1.Myoblast Transfer Therapy on mdxMouse.
Woo Nam MOON ; Chang Sub UHM ; Eun Kyung PARK ; Jae Yong AHN
The Journal of the Korean Orthopaedic Association 2002;37(2):281-287
PURPOSE: To observe dystrophin formation and histological improvement in dystrophic muscle of mdx mouse after normal myoblast injection. MATERIALS AND METHODS: Cultured myoblasts from genetically normal rats were injected into the right quadriceps femoris of a 6-week-old mdx mouse (n=9). dPBS was injected into the left quadriceps femoris as a control. One, 2, and 3 months after injection, The control and experimental group were compared histologically and by dystrophin immunostaining. RESULTS: When compared with controls 3 months postoperatively, quadriceps femoris in the experimental group exhibited greater cross-sectional area and total fiber number, and the experimental animals contained more normal-appearing and less abnormalappearing fibers than the control group. Most of the fibers in the experimental group showed positive results in dystrophin immunostaining, whereas immunostaining of mdx muscle fibers in the control group was completely negative. CONCLUSION: This study shows that normal myoblast injection improved the muscle architecture histologically and produced dystrophin protein in dystrophic muscle.
Animals
;
Dystrophin
;
Mice
;
Mice, Inbred mdx
;
Muscular Dystrophies
;
Myoblasts*
;
Quadriceps Muscle
;
Rats
2.Intramedullary Spinal Lesions Involving the Conus Medullaris: MR Imaging Features for Differential Diagnosis.
Na Lae EUN ; Sung Jun AHN ; Tae Sub CHUNG ; Yong Eun CHO ; Keun Su KIM ; Sung Uk KUH ; Sang Hyun SUH
Journal of the Korean Society of Magnetic Resonance in Medicine 2014;18(2):144-150
PURPOSE: Intramedullary spinal lesions in the conus medullaris (CM), including tumors and vascular lesion, are rarely reported. We reported various MR features of intramedullary spinal cord lesions involving the CM including ependymoma, hemangioblastomas, dermoid cyst, ventriculus terminalis and spinal AVF and tried to discuss them for differential diagnosis. MATERIALS AND METHODS: Six patients (male: female = 4:2, mean age = 44.3 year old) were enrolled from the clinical database of our institute from 2004 to 2010 and their radiological images and clinical symptoms were reviewed retrospectively. All patients had taken initial and postoperative MRI with contrast enhancement using gadopentate dimeglumine (Gd-DTPA). These images were analyzed by tumor size, location, signal intensity relative to the spinal cord, vascular flow voids, syrinx or cyst, edema and enhancement pattern. RESULTS: Contrast enhancement was seen in all intramedullary masses. An eccentric enhancing nodule was noted in two hemangioblastomas and unusual peripheral rim enhancement with septation was seen in ventriculus terminalis. Patchy enhancement of the CM was observed in spinal arteriovenous fistula (AVF). Extensive cord edema adjacent to the intramedullary lesions was seen in four cases and syrinx was noted in three cases. Vascular signal voids were found in two hemangioblastomas and one spinal AVF. CONCLUSION: In evaluation of intramedullary spinal lesions in the CM, it is necessary to consider these unusual MR findings and discriminate various pathologies with prudence and caution.
Arteriovenous Fistula
;
Conus Snail*
;
Dermoid Cyst
;
Diagnosis, Differential*
;
Edema
;
Ependymoma
;
Female
;
Hemangioblastoma
;
Humans
;
Magnetic Resonance Imaging*
;
Pathology
;
Retrospective Studies
;
Spinal Cord
3.Interleukin-1beta Modulates Proliferation, Interleukin-6 and Interleukin Receptor Expression in PC-3 and DU-145 Prostatic Cancer Cells.
