1.Characterization of monoclonal antibodies against human cytomegalovirus(HCMV) glycoprotein.
Eung Soo HWANG ; Ju Young SEOH ; Chung Gyu PARK ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1992;27(6):545-553
No abstract available.
Antibodies, Monoclonal*
;
Glycoproteins*
;
Humans*
2.Surgical treatment of the abdominal aortic aneurysm.
Suk Ha HWANG ; Eung Joong KIM ; Seung Pyung LIM ; Jang Soo HONG ; Young LEE
The Korean Journal of Thoracic and Cardiovascular Surgery 1993;26(5):355-359
No abstract available.
Aortic Aneurysm, Abdominal*
3.Establishment of Measurement of Human Cytomegalovirus with in situ ELISA.
Eung Soo HWANG ; Jin Hee KIM ; Chung Kyu PARK ; Chang Yong CHA
Journal of the Korean Society of Virology 2000;30(2):125-130
No Abstract Available.
Cytomegalovirus*
;
Enzyme-Linked Immunosorbent Assay*
;
Humans*
4.Von Recklinghausen' s Disease with Plexiform Neurofibroma , Giant Pigmentation , and Skeletal Abnormalities.
Sang Min HWANG ; Sung Ku AHN ; Beom Joo LEE ; Won Soo LEE ; Eung Ho CHOI
Korean Journal of Dermatology 1995;33(6):1179-1183
Plexiform neurofibroma is considered a pathognomic of Von Recklinghousen's disease, which involves the deep and large nerve trunk. These are large irregular nerve fascicles which result from an increase in endoneural matrix within individual nerve facicles, without an increased number of nerve fibers. We experenced a case of Von Recklinghausen's disease in a 24 year-old male who had variable cutaneous skeletal, and CNS lesions. He presented multiple neurofibromas, cafe-au-lait spots, and axillary freckles as common cutaneous lesions of NF-I and giant pigmentation, sacral hypertrichosis, and plexiform neurofibroma as unusual cutaneous lesions. Also he had a scoliosis, bowing deformity of the humerous and wedging deformity of the body of the 5th cervical spine as a skeletal manifestation and cortical calcification in the occipital area as a CNS manifestation.
Cafe-au-Lait Spots
;
Congenital Abnormalities
;
Humans
;
Hypertrichosis
;
Male
;
Melanosis
;
Nerve Fibers
;
Neurofibroma, Plexiform*
;
Neurofibromatoses
;
Neurofibromatosis 1
;
Pigmentation*
;
Scoliosis
;
Spine
;
Young Adult
5.Investigation of antigen related to the in vitro invasiveness of salmonella typhimurium through the Madin-Darby canine kidney(MDCK) epithelial cell monolayer.
Chung Gyu PARK ; Eung Soo HWANG ; Ju Young SEOH ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1991;26(6):553-562
No abstract available.
Epithelial Cells*
;
Salmonella typhimurium*
;
Salmonella*
6.A Clinical Study of Rosacea.
Tae Hyun KIM ; Sang Min HWANG ; Won Soo LEE ; Sung Ku AHN ; Eung Ho CHOI
Korean Journal of Dermatology 2000;38(5):583-588
No Abstract Available.
Rosacea*
7.Fetus in fetu of the retroperitoneal cavity.
Soo Young YOO ; Hwang Min KIM ; Soon Hee JUNG ; Eung Jo KIM
Journal of the Korean Surgical Society 1992;43(3):459-465
No abstract available.
Fetus*
8.A Case of Pruritic Folliculitis of Pregnancy.
Sang Min HWANG ; Eung Ho CHOI ; Won Soo LEE ; Sung Ku AHN
Korean Journal of Dermatology 1998;36(1):156-159
Pruritic folliculitis of pregnancy of pregnancy is a specific dermatoses of pregnancy which is characterized by erythematous follicular papules and pustules between the fourth and ninth month of pregnancy. It has usually resolved by 4 weeks of postpartum and has no adverse implications for mother and baby. We report a case of pruritic folliculitis of pregnancy in a 24-year-old primigravida woman who had had monomorphic erythematous follicular papules and pustules on her anterior chest wall and back. Histopathological findings of erythematous papules showed acute folliculitis and perifolliculitis. The skin lesions improved rapidly with 2 weeks of delivery without treatment.
