Adolescent ; Child ; Cleft Palate ; pathology ; Eustachian Tube ; anatomy & histology ; pathology ; Female ; Humans ; Male ; Nasopharynx ; anatomy & histology ; pathology ; Young Adult

The purpose of this study was to describe the luminal development of the murine eustachian tube and middle ear. Thirty specimens, aging from gestational day 11 to postnatal day 21, were investigated through the light microscopic observations. The present study also used digitizer, computer, and serially sectioned temporal bone specimens for three-dimensional reconstruction to measure the volume of the eustachian tube and middle ear cavity at different gestational and postnatal ages. The first pharyngeal pouch elongated during gestational day 12 to form the tubotympanic recess. Between gestational day 13 and 14 this tubotympanic recess extended to the middle ear area. A rapid increment in the volume of the tubotympanic recess was noted between gestational day 15 and 16. At this age, a definite division of the tubotympanic recess into the eustachian tube and middle ear cavity was observed. During the postnatal period, the maximum change of the middle ear volume was noted on postnatal day 11 when the mesenchymal tissue in the middle ear cavity disappeared completely.
Animal ; Ear, Middle/anatomy & histology/*embryology/growth & development ; Eustachian Tube/anatomy & histology/*embryology/growth & development ; Female ; Male ; Mice ; Mice, Inbred BALB C ; Pregnancy

We report the application of optical coherence tomography (OCT) to the diagnosis and evaluation of otitis media (OM). Whereas conventional diagnostic modalities for OM, including standard and pneumatic otoscopy, are limited to visualizing the surface of the tympanic membrane (TM), OCT effectively reveals the depth-resolved microstructure below the TM with very high spatial resolution, with the potential advantage of its use for diagnosing different types of OM. We examined the use of 840-nm spectral domain-OCT (SD-OCT) clinically, using normal ears and ears with the adhesive and effusion types of OM. Specific features were identified in two-dimensional OCT images of abnormal TMs, compared to images of healthy TMs. Analysis of the A-scan (axial depth scan) identified unique patterns of constituents within the effusions. The OCT images could not only be used to construct a database for the diagnosis and classification of OM but OCT might also represent an upgrade over current otoscopy techniques.
Adult ; Ear Canal/anatomy & histology ; Eustachian Tube/physiology ; Female ; Humans ; Male ; Middle Aged ; Otitis Media/classification/*diagnosis ; Tomography, Optical Coherence/instrumentation/*methods ; Tympanic Membrane/*physiology

We report the application of optical coherence tomography (OCT) to the diagnosis and evaluation of otitis media (OM). Whereas conventional diagnostic modalities for OM, including standard and pneumatic otoscopy, are limited to visualizing the surface of the tympanic membrane (TM), OCT effectively reveals the depth-resolved microstructure below the TM with very high spatial resolution, with the potential advantage of its use for diagnosing different types of OM. We examined the use of 840-nm spectral domain-OCT (SD-OCT) clinically, using normal ears and ears with the adhesive and effusion types of OM. Specific features were identified in two-dimensional OCT images of abnormal TMs, compared to images of healthy TMs. Analysis of the A-scan (axial depth scan) identified unique patterns of constituents within the effusions. The OCT images could not only be used to construct a database for the diagnosis and classification of OM but OCT might also represent an upgrade over current otoscopy techniques.
Adult ; Ear Canal/anatomy & histology ; Eustachian Tube/physiology ; Female ; Humans ; Male ; Middle Aged ; Otitis Media/classification/*diagnosis ; Tomography, Optical Coherence/instrumentation/*methods ; Tympanic Membrane/*physiology

OBJECTIVE: To observe the morphological alterations in pharyngeal ostium of Eustachian tube and its surrounding tissues and its correlation with secretory otitis media. METHOD: 72 volunteers (144 ears) as control group, 89 patients (151 ears) as SOM group, all were observed for the shape of pharyngeal opening of auditory tube and the morphological changes of its surrounding structures. RESULT: Statistic difference was found between the shape of pharyngeal ostium of eustachian tube of adult patients with SOM and that of control group, but for children, there was no difference. It indicated that there was a difference between adult and children in morphological changes of pharyngeal ostium of eustachian tube when suffered from SOM. CONCLUSION Morphological changes of pharyngeal ostium of eustachian tube play an important role in the genesis and development of adult SOM . The primary change in children patients is the inflammation of nasopharynx, then is the hypertrophy of adenoid and tubal tonsil. Nasopharyngeal-fibroscope is a very useful tool in the diagnosis and treatment of SOM.
Adolescent ; Adult ; Case-Control Studies ; Child ; Child, Preschool ; Eustachian Tube ; anatomy & histology ; pathology ; Humans ; Middle Aged ; Otitis Media with Effusion ; pathology ; Young Adult