1.In vitro effects of monophosphoryl lipid A and Poly I:C combination on equine cells
Dong-Ha LEE ; Eun-bee LEE ; Jong-pil SEO ; Eun-Ju KO
Journal of Veterinary Science 2023;24(3):e37-
Background:
Toll-like receptor (TLR) agonists have been used as adjuvants to modulate immune responses in both animals and humans.
Objectives:
The objective of this study was to evaluate the combined effects of the TLR 4 agonist monophosphoryl lipid A (MPL) and the TLR 3 agonist polyinosinic:polycytidylic acid (Poly I:C) on equine peripheral blood mononuclear cells (PBMCs), monocyte-derived dendritic cells (MoDCs), and bone marrow-derived mesenchymal stromal cells (BM-MSCs).
Methods:
The PBMCs, MoDCs, and BM-MSCs collected from three mixed breed horses were treated with MPL, Poly I:C, and their combination. The mRNA expression of interferon gamma (IFN-γ), interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-12p40, tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1) was determined using real-time polymerase chain reaction.
Results:
The combination of MPL and Poly I:C significantly upregulated immunomodulatory responses in equine cells/ without cytotoxicity. The combination induced greater mRNA expression of pro-inflammatory cytokines IFN-γ and IL-6 than MPL or Poly I:C stimulation alone in PBMCs. In addition, the combination induced significantly higher mRNA expression of IL-1β, IL-6, and IL-12p40 in MoDCs, and IL-8, MCP-1, and VEGF in BM-MSCs compared to stimulation with a single TLR agonist.
Conclusions
The combination of MPL and Poly I:C can be used as a potential adjuvant candidate for vaccines to aid in preventing infectious diseases in horses.
2.A Case of Appendicitis with Liver Abscesses Developed during Allogeneic Hematopoietic Stem Cell Transplantation
Seul Bee LEE ; Jung Min LEE ; Sun Wha LEE ; Eun Sun YOO ; Kyung Ha RYU
Clinical Pediatric Hematology-Oncology 2014;21(2):172-176
A 10-year-old boy with severe aplastic anemia was admitted for allogeneic hematopoietic stem cell transplantation. After conditioning chemotherapy using cyclophosphamide, fludarabine, and antithymocyte immunoglobulin, he presented with fever and abdominal pain on day 0 of stem cell transplantation. After diagnosis of acute appendicitis with minor perforation, appendectomy was performed just after cell infusion. A week after the procedure, he showed two huge liver abscesses in S4 and S6 segments. We used broad spectrum antibiotics along with antifungal agents. Percutaneous drainage was attempted, but no fluid was removed and no microorganisms were isolated. After 7 weeks of antibiotics and antifungal therapy, liver abscesses showed improvement. We report a case of successfully treated appendicitis with liver abscesses in a severely neutropenic patient during allogeneic hematopoietic stem cell transplantation.
Abdominal Pain
;
Anemia, Aplastic
;
Anti-Bacterial Agents
;
Antifungal Agents
;
Appendectomy
;
Appendicitis
;
Child
;
Cyclophosphamide
;
Diagnosis
;
Drainage
;
Drug Therapy
;
Fever
;
Hematopoietic Stem Cell Transplantation
;
Humans
;
Immunoglobulins
;
Liver Abscess
;
Male
;
Stem Cell Transplantation
;
Typhlitis
3.Causes and Neonatal Outcome of Early-Onset Thrombocytopenia in Preterm Neonates.
Jung Min LEE ; Seul Bee LEE ; So Yeon SHIM ; Su Jin CHO ; Eun Ae PARK
Neonatal Medicine 2015;22(3):156-161
PURPOSE: Thrombocytopenia is the most common hematologic abnormality diagnosed in the neonatal intensive care unit (NICU). We investigated the causes and neonatal outcomes of early-onset thrombocytopenia in preterm neonates and compared clinical outcomes between neonates in an early-onset thrombocytopenia group who have received and those who have not received platelet transfusion in order to assess the clinical significance of early-onset thrombocytopenia in preterm infants. METHODS: We retrospectively reviewed the medical records of preterm neonates born earlier than 34 weeks' gestation who were admitted to the NICU between January 2005 and September 2014. 412 preterm neonates born earlier than 34 weeks' gestation were enrolled. The early-onset thrombocytopenia group (n=90) had a platelet count < or =150,000/mL within the first 72 hours of life. We investigated maternal and neonatal characteristics, and neonatal outcomes in the group and compared these with those of a control group (n=322). RESULTS: The neonates with early-onset thrombocytopenia tended to have lower gestational age, birth weight and Apgar scores than controls. Maternal hypertension and lower Apgar score at birth were predictive factors of early-onset thrombocytopenia. No differences in clinical outcomes were observed between the two groups. Clinical outcomes did not significantly different between the groups regardless of whether the neonates received platelet transfusion or not. CONCLUSION: Early-onset thrombocytopenia had no effect on the neonatal outcomes of the preterm infants. Platelet transfusion did not affect the clinical outcomes of the neonates in the early-onset thrombocytopenia group. Thus, we suggest a lager controlled study on early-onset thrombocytopenia in newborns in order to establish more-efficient treatment guidelines.