Soon Chul MYUNG ; Seung Young AHN ; Seung Young OH ; Eun Ha WON ; Eun Sub PARK ; Kyung Do KIM
Korean Journal of Urology 2004;45(8):810-816
Purpose: IL-1 is a multifunctional proinflammatory cytokine. As the proliferative effects of IL-6 and IL-6 receptor expressions on prostatic cancer cells in response to IL-1 have not been determined, the effects of IL-1 on prostatic cancer cell lines were investigated. Materials and Methods: PC-3 and DU-145 cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum. Cell cultures were supplemented with various concentrations of IL-1 (0, 1, 10, 20 and 40ng/ml), and the MMT growth assay performed. PC-3 and DU-145 cells were treated for 2, 4, 8, 12 and 24 h both with and without IL-1. IL-6 and IL-6 receptor (IL-6R) mRNA expressions were investigated using RT-PCR, and the IL-6 levels in cultured supernatant measured by ELISA. Results: The viability of both PC-3 and DU-145 cells decreased after IL-1 treatment (10, 20 and 40ng/mul). With 40ng/ml the IL-1, IL-6 and IL-6RmRNA expressions were lower in PC-3 cells, but unchanged in DU-145 cells, whereas the IL-6 protein production was higher in both PC-3 and DU-145 cells. Conclusions: IL-1 inhibited the proliferation of both PC-3 and DU145 cells. In the PC-3 cells, IL-1 decreased the expressions of IL-6 and IL-6R mRNA, but paradoxically increased the IL-6 production. In the DU-145 cells, IL-1 treatment did not affect the IL-6 or IL-6R mRNA expressions, but the IL-6 production was increased. This discrepancy between IL-1-induced IL-6 mRNA and protein production may be mediated by modification to the protein synthesis or an increased cellular excretion.
Cell Culture Techniques
;
Cell Line
;
Enzyme-Linked Immunosorbent Assay
;
Interleukin-1
;
Interleukin-1beta*
;
Interleukin-6*
;
Interleukins*
;
Prostatic Neoplasms*
;
Receptors, Interleukin*
;
Receptors, Interleukin-6
;
RNA, Messenger
4.Primary Amyloidosis Involving Mediastinal and Hilar Lymph Nodes: A Case Report.
Myeong Im AHN ; Jeong Su JEON ; Jee Young KIM ; Eun Suk CHA ; Kyung Sub SHINN ; Chi Hong KIM ; Eun Deok CHANG
Journal of the Korean Radiological Society 1996;34(5):617-619
Amyloidosis is a rare systemic disease caused by extracellular accumulation of insolubel fibrillar material invarious tissues. The radiographic findings of amyloidosis involving the lymph nodes have not been previously reported in Korea, and we report a rare case of primary amyloidosis involving the mediastinal and hilar lymphnodes, with CT and radiographic appearances.
Amyloidosis*
;
Korea
;
Lymph Nodes*
5.The Effect of Continuing Breastfeeding during Treatment of Breast Milk Jaundice.
Eun Sub AHN ; Minkyun KIM ; Yeon Kyung LEE ; Sun Young KO ; So Young YOON ; Goun JUNG ; Sung Won PACK ; Son Moon SHIN
Korean Journal of Perinatology 2013;24(4):259-264
PURPOSE: Though it is a general and common method to temporarily stop breast feeding and use whole milk instead for neonatal breast milk jaundice, it may cause some difficulties in continuing breast feeding after the recovery. We study the effect of continuing breast feeding on the treatment of breast milk jaundice and the success of breast feeding afterwards. METHODS: We retrospectively analyzed the medical records of 59 neonates who were admitted to Cheil general hospital from Jan 2008 to Aug 2012 for phototherapy due to breast milk jaundice. Subjects were divided into two groups, one with continuing breast feeding (35 cases) during treatment and the other with stopping breast feeding (24 cases). We examined and compared the changes in the level of serum total bilirubin between two groups, as well as the difficulties the mothers might had in continuing or restarting breast feeding after the discharge. RESULTS: There was no significant difference in times of treatment (until reaching the level of serum total bilirubin <13 mg/dL) between two groups (P=0.066). However, the group with temporary stop of breast feeding had difficulties such as nipple confusion and breast engorgement compared to breast feeding group (P=0.001). In long-term follow up, the breast feeding duration (P=0.017) and the rate of exclusive breast feeding for 6 months (P=0.024) were also significantly higher in breast feeding group. CONCLUSIONS: We suggest that continuing breast feeding while treating breast milk jaundice is helpful both for successfully continuing breast feeding and preventing problems after discontinuing breast feeding.
