The alterations in the localization of keratinocyte membrane glycoconjugates in allergic contact dermatitis were investigated in guinea pig skin treated with topical application of 2.4-dinitro-chlorobenzene. We employed the avidin-biotin complex(ABC) method for the detection of localization of 10 commercially available lectins labelled with biotin: Con-A, SBA, WGA, DBA, UEA-1, RCA-1, PNA, HP, MPA, and ECA. Staining with WGA showed a remarkably decreased intensity in basal and spinous layers of the allergic skin in comparison to those of the control skin, suggesting loss of terminal sialic acids in cell membrane glycoconjugates. The other lectins showed no remarkable difference in the staining patterns between the normal and the allergic ski. The results suggest that epidermal cell membrane glycoconjugates undergoes selective perturbations in acute allergic contact dermatitis, and that the keratinocytes might be an active part of the cutaneous immune system.

BACKGROUND: Cardiopulmonary bypass during open heart surgery causes systemic inflammatory respose. IL-10 is an anti-inflammatory cytokine that inhibits inflammatory process and protects organ function by down regulation of pro-inflammatory cytokine release and maintenance of blood level balance with pro-inflammatory cytokines. MATERIAL ateial and Method: Plasma IL-10 levels were measured and analyzed in 22 patients who underwent open heart surgery (11 cases of coronary artery bypass graft, 11 cases of valve replacement) under cardiopulmonary bypass since 1988 January to July at Department of Thoracic and Czardiovascular surgery, Yeungnam University Hospital. 1g of methylprednisolone was administrated to thirteen patients randomly. Blood samp.es were taken and collected at the time of induction of anesthesia, 10 min before cardiopulmonary bypass, 10 min after starting of CPB, 10 min aftr aortic cross clamping, 10 min after ACC release, and 10 min, 2 hours, and 5 hours after CPB respectively. The plasma levels of IL-10 were determined by enzyme-linked