Apgar Score
;
Birth Weight
;
Gestational Age
;
Humans
;
Hypertension
;
Infant, Newborn*
;
Infant, Premature
;
Intensive Care, Neonatal
;
Medical Records
;
Parturition
;
Platelet Count
;
Platelet Transfusion
;
Pregnancy
;
Prognosis
;
Retrospective Studies
;
Thrombocytopenia*
4.In Vitro Culture of Nontransformed Cell Lines Derived from Rat Endometrial Epithelium and Stroma.
Byung Moon KANG ; Suk Won LEE ; Bee Dong CHAE ; Eun Hee KANG ; Hyung Sik CHU ; Chung Hoon KIM ; Yoon Seok CHANG ; Joo Hyun NAM
Korean Journal of Fertility and Sterility 1999;26(1):83-87
Since the blastocyst is broken and spreads out on a flat plastic culture dish (two dimensional culture) during in vitro development, it has been difficult to study the implantation process. It also has been difficult to analyse the interactions between endometrial epithelial and stromal cells because of the lack of a long-term in vitro model which can stimulate in vivo characteristics, as these cells eventually fail to proliferate or cease to express differentiated functions. Recently nontransformed cell lines, CUE-P and CUS-V2, derived from rat endometrial epithelium and stroma were reported. In this study, morphology of CUE-P and CUS-V2 was examined and oxytocin gene expression by CUE-P cells was demonstrated by RT-PCR. The CUE-P cells have a cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. In RT-PCR, same size of PCR products of oxytocin gene at hypothalamus, uterus and CUE-P cells were demonstrated. These results showed three dimensional culture system could be made by using the new cell lines.
Animals
;
Blastocyst
;
Cell Line*
;
Epithelium*
;
Fibroblasts
;
Gene Expression
;
Hypothalamus
;
Oxytocin
;
Plastics
;
Polymerase Chain Reaction
;
Rats*
;
Stromal Cells
;
Uterus
5.A Case of Langer-Giedion Syndrome with a De Novo Del(8)(q23q24.1).
Jung Min LEE ; Seul Bee LEE ; Sang Won LEE ; So Yeon SHIM ; Su Jin CHO ; Eun Ae PARK ; Jung Won HUH
Neonatal Medicine 2015;22(2):112-116
Lange-Giedion syndrome, or trichorhinophalangeal syndrome type 2 (TRPSII), is a clinical syndrome characterized by mild growth restriction, mental retardation, microcephaly and dysmorphic face. Bulbous nose, large protruding ears and loose redundant skin are distinguishing features, as well as lax joints and phalangeal abnormalities of the hands and multiple exostoses. TRPS1 and EXT1 gene deletion are responsible for this. Diagnosis is mainly based on clinical and radiographic features. In Korea, no cases of this disease have been reported thus far. Along with a review of the literature, we report a case of TRPSII in a neonate who had peculiar face representing TRPSII, polydactyly, Mullerian duct cyst, and ptosis and was found to have an interstitial deletion of 8q23-24.1.
