Adeno-associated virus (AAV) and human papillomavirus (HPV) DNAs were found in abnormal quality semen, early abortus and female genital tissues. It was suggested that they might cause male infertility and miscarriages. This study was performed to determine the detection rate of these viruses in the semen and to assess the relationship between the presence of virus and male factor infertility and recurrent miscarriages. Sixty-three of 99 recruited male were included in this study according to the completeness of follow-up and the sample availability. Fourteen male with normal reproductive capacity were allocated to control group, 15 male with abnormal results in semen analysis were grouped as male factor infertility (MF) group, and 34 male whose spouses have had history of repeated spontaneous abortions were designated as repeated miscarriage (RM) group. AAV and HPV were detected in semen by polymerase chain reaction. The detection rate of AAV in the MF infertility group and RM group was 60.0% and 50.0%, respectively, while 14.3% in the control group (p < 0.05). However, the differences in the detection rate of HPV were not statistically significant among groups. These results suggest that AAV could be related to repeated miscarriages and male infertility.
Abortion, Habitual ; Abortion, Spontaneous ; Dependovirus ; DNA ; Female ; Fertilization ; Follow-Up Studies ; Humans ; Infertility ; Infertility, Male ; Male ; Polymerase Chain Reaction ; Pregnancy ; Semen ; Semen Analysis ; Spouses ; Stress, Psychological ; Viruses

PURPOSE: The purpose of this study was to evaluate the usefulness of contrast enhancement in assessing thedepth of stromal invasion in patients with uterine cervical tumors by comparing dynamic and late contrast-enhancedT1-weighted MR imaging with T2-weighted MR imaging. MATERIALS AND METHODS: Of 58 surgically proven uterinecervical cancer patients, 31 in whom tumors were seen on MRI were included in this study. Using a 1.5 T magnet,T2-weighted contrast-enhanced dynamic, and T1-weighted MR imaging were performed. In each MR imaging sequence,tumor visualization, margin delineation, enhancement pattern and depth of stromal invasion were evaluated onsagittal images and were correlated with pathological findings of resected uterus, focusing on the depth ofstromal invasion. RESULTS: Surgical FIGO stages were IB1 in 20 patients, IB2 in three, IIA in six, and IIB intwo. A tumor was detected in 29 (94%) patients on T2-weighted images, in 26 (84%) on dynamic contrast enhancedimages, and in 28 (90%) on contrast enhanced T1-weighted images. The tumor demonstrated a clear margin in 22 (71%)patients on late contrast-enhanced T1-weighted images, in 21 (68%) on dynamic images, and in 13 (42%) onT2-weighted images. Correlated with pathologic findings, the depth of stromal invasion was overestimated onT2-weighted images in eight (26%) patients, and on dynamic and late contrast-enhanced T1-weighted images in three(10%) and three (10%) respectively. CONCLUSION: Tumor margins are clearer and the extent of tumors may be moreaccurately evaluated on dynamic MR and late contrast-enhanced T1-weighted imaging. These sequences thas seem to beuseful, and superior to T2-weighted imaging, for assessing the depth of stromal invasion in patients with cervicalcarcinoma.
Cervix Uteri* ; Female ; Humans ; Magnetic Resonance Imaging* ; Uterus

OBJECTIVE: The objective of this study was to compare the outcomes of cryopreserved-thawed blastocyst transfer (CT-BT) in natural or programmed cycles using exogenous steroid hormones. METHODS: A total of 221 CT-BT cycles were included and divided into two groups according to endometrial preparation protocols. In natural cycle group (n=116), monitoring was performed by transvaginal ultrasonography to detect ovulation. In programmed cycle group (n=105), oral estradiol valerate, 6 mg/day, was started on the third day of the menstrual cycle and administered continuously, and progesterone in oil 100 mg i.m. daily injection was started on cycle day 15. CT-BTs were performed on five days after ovulation in natural cycles and five days after the initiation of progesterone administration in programmed cycles. Pregnancy rates, implantation rates, and other clinical characteristics of the two groups were compared. RESULTS: Clinical characteristics of study subjects did not differ between the two groups. Post-thaw survival rates, number of transferred blastocysts, and number of good-quality blastocysts were not different. There were no statistically significant differences in implantation rates (21.1% vs. 19.4%), clinical pregnancy rates (36.2% vs. 36.2%), and ongoing pregnancy rates (28.4% vs. 27.6%) between the two groups. CONCLUSIONS: No statistically significant differences were found in pregnancy rates and implantation rates between the two protocols. Our results suggest that both protocols are equally effective for endometrial preparation in CT-BT cycles.
Blastocyst* ; Embryo Transfer* ; Estradiol ; Female ; Menstrual Cycle ; Ovulation ; Pregnancy Rate ; Progesterone ; Survival Rate ; Ultrasonography