Bilirubin
;
Breast Feeding*
;
Breast*
;
Follow-Up Studies
;
Hospitals, General
;
Humans
;
Infant, Newborn
;
Jaundice*
;
Medical Records
;
Methods
;
Milk
;
Milk, Human*
;
Mothers
;
Nipples
;
Phototherapy
;
Retrospective Studies
6.Significance of Heat Shock Protein 70 and Sulphomucin Expression in Gastric Adenocarcinomas.
Eun A CHOI ; Ki Hun JUNG ; Min Gu OH ; Byung Ook CHUNG ; Joon Hee LEE ; Sung Han BAE ; Woo Sub AHN ; Joung Wook SUH ; Chang Yung JUNG ; Dong Hoon KIM
Journal of the Korean Surgical Society 1999;57(1):47-56
BACKGROUND: The heat shock proteins (HSPs) are stress-responsive genes present in all species and play a major role in many cellular processes. These proteins are highly conserved molecules whose expression is induced in eukaryotic cells by a variety of environmental stresses. These proteins can also be expressed in virally transformed cells and cancer cells. Especially, HSP70 is found at a higher level in growing cells than in resting cells. Sulphomucin is secreted by immature foveolar cells of stomach and expressed in gastric adenocarcinomas. Also, it is known that the population of sulphomucin-producing cells increases with long-lasting stress. The purpose of this study was to determine HSP70 and sulphomucin expressions in gastric adenocarcinoma and the significance of expressions. METHODS: Thirty-one paraffin-embeded surgical specimens of gastric adenocarcinomas were obtained from April 1992 to March 1995 and were selected for analysis. The expressions of HSP70 and sulphomucin were analyzed by immunohistochemical staining with HSP70 monoclonal antibody and the Spicer (HID) method. RESULTS: The expressions of HSP70 and sulphomucin were positive in 13 (42%) cases and 11 (35%) cases, respectively. The expression of HSP70 correlated with neither clinopathological factors nor sulphomucin expression. There was a significant correlation not only between sulphomucin expression and histologic differentiation (p=0.001) but also between disease-free survival and sulphomucin expression. CONCLUSIONS: Sulphomucin expression in gastric adenocarcinoma may be useful as a prognostic factor of gastric adenocarcinomas.
Adenocarcinoma*
;
Disease-Free Survival
;
Eukaryotic Cells
;
Heat-Shock Proteins*
;
Hot Temperature*
;
HSP70 Heat-Shock Proteins*
;
Stomach
7.Effect of Parathyroid Hormone on Left Ventricular Hypertrophy in Aged Ovariectomized Rats.
Chang Gyu PARK ; Dong Joo OH ; Hong Seog SEO ; Chang Sub YHM ; Han Geam KIM ; Eung Joo KIM ; Eun Mi LEE ; Jung Chun AHN ; Woo Heuk SONG ; Do Sun YIM ; Young Hoon KIM ; Wan Joo SHIM ; Young Moo RO
Korean Circulation Journal 1998;28(6):953-960
BACKGROUND: It has been well known that the bone and kidney are the principle organs of parathyroid hormine (PTH) actions. Although patients with primary hyperparathyroidism show a high incidence of LVH and trophic effects of PTH on adult rat ventricular cardiomyocytes were investigated in vitro, effect of PTH on the cardiac tissue in vivo is unknown. METHODS: We examined the effects of PTH on the cardiomyocyte and interstitial tissue using adult rat heart. Twenty-two female Sprague-Dawley rats were ovariectomized bilaterally at three months old and weighing in 250 - 300 gm in order to exclude the trophic effect of estrogen. We administrated human parathyroid hormone (20 ug subcutaneously 5 times per week) to 12 rats for 4 weeks after raising for 8 weeks (PTH group):the remaining 10 rats received only normal saline (control). We measured left ventricular thickness [IVS+LVPW)/2] and number of cardiomyocytes and interstitial fibrosis on LM (H & E and Masson's trochrome stain) and EM. RESULTS: 1) LV wall thickeness tended to increase in PTH group as compared with control (2.16+/-0.31 vs 1.12+/-0.21 mm, p=0.099). 2) The number of cardiomyocyte in PTH group was significantly less than that of control (61.2+/-13.1 vs 70.5+/-14.9, p=0.003, Magnification x 400). 3) There was no significant change of interstitial fibrosis between PTH group and control. CONCLUSION: These results shggest that PTH may produce left ventricular hypertrophic effects in aged ovariectomized rat that resulted form hypertrophy of cardiomyocyte without increase of interstitial connetive tissue.