Diagnosis
;
Ear
;
Exostoses, Multiple Hereditary
;
Gene Deletion
;
Hand
;
Humans
;
Infant, Newborn
;
Intellectual Disability
;
Joints
;
Korea
;
Langer-Giedion Syndrome*
;
Microcephaly
;
Nose
;
Polydactyly
;
Skin
6.Operation and Process Evaluation of a Community Meal Program for the Elderly in Rural Areas during Agricultural Off-Season Perceived by Cooking Volunteers
Jeong Sook BAE ; Sol Bee SEONG ; So Mang JANG ; Chang Hee YOO ; Young Suk LIM ; Young Mi LEE ; Hae Ryun PARK ; Kyung Eun LEE
Korean Journal of Community Nutrition 2019;24(4):277-289
OBJECTIVES: This study examined the practices of a community meal program for older adults in rural areas during the agricultural off-season. METHODS: A survey was conducted from December 12 to December 22, 2016. Self-administered questionnaires were distributed to 150 cooking volunteers, who had participated in the community meal program in 50 villages. A total of 114 responses were returned from 44 villages and used for data analysis. In addition, in-depth interviews were conducted with the volunteers of eight villages. RESULTS: Most of the cooking volunteers were 50 years old or older and they participated in serving older adults meals for good will. The cooking volunteers perceived that the older adults in their community did not eat various foods, had difficulties in grocery shopping, and frequently consumed salty foods. During the agricultural off-season, 40.9% of villages served the older adults meals 6–7 days a week and 95.5% provided meals for lunch. An average of 21 to 40 older adults were served meals in each village. The cooking volunteers reported that the food preparation and meal service times were sufficient, recipes provided were useful, and menus met the preference of the older adults. At the end of the program, they felt proud of serving meals for older adults in the community. An increased awareness of healthy eating, interest in health, and consumption of nutritious meals, a decrease in loneliness among older adults, and the promotion of fellowship in the community were rated highly. The cooking volunteers expected additional support for cooking personnel and insisted that the program should be provided for the entire agricultural off-seasons. CONCLUSIONS: The community meal program during the agricultural off-season for the elderly in rural areas was effective in improving the dietary life of older adults, relieving their feelings of isolation, and promoting fellowship of the community. The volunteers felt workload due to a shortage of volunteers but answered that they were rewarded by helping older adults in their community.
Adult
;
Aged
;
Cooking
;
Eating
;
Fellowships and Scholarships
;
Humans
;
Loneliness
;
Lunch
;
Meals
;
Reward
;
Statistics as Topic
;
Volunteers
7.Computed tomographic features of the temporomandibular joint in 10 Jeju horses
Seyoung LEE ; Eun-Bee LEE ; Kyung-Won PARK ; Hyohoon JEONG ; Kwang-Yun SHIN ; Young-Park KWEON ; Jong-pil SEO
Journal of Veterinary Science 2022;23(3):e44-
Background:
The equine temporomandibular joint (TMJ) has a complex anatomical structure that makes diagnosis of TMJ disorders difficult. Computed tomography (CT) is now available in equine medicine; hence, TMJ evaluation has become more convenient.
Objectives:
The objectives of this study were to describe the CT features of the TMJ in Jeju horses and to compare these features with those of Thoroughbreds.
Methods:
In this report, the TMJs of 10 Jeju horses (mean age: 4.5 ± 1.9 yr; mean body weight: 282.6 ± 40.3 kg) and 6 Thoroughbreds (mean age: 7.3 ± 1.6 yr; mean body weight: 479.7 ± 44.0 kg) were examined using CT. After CT scanning, the Hounsfield units (HU) and height to width ratio (H:W) of the mandibular condyle were measured.
Results:
The mean H:W in Jeju horses was significantly lower than that in Thoroughbreds.The mean HU in Jeju horses was lower than that in Thoroughbreds; however, the difference was not significant. The most frequent CT finding was an irregular medial margin of the mandibular condyle in both breeds.
Conclusions
In this study, the shape of the mandibular condyle in Jeju horses was flatter than that in Thoroughbreds. This report could be useful in evaluating the TMJ in Jeju horses. Moreover, CT could be a pragmatic choice for the examination of the TMJ in horses.
8.Small intestinal epiploic foramen entrapment in a seven-month pregnant Thoroughbred mare: a case report
Hyebin HWANG ; Seyoung LEE ; Kyung-won PARK ; Eun-bee LEE ; Taeyoung KANG ; Jong-pil SEO ; Hyohoon JEONG
Korean Journal of Veterinary Research 2023;63(2):e11-
A seven-month pregnant four-year-old Thoroughbred mare with colic weighing 600 kg was referred to the Jeju National University Equine Hospital. A physical examination and ultrasound suggested epiploic foramen entrapment. The patient underwent ileum resection and end-to-side jejunocecostomy. After surgery, the horse showed a consistent pain when introduced to feeding. A repeat laparotomy was performed to enlarge the stoma size with side-to-side jejunocecostomy. The repeat laparotomy was successful, and the mare gave birth uneventfully. This report describes a case of small intestinal epiploic foramen entrapment and the clinical outcomes of the 2 consecutive laparotomies a Thoroughbred mare in late gestation.
9.Endometrial cancer arising from atypical complex hyperplasia: The significance in an endometrial biopsy and a diagnostic challenge.
