No abstract available.
Reoperation*

Calcium has been implicated as a primary pathogenetic mediator of cellular injury under conditions of oxygen and substrate deprivation in the kidney aswell as other tissues. It has been proposed that calcium entry from the external medium increases intracellular free calcium to toxic levels there-by converting cellular injury from a potentially reversible to an irreversible state. Cellular calcium homeostasis is a fundamental prerequisite for many coordinate cellular functions. The regulation of cellular Ca++ -metabolism is maintained mainly by Ca++ -ATPase. The purpose of the present study was to determine the effects of ischemia on the subcellular specific activity of Ca++ -ATPase of renal tissues in the rabbits. 1. In both normal cortical and medullary tissues, the specific activities Ca++ -ATPase were higher in mitochondria than in cytosol. 2. In cortical tissue, the specific activities of mitochondrial Ca++ -ATPase were increased with duration of ischemic time under normothermia. But the specific activities were decreased comparing to those of control under hypothermia, and the activities were not different with those of control in Verapamil treated group. 3. The specific activities of Ca++ -ATPase of cortical cytosol were increased as in mitochondria under normothermia. And the activities were similar with those of control in hypothermic and Verapamil treated groups. 4. In medullary tissues, the specific activities of mitochondrial Ca++ -ATPase were increased with duration of ischemic time under normothermia. In Verapamil treated group, the activities were decreased comparing to those of control. And there was no difference of activities between hypothermia and control groups. 5. As in cortical tissues, the specific activities of Ca++ -ATPase of medullary, cytosol were increased in normothermic group. And the activities were similar with those of control in hypothermic and Verapamil treated groups. Above results suggested that calcium contents of mitochondria and cytosol were increased with duration of ischemic time under normothermia. Hypothermia and Verapamil treatment seemed to be beneficial effects in prevention of calcium accumulation in the renal tissues.
Adenosine Triphosphatases* ; Calcium ; Cytosol ; Homeostasis ; Hypothermia ; Ischemia* ; Kidney ; Mitochondria ; Oxygen ; Rabbits* ; Subcellular Fractions* ; Verapamil

No abstract available.
Cystic Adenomatoid Malformation of Lung, Congenital* ; Lung*

No abstract available.
Leiomyoma*

Phytases (myo-inositol hexakisphosphate phosphohydrolase; EC 3.1.3.8) are enzymes which catalyze the hydrolisys of phytate into myo-inositol and inorganic phosphates. Phytases are found in plants and a variety of microorganisms. Aspergillus species were treated with 254 nm of UV irradiation for the screening of phytase overproducing mutant strains. At 15 minute irradiation, the survivals of population were less than 5%, and UV irradiation time was decided at 20 minute for the isolation of mutant strains. Four UV mutant strains in A. oryzae (YUV-47, -169, -341, -511) and six in A. ficuum (FUV-17, -36, -69, -193, -317, -419) were isolated on PSM media containing ammonium phosphate. The specific enzyme activities of A. ficuum mutants are 110 to 140% higher than that of wild type.
6-Phytase* ; Ammonium Compounds ; Aspergillus oryzae ; Aspergillus* ; Mass Screening* ; Mutagenesis* ; Oryza ; Phosphates ; Phytic Acid

No abstract available.
Hypothermia* ; Vena Cava, Inferior*

It is known that intraocular pressure becomes elevated over 65 mmHg when using a suction ring or fixation ring during LASIK. We intended to find out if transient elevation of IOP alters the microcirculation of the optic disc and retina. 10 patients were enrolled in this study. LASIK was performed on one eye using a suction ring and fixation ring. The other eye was used as a control. Heidelberg Retina Flowmetry[HRF] was performed preoperatively, postoperatively 1 day, 1 week, 3 weeks, and 3 months, respectively and analysis was done at the nasal and temporal neuroretinal rim, peripapillary retina, and optic cup. There was a statistically significant increase of flow at all sites on postoperative 1 day. Furthermore, increased flow was continued until postoperative 3 weeks at the temporal neuroretinal rim. However, the flow was normalized to the preoperative level by 3 months in all measured areas. No differences of flow were found when compared to the control eyes in each site.
Humans ; Intraocular Pressure ; Keratomileusis, Laser In Situ ; Microcirculation ; Optic Nerve* ; Retina* ; Suction

