate inflammation and immune responses, stability and longer biodistribution with lack of tumorigenicity. In this review, we summarized the current state of EV research in central nervous system in terms of their values in diagnosis, disease pathology and therapeutic applications.
Amyotrophic Lateral Sclerosis
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Blood-Brain Barrier
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Cell- and Tissue-Based Therapy
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Central Nervous System
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Diagnosis
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Drug Delivery Systems
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Extracellular Vesicles*
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Humans
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Inflammation
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Membrane Lipids
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Neurodegenerative Diseases*
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Pathology
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RNA, Messenger
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RNA, Untranslated
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Tissue Donors