Jung Mi BYUN ; Dae Hoon JEONG ; Young Nam KIM ; En Bee CHO ; Ju Eun CHA ; Moon Su SUNG ; Kyung Bok LEE ; Ki Tae KIM
Obstetrics & Gynecology Science 2015;58(6):468-474
OBJECTIVE: We investigated the features of endometrial hyperplasia with concurrent endometrial cancer that had been diagnosed by endometrial sampling. Further, we attempted to identify an accurate differential diagnostic method. METHODS: We retrospectively studied 125 patients who underwent a diagnostic endometrial biopsy or were diagnosed after the surgical treatment of other gynecological lesions, such as leiomyoma or polyps. Patients were diagnosed between January 2005 and December 2013 at Busan Paik Hospital. Clinical and histopathological characteristics were compared in patients who had atypical endometrial hyperplasia with and without concurrent endometrial cancer. RESULTS: The patients were grouped based on the final pathology reports. One hundred seventeen patients were diagnosed with endometrial hyperplasia and eight patients were diagnosed with endometrioid adenocarcinoma arising from atypical hyperplasia. Of the 26 patients who had been diagnosed with atypical endometrial hyperplasia by office-based endometrial biopsy, eight (30.8%) were subsequently diagnosed with endometrial cancer after they had undergone hysterectomy. The patients with endometrial cancer arising from endometrial hyperplasia were younger (39.1 vs. 47.2 years, P=0.0104) and more obese (body mass index 26.1+/-9.6 vs. 23.8+/-2.8 kg/m2, P=0.3560) than the patients with endometrial hyperplasia. The correlation rate between the pathology of the endometrial samples and the final diagnosis of endometrial hyperplasia was 67.3%. CONCLUSION: In patients with atypical endometrial hyperplasia, the detection of endometrial cancer before hysterectomy can decrease the risk of suboptimal treatment. The accuracy of endometrial sampling for the diagnosis of concurrent endometrial carcinoma was much lower than that for atypical endometrial hyperplasia. Therefore, concurrent endometrial carcinoma should be suspected and surgical intervention should be considered in young or obese patients who present with atypical endometrial hyperplasia.
Biopsy*
;
Busan
;
Carcinoma, Endometrioid
;
Diagnosis
;
Endometrial Hyperplasia
;
Endometrial Neoplasms*
;
Female
;
Humans
;
Hyperplasia*
;
Hysterectomy
;
Leiomyoma
;
Pathology
;
Polyps
;
Retrospective Studies
10.In vitro fertilization outcome in women with diminished ovarian reserve.
Bo Hyon YUN ; Gieun KIM ; Seon Hee PARK ; Eun Bee NOE ; Seok Kyo SEO ; SiHyun CHO ; Young Sik CHOI ; Byung Seok LEE
Obstetrics & Gynecology Science 2017;60(1):46-52
OBJECTIVE: This study aimed to identify factors that affect in vitro fertilization (IVF) outcomes in women with diminished ovarian reserve (DOR). METHODS: We reviewed 99 IVF cycles in 52 women with DOR between September 2010 and January 2015. DOR was defined as serum anti-Müllerian hormone level of <1.1 ng/dL or serum follicle-stimulating hormone level of ≥20 mIU/mL. Total 96 cycles in 50 patients were evaluated after excluding fertility preservation cases. RESULTS: The clinical pregnancy rate was 11.5% per cycle, and the total cancellation rate was 34.4%. Clinical pregnancy rate was significantly associated with the antral follicle count and the cause of the DOR. Age, serum anti-Müllerian hormone and follicle-stimulating hormone levels, antral follicle count, peak estradiol level, and the cause of DOR were significantly associated with cycle cancellation. However, history of previous ovarian surgery remained as a significant factor of clinical pregnancy (model 1: odds ratio [OR] 10.17, 95% confidence interval [CI] 1.46 to 70.84, P=0.019; model 2: OR 10.85, 95% CI 1.05 to 111.71, P=0.045). In cancellation models, idiopathic or previous chemotherapy group showed borderline significance (model 1: OR 3.76, 95% CI 0.83 to 17.04, P=0.086; model 2: OR 3.15, 95% CI 0.84 to 11.84, P=0.09). CONCLUSION: DOR caused by previous ovarian surgery may show better pregnancy outcome, whereas that caused by chemotherapy could significantly increase the cycle cancellation rate. Furthermore, patients with DOR who previously received gonadotoxic agents may show reduced efficacy and increased risk of IVF cycle cancellation.
Drug Therapy
;
Estradiol
;
Female
;
Fertility Preservation
;
Fertilization in Vitro*
;
Follicle Stimulating Hormone
;
Humans
;
In Vitro Techniques*
;
Odds Ratio
;
Ovarian Reserve*
;
Pregnancy
;
Pregnancy Outcome
;
Pregnancy Rate