Female
;
Folliculitis*
;
Humans
;
Mothers
;
Postpartum Period
;
Pregnancy*
;
Skin
;
Skin Diseases
;
Thoracic Wall
;
Young Adult
9.Immunoelectron Microscopic Analysis of Human Cytomegalovirus ( HCMV ) Antigens Reacting with Specific Monoclonal Antibodies.
Eung Soo HWANG ; Chang Yong CHA ; Hae Kyung PARK ; Ju Young SEOH
Journal of the Korean Society for Microbiology 1997;32(5):601-610
For the purpose of morphologic analysis of human cytomegalovirus (HCMV) antigens reacting with specific monoclonal antibodies, we observed HCMV particles after immunogold staining. HCMV was cultured in human fetal lung fibroblasts to be concentrated by polyethylene glycol 6,000. The HCMV stock was dropped onto Formva-coated grids and was fixed by 2% glutaraldehyde. The grids were reacted with MCMVA57, 93, 135 or with SCMVM1, 6, 14, 49 monoclonal antibodies (MoAbs) follwed by gold (10 nm)-conjugated goat anti-mouse IgG. Then the grids were stained with 2.5% uranyl acetate to be observed under Hitachi 500 or Jeol 1,200 electron microscope. When HCMV was reacted with SCMVM14 and SCMVM49 MoAbs, gold particles were adsorbed to virion envelopes, suggesting that the reactive antigens were envelope proteins. In cases of MCMVA135 and SCMVM6 MoAbs, gold particles were adsorbed to dense bodies as well as to virion envelope. These results, together with the previous results of immunologic and genetic characterization, suggested that the reactive antigens of MCMVA135 and SCMVM6 MoAbs were gB homologue and structural protein, respectively. In case of SCMVM1 MoAb, gold particles were adsorbed to capsids, envelopes, and dense bodies, suggesting that the reactive antigen was structural protein. In case of SCMVM8 MoAb, gold particles were observed between the envelopes and capsids, which space was supposed to be the tegument, suggesting that the reactive antigen was carbohydrate moiety of glycoprotein or its polymer. In cases of MCMVA57 and MCMVA93 MoAbs, gold particles were adsorbed to only dense bodies, suggesting that the reactive antigens were precursors of structural proteins.
Antibodies, Monoclonal*
;
Capsid
;
Cytomegalovirus*
;
Fibroblasts
;
Glutaral
;
Glycoproteins
;
Goats
;
Humans*
;
Immunoglobulin G
;
Lung
;
Polyethylene Glycols
;
Polymers
;
Virion
10.Analysis of env Subtypes of Porcine Endogenous Retrovirus in SNU Miniature Pigs.
Moa SA ; Chung Gyu PARK ; Eung Soo HWANG
Journal of Bacteriology and Virology 2014;44(1):75-83
All xenografts from pigs impose infection risk by porcine endogenous retrovirus (PERV). The purpose of this study was to investigate the env constructs with the comparison of the ratio of the competent form to the defective one of env in subtypes, PERV-A, PERV-B and PERV-C in different pig breeds. The results of PCR amplification of env represented that all env subtypes had more than two defective forms which cannot bind to host cells due to the absence of binding regions of env in miniature pigs, SNU and PWG, and farm pig breeds, Duroc, Yorkshire and Landrace. In addition, comparing the full sequences with the defective ones in three subtypes demonstrated that the present percentages of env sequences in defective PERV-A, PERV-B and PERV-C were approximately 50%, 38~45% and 4~11%, respectively, in SNU and PWG pigs whereas PERV-A and PERV-B occupied around 40 to 60% but PERV-C was not detected in farm pigs. Quantitative real-time PCR assays with primers and probes targeted to proline-rich region (PRR) of each env subtype were done to measure the copy numbers of each env subtype. When the reference was set with copy number of PERV-A, the ratio of those of PERV-B and PERV-C to the reference were 1.5 to 6.0 folds high in SNU and PWG pigs while 1.0 or less in farm pigs. These contradictory results of PERV-C constructs and copy numbers in SNU pigs suggests that many truncated or short defective sequences of PERV-C might be present in them.
Endogenous Retroviruses*
;
Heterografts
;
Polymerase Chain Reaction
;
Real-Time Polymerase Chain Reaction
;
Swine*
;
Transplantation, Heterologous