Myxopapillary ependymoma generally arise in the conus medullaris and filum terminale of adult spinal cord. These tumors are readily recognized due to unique histopathologic features, however, their cytologic features are not well described. When only a tiny sample is obtained, cytologic examination using crush preparation may be a useful diagnostic tool to help appropriate intraoperative diagnosis. We present the crush cytologic features of myxopapillary ependymoma arising in thoracic and lumbar spinal cord of a 13-year-old boy. The patient had complained of paraparesis and back pain for 1 month. The MRI image revealed a relatively well demarcated intramedullary mass in T11-L1 levels. Crush preparation for cytology were performed by biopsy material. Crush cytologic findings revealed high cellularity and small sized branching papillary clusters on fibrillary or mucinous background. The tumor cells had uniform round or elongated nuclei. The cytoplasmic process of tumor cells were attached to the vascular wall. Between the tumor cells and vascular walls, the perivascular collar of globoid acellular stroma with metachromatic reaction on toluidin blue stain was noted. The crush preparation of myxopapillary ependymoma is considered as a simple and highly accurate diagnostic tool for differentiation from other intramedullary neoplasms of central nervous system.
Adult ; Male ; Female ; Infant, Newborn ; Humans ; Biopsy

No Abstract Available.
Methicillin Resistance* ; Methicillin* ; Methicillin-Resistant Staphylococcus aureus* ; Staphylococcus aureus* ; Staphylococcus*

No Abstract Available.
Vibrio parahaemolyticus* ; Vibrio* ; Virulence Factors* ; Virulence*

No Abstract Available.
Methicillin Resistance* ; Methicillin* ; Methicillin-Resistant Staphylococcus aureus* ; Staphylococcus aureus* ; Staphylococcus*

No Abstract Available.
Vibrio parahaemolyticus* ; Vibrio* ; Virulence Factors* ; Virulence*

Sixteen strains of LT-producing enterotoxigenic E. coli 0128 which were isolated from diarrheal patient's stool in Pusan University Hospital, were serotyped and analyzed for plasmid DNA profile, MRHA of human blood cells, and also tested for possession of LT, ST, aggA, EAST1 genes by the PCR method and analyzed the RAPD pattern. Screening sensitivity for ETEC by salting out test was 87.5%. These data suggest that hydrophobicity test using salting out is rapid, inexpensive, and simple screening test for ETEC. CFAs were identified in 87.5% of strains; 43.75% the strains harbored CFA/I, 43.75% CFA/II, and 12.5% expressed none of these CFAs. For plasmid profiles, 12 strains had 60 MDa plasmid and several smaller plasmids. The strains showed 5 types of plasmid profiles. By PCR, LT gene but not ST gene was detected from all of the 16 strains EAST1 gene was detected from 14 strains. Ten strains could be differentiated to 3 patterns by chromosomal DNA fingerprint. The chromosomal DNA fingerprinting is considered very useful for the epidemiological study.
Blood Cells ; Busan ; DNA ; DNA Fingerprinting ; Enterotoxigenic Escherichia coli* ; Humans ; Hydrophobic and Hydrophilic Interactions ; Mass Screening ; Plasmids ; Polymerase Chain Reaction ; Virulence Factors* ; Virulence*

No abstract available.
Diuresis* ; Humans ; Infant, Newborn*

No abstract available.
Knee Joint* ; Knee*

No abstract available.
Humans

PURPOSE: Hemolytic uremic syndrome(HUS) is the most common cause of renal failure in childhood in western countries, but it has been a relatively rare disease in Korea. To analyze the clinical and epidemiologic characteristics of HUS in Korea, we reporte our experience, METHODS: We reviewed retrospectively the medical records of 9 patients who were diagnosed the hemolytic uremic syndrorne at Pusan National University during the period from January 1984 to December 1998. RESULTS: The age at presentation ranged from 3 years to 12 years old(mean: 5.9 years). Six of the children were typical and three patients were atypical. Six cases had grossly bloody stools and seven had abdominal pain. Oliguria developed in four cases and gross hematuria in eight cases. One case had seizure and letargy. Verotoxin producing E. coli(VTEC) were found in the stool specimens of two pa- tients. One was the serotype 0157:H7 and the other was the serotype 026:H-, Olll:H21. Verotoxin 1 (VT1) genes were identified by PCR in both of the cases. All patients required conservative treatment. Two patients were treated with peritoneal dialysis, one patient with hemodialysis and one patient with plasmapheresis. Of 9 case, 6 recovered without residual sequelae, but two patients died, and one patient progressed to end stage renal failure. CONCLUSION: Two thirds of our cases were typical type of HUS, which is now the predominant type among Korean childhood HUS sufferers. From this study, we suggest that patients diagnosed with typical HUS should be studied for the verotoxin of E. coli isolated from stool.
Abdominal Pain ; Busan ; Child* ; Hematuria ; Hemolytic-Uremic Syndrome* ; Humans ; Korea ; Medical Records ; Oliguria ; Peritoneal Dialysis ; Plasmapheresis ; Polymerase Chain Reaction ; Rare Diseases ; Renal Dialysis ; Renal Insufficiency ; Retrospective Studies ; Seizures ; Shiga Toxins