To cover soft tissue defect, various types of flaps have been used. Although lateral supramalleolar flap has been used as rotation flaps or reversed island flaps to cover defects around the foot and ankle, there has been no report about its application as a free flap. The lateral supramalleolar island flap has proves to be supplied constantly by the terminal branch of the peroneal arterys perforating branch. The free lateral supramalleolar flap has the same skin territory. Its vascular pedicle can be extended to the main peroneal artery and vena comitans, which can be obtained by dissecting the peroneal vessels between the distal tibia and fibula through the interosseous membrane. We have successfully transferred this free flap in eight patients from April 1994 to February 1995. All of the patients had full thickness skin defects which were caused by contact thermal burn, electrical burn, flame burn or traffic accident. There were no complications. From our experience, we feel that this new free flap have some advantages, induding vascular anatomy and long pedicle with large diameter and a relatively thin flap with minimal morbidity of donor site.
Accidents, Traffic ; Ankle ; Arteries ; Burns ; Fibula ; Foot ; Free Tissue Flaps* ; Humans ; Membranes ; Skin ; Surgical Flaps ; Tibia ; Tissue Donors

A major cause of less than ideal results following intraarticular anterior cruciate ligament (ACL) reconstruction has been imprecise nonanatomic tunnel position for graft placement either in the femur, the tibia, or both. Lack of defined constant reference landmarks for reproducible tunnel placement has contributed to this problem on both sides of the joint. The purpose of this study was to define constant anatomic intraarticular and extraarticular landmarks that can be used as definitive reference points to reproducibly create a tibial tunnel for ACL reconstruction that (1) results in an impingement-free graft in full extension; (2) positions the tibial tunnel such that the sagittal tunnel-plateau angle is parallel with the sagittal intercondylar roof-plateau angle in full extension to minimize shear seen by the graft at the tibial tunnel inlet. Preoperative full extension and 90degrees flexion lateral radiographs were obtained. Preoperative measurements of the tibial tunnel-tibial shaft angle and distance from inferior pole of patella to entry point of tibial tunnel were useful tool for impingement free, Blumensaats line paralleling ACL reconstruction with autogenous bone patella tendon bone graft. The average tibial tunnel-tibial shaft angle was 34+/-4.59degrees (male), 33.5+/-3.37degrees (female). The mean distance between patella inferior pole and tibial tunnel entry point was 6.62+/-0.61cm (male), 6.21+/-0.89cm (female). This study sought to define constant anatomic landmarks extraarticularly as well as intraarticularly that can be used to reliably create an ideal tibial tunnel for ACL reconstruction.
Anatomic Landmarks ; Anterior Cruciate Ligament ; Bays ; Femur ; Joints ; Patella ; Patellar Ligament ; Tibia ; Transplants

A clinical observation was made on 14 cases of transitional cell carcinoma of the renal pelvis and ureter whose diagnosis was confirmed pathologically at Kyung Hee University Hospital during the period tom November, 1971 to June, 1984. The results were as follows 1. The age of patient at the time of the diagnosis ranged from 42 to 75 years (mean 57.9 years), with highest incidence during the 6th decade. 11 tumors occurred in male patients and 3 in female patients, for a ratio of 3.7 : 1. 2. Common presenting symptoms were gross hematuria (92.8 %) and pain (28.5 %). 3. IVP was performed in all patients. A filling defect in the renal pelvis or ureter was found in 6 cases, and non-visualized kidney in 6 cases. RGP was performed in 9 patients and was useful to demonstrate a filling defect. 4. Urine cytology was positive in 6 of 13 patients. 5. Nephroureterectomy including a cuff of bladder was performed in 13 patients and simple nephrectomy was done in 1 patient who was misdiagnosed as hydronephrosis due to UPJ obstruction. 6. The tumors were staged into 4 Stages postoperatively. 5 patients had Stage A tumors, 2 Stage B, 5 Stage C and 2 Stage D. 1 patient had Grade I tumor, 6 Grade II, 4 Grade III and 3 Grade IV. In 3 patients, there were synchronous tumors in renal pelvis and ureter. Three patients subsequently developed bladder tumor. 7. 7 patients were died in 3 to 18 months (average 7.8 months) after initial treatment and all were categorized into Stage C and D.
Carcinoma, Transitional Cell ; Diagnosis ; Female ; Hematuria ; Humans ; Hydronephrosis ; Incidence ; Kidney ; Kidney Pelvis* ; Male ; Nephrectomy ; Ureter* ; Urinary Bladder ; Urinary Bladder Neoplasms