No abstract available.
Animals ; Capsaicin* ; Ganglia, Spinal* ; Rats* ; Spinal Nerve Roots*

Recently, it has been postulated that diabetic autonomic neuropathy is caused by reduction in availability of nerve growth factor (NGF) in enteric nervous system. This experiments were performed to determine the changes of the distribution of enteric neuropeptide by diabetes and these changes could be prevented by administration of NGF. Sprague Dawley rats (200~250gm) were made diabetic by a single intraperitoneal injection of streptozotocin 65 mg/kg in saline. Recombinant human NGF (Sigma, Co., Ltd.) were administered at a dose of 500ng/kg subcutaneously every day for consecutive 4 weeks after streptozotocin administration. After 4 weeks, rats were anesthetized with ether and perfused with 4% paraformaldehyde. ileum was dissected and prepared by whole mount preparation method. Prepared segments were immunostained for substance p, calcitonin gene-related peptide, vasoactive intestinal peptide, and galanin by PAP technique. For the observation of the interstitial cells of Cajal, segments were immersed in Champy-Maillet solution for 2 days Results obtained were as follows: 1. In myenteric plexus of diabetic rats, substance P-like and VIP-like immunoreactivity were not changed compared with that of the control group. CGRP-like and galanin-like immunoreactivity were decreased in diabetic group and immunoreactive cells for CGRP and galanin were also decreased 18.1% (P<0.01) and 43.7% (P<0.01) respectively. 2. In NGF administerd diabetic group, immunoreactivity of substance p, VIP, galanin in myenteric plexus were slightly increased and immunoreactive cells for substancre p, VIP, galanin were almost the same as that of the control group. However, immunoreactive cells for CGRP of myenteric plexus were not changed by NGF. 3. In submucous plexus of diabetic rats, immunoreactivity of all four neuropeptides(substance p, CGRP, VIP, galanin) were decreased compared with that of the control group. Immunoreactive cells for substance p, CGRP, VIP, and galanin were also decreased in 38.8%, 77.6%, 33.0%, and 35.7%, respectively (P<0.01). 4. In NGF administered diabetic group, immunoreactivities of substance p, VIP and galanin in submucous plexus were increased and the immunoreactive cells were increased significantly compared to diabetic group. However, immunoreactive cells for CGRP of submucous plexus were not changed by NGF. 5. Interstitial cells of Cajal of diabetic group were decreased 7.4% ovoidal cells (A type) and 28.3% round cells (B type) In NGF administered group, the morphology and the number of ICC were not different to the control group. With the above results, it could be assumed that NGF prevent the damage of neurotransmitter and ICC in enteric nervous system.
Animals ; Calcitonin Gene-Related Peptide ; Diabetic Neuropathies ; Enteric Nervous System ; Ether ; Galanin ; Humans ; Ileum ; Injections, Intraperitoneal ; Interstitial Cells of Cajal ; Myenteric Plexus ; Nerve Growth Factor* ; Neuropeptides ; Neurotransmitter Agents ; Peristalsis* ; Rats* ; Rats, Sprague-Dawley ; Streptozocin ; Submucous Plexus ; Substance P ; Vasoactive Intestinal Peptide

The expression of c-fos and c-jun in the brain of the rat after capsaicin treatment was investigated by in situ hybridization, dot blot hybridization and immunocytochemical methods. Adult male Sprague-Dawley rats[200g] were used for this study. The first set of rats received a single subcutaneous injection of capsaicin[50mg/Kg] dissolved in 10% Tween-80 and 10% ethanol in saline. The rats were decapitated 1, 3, 5, 10, 24, 72 hours and 1 week after capsaicin treatment. The control set of rats were treated with saline instead of capsaicin. In situ hybridization and dot blot hybridization were carried out. O1igonucleotide probe complimentary to c-fos mRNA sequences were used for this study and labeling of oligonucleotides was accomplished using the DNA tailing kit. The expression of c-fos mRNA on the nucleus of neurons in in situ hybridization was observed throughout the brain, and was especially abundant in the olfactory cortex, nucleus of diagonal band of Broca, habenular nuclei, periaqueductal gray, parabrachial nucleus, entopeduncular nucleus, ventral posterolateral nucleus of the thalamus and cerebellum. Compared to the control rats, c-fos mRNA were increased 24 hours after capsaicin injection and gradually decreased after 72 hours, returning to the normal control level 1 week after capsaicin injection. c-fos mRNA was detected only 1 week after capsaicin injection in the various areas of the brain. The fos protein-like immunoreactivity was initially somewhat decreased at 24 hours, but increased at 72 hours and reactions was maximally observed at 1 week after capsaicin treatment. But Jun protein immunoreactivity was not increased, on the contrary, it was even decreased both in numbers of reactive cells and immunoreactivity 1 week after capsaicin injection. From the above results, c-fos gene expression was pronounced in the nucleus concerned with pain, olfaction and taste such as VPL nucleus of the thalamus, olfactory cortex and parabrachial nucleus, in the limbic system concerned with stress and emotion such as nucleus of diagonal band of Broca, periaqueductal gray and habenular nucleus, in the structure concerned with somatic motor function such as entopeduncular nucleus and cerebellum. Also, the c-fos gene was activated by the capsaicin early in the course of effects, then the fos protein increased as a results of c-fos activation. On the other hand, c-jun did not respond to capsaicin treatment early in the course, but Jun protein decreased late in the course of capsaicin effects.
Adult ; Animals ; Brain* ; Capsaicin* ; Cerebellum ; DNA ; Entopeduncular Nucleus ; Ethanol ; Genes, fos ; Habenula ; Hand ; Humans ; In Situ Hybridization ; Injections, Subcutaneous ; Limbic System ; Male ; Neurons ; Olfactory Pathways ; Oligonucleotides ; Periaqueductal Gray ; Rats* ; Rats, Sprague-Dawley ; RNA, Messenger ; Septal Nuclei ; Smell ; Thalamus ; Ventral Thalamic Nuclei

PURPOSE: The aim of this study was to determine the role of renal ultrasonography in neonates with isolated preauricular tags. METHODS: We performed a retrospective study of 10,997 newborn infants delivered from January 1995 to June 2003 in Wonkwang University Hospital. Nineteen newborns born with isolated preauricular tags were assessed for renal anomalies by performing renal ultrasonography within one week after birth. The study group was compared with a control group of 25 healthy neonates without preauricular tags during the same period, also using renal ultrasonography. RESULTS: Preauricular tags were detected in 19 of 10,997(0.17%) neonates; 57.9% were found on the right side. Renal anomalies in neonates with isolated preauricular tags were detected in four infants (21.1%); this incidence was higher, but was not significant, compared with the control group. Types of anomalies were mild hydronephrosis of grade 1(3 cases) and 2(1 case) which were normalized within one year. CONCLUSION: Routine renal ultrasonography is not recommended for newborn infant with isolated preauricular tags.
Humans ; Hydronephrosis ; Incidence ; Infant ; Infant, Newborn* ; Parturition ; Retrospective Studies ; Ultrasonography

No abstract available.
Sturge-Weber Syndrome*

No abstract available.
Gallbladder* ; Polyps*

Acute pancreatitis is an inflammatory disease of the pancreas. Acute abdominal pain is the most common symptom, and increased concentrations of serum amylase and lipase confirm the diagnosis. Pancreatic injury is mild in 80% of patients, who recover without complications. The remaining patients have a severe disease with local and systemic complications. Acute pancreatitis is a hypercatabolic state resulting in rapid loss of body weight, fat and protein. Nutritional support is an integral part of patient care and is started early in the course of disease. Patients with mild to moderate disease (80% of patients) do not require enteral nutrition (EN) or parenteral nutrition (PN), as they will begin oral feeding within 4 days of presentation. Nutritional support is needed for severe disease, EN is preferred over PN, and use PN when EN is contraindicated or not feasible. Most groups have used nasojejunal feeding, which has difficulties in maintenance of the tube position and patency. Nasogastric feeding in severe acute pancreatitis has shown little difference in terms of clinical outcome from nasojejunal feeding. In this review, we review the role, methods, and clinical implications of nutritional supports in acute pancreatitis and also present recently recommended standard guidelines.
Abdominal Pain ; Amylases ; Body Weight ; Enteral Nutrition ; Humans ; Lipase ; Nutritional Support ; Pancreas ; Pancreatitis ; Parenteral Nutrition ; Patient Care

Acute pancreatitis is an inflammatory disease of the pancreas. Acute abdominal pain is the most common symptom, and increased concentrations of serum amylase and lipase confirm the diagnosis. Pancreatic injury is mild in 80% of patients, who recover without complications. The remaining patients have a severe disease with local and systemic complications. Acute pancreatitis is a hypercatabolic state resulting in rapid loss of body weight, fat and protein. Nutritional support is an integral part of patient care and is started early in the course of disease. Patients with mild to moderate disease (80% of patients) do not require enteral nutrition (EN) or parenteral nutrition(PN), as they will begin oral feeding within 4 days of presentation. Nutritional support is needed for severe disease, EN is preferred over PN, and use PN when EN is contraindicated or not feasible. Most groups have used nasojejunal feeding, which has difficulties in maintenance of the tube position and patency. Nasogastric feeding in severe AP has shown little difference in terms of clinical outcome from nasojejunal feeding. In this review, we review the role, methods, and clinical implications of nutritional supports in acute pancreatitis and also present recently recommended standard guidelines.
Abdominal Pain ; Amylases ; Body Weight ; Diagnosis ; Diet* ; Enteral Nutrition ; Humans ; Lipase ; Nutritional Support ; Pancreas ; Pancreatitis* ; Parenteral Nutrition ; Patient Care

The statistical study of new outpatients of skin problems seen in dermatologic department of Seoul Red Cross Hospital from l976 to 1980 was done and the 10 most common dermatoses were analysed in detail, The results were summarized as follows: 1) Of 19,679 outpatients, males were 10,530(53.5%) and females were 9,149(46.5%). The sex ratio of male to female was 1. 15: l. 2) The most common dermatoses were atopic dermatitis/eczema(16. 9%), fungal infection(16. 2%), contact dermatitis(15. 3%), scabies(10. 7%), urticaria(10. 6%), acne(9. 5%), pyoderma(8. 3%), insect bite(5. 6%), syphilis(4. 1%) and psoriasia (2. 8%). 3) Of 10 most common dermatoses, contact dermatitis, acne, scabies and urticaria were revealed to have a tendency to increase year by year, syphilis and psoriasis were constant pattern and other common dermatoses showed irregular pattern in their incidence. 4) The sex ratio of male to female of l0 most common dermatoses was l. 23: l. Fungal infection, scabies, pyoderma, insect bite, syphilis and psoriasis wereoutnumbered in male and atopic dermatitis/eczema, contact dermatitis, urticaria and acne were outnumbered in female. 5) Atopic dermatitis/eczema & scabies were prevalent in winter, acne, fungal infection, urticaria and pyoderma in summer, and contact dermatitis in spring and summer. 6) The most frequent age groups of atopic dermatitis/eczema were 1 to 10 years, acne and fungal infection, 11 to 30 years, and contact dermatitis and fungal infection, 31 to 50 years.
Acne Vulgaris ; Dermatitis, Contact ; Female ; Humans ; Incidence ; Insect Bites and Stings ; Insects ; Male ; Outpatients* ; Psoriasis ; Pyoderma ; Red Cross ; Scabies ; Seoul ; Sex Ratio ; Skin ; Skin Diseases ; Statistics as Topic* ; Syphilis ; Urticaria

A 32-year-old male patient with uncontrolled diabetes mellitus was affected with hyperlipoproteinemia type II His skin lesion showed yellowish papulsr tuberoruptive xanthoma on forearm, shoulder, elbow and knee. Besides skin eruption, he showed abnormal liver function test and right bundle branch block on EKG. The laboratory examinations revealed increase of serum cholesterol, triglycerides. and fasting blood sugar, and slight turbid color of fasting blood serum which had been kept standing at 4C for 24 hrs. On the agarose electrophoresis, bands of different densities of LDL and VLDL in beta and pre-beta position Were noted. Authors discussed here about laboratory characteristics, clinical manifestations and differential diagnosis of hyperlipoproteinemia type II and III.
Adult ; Blood Glucose ; Bundle-Branch Block ; Cholesterol ; Diabetes Mellitus* ; Diagnosis, Differential ; Elbow ; Electrocardiography ; Electrophoresis ; Fasting ; Forearm ; Humans ; Hyperlipoproteinemia Type II* ; Hyperlipoproteinemias* ; Knee ; Liver Function Tests ; Male ; Sepharose ; Serum ; Shoulder ; Skin ; Triglycerides ; Xanthomatosis