Adult
;
Animals
;
Estrogens
;
Female
;
Fibrosis
;
Heart
;
Humans
;
Hyperparathyroidism, Primary
;
Hypertrophy
;
Hypertrophy, Left Ventricular*
;
Incidence
;
Kidney
;
Myocytes, Cardiac
;
Ovariectomy
;
Parathyroid Hormone*
;
Rats*
;
Rats, Sprague-Dawley
8.The effects of interceed (TC7) on the prevention of adhesion in artificially injured uterus of rabbits.
Yong LEE ; Kwan Sik KIM ; Kyoung Bae PARK ; Seok Mun AHN ; Eun Young YOU ; Hyo Sub RHO ; Soo Kyeong HWANG ; Chull Hee RHYEU ; Jae Kyun DOO ; Jong Duk KIM
Korean Journal of Obstetrics and Gynecology 1993;36(10):3613-3625
9.The effects of interceed (TC7) on the prevention of adhesion in artificially injured uterus of rabbits.
Yong LEE ; Kwan Sik KIM ; Kyoung Bae PARK ; Seok Mun AHN ; Eun Young YOU ; Hyo Sub RHO ; Soo Kyeong HWANG ; Chull Hee RHYEU ; Jae Kyun DOO ; Jong Duk KIM
Korean Journal of Obstetrics and Gynecology 1993;36(10):3613-3625
10.The Relationship between the Leptin Concentration and the Small Dense Low Density Lipoprotein Cholesterol Concentration in Korean Type 2 Diabetic Patients.
Wan Sub SHIM ; Hae Jin KIM ; Eun Seok KANG ; Yu Mie RHEE ; Chul Woo AHN ; Sung Kil LIM ; Hyun Chul LEE ; Bong Soo CHA
Journal of Korean Society of Endocrinology 2006;21(4):319-327
BACKGROUND: Leptin has been suggested as a possible cause of atherosclerotic disease. The small dense low-density lipoprotein cholesterol (LDL-C) has also been regarded as a new surrogate marker in atherosclerotic disease. The aim of this study was to evaluate the relationship between the leptin concentration and the small dense LDL-C concentration in Korean type 2 diabetic patients. METHODS: One hundred-ninety one type 2 diabetic patients, who did not use any medication that could affect the concentration of lipid such as statin, fibrate, thiazolidinediones and corticosteroid, were enrolled in this study. We analyzed the relationship between leptin, the small dense LDL-C and the other metabolic parameters. RESULTS: The small dense LDL-C concentrations were higher in the group with the highest tertile of the leptin value, both in males and females than those patients in the group with the lowest tertile of the leptin value. The small dense LDL-C concentrations were also higher in the group with the highest tertile of leptin divided by the BMI value both in males and females than those patients in the group with the lowest tertile of the leptin value. The leptin concentration was positively correlated with the small dense LDL-C, total cholesterol, triglyceride, LDL-C, insulin and HOMAIR values after adjusting for age, gender and BMI. CONCLUSION: The association between leptin and small dense LDL-C could be a factor that explains the association between leptin and cardiovascular disease.
Biomarkers
;
Cardiovascular Diseases
;
Cholesterol
;
Cholesterol, LDL*
;
Diabetes Mellitus, Type 2
;
Female
;
Humans
;
Hydroxymethylglutaryl-CoA Reductase Inhibitors
;
Insulin
;
Leptin*
;
Lipoproteins
;
Male
;
Thiazolidinediones
;
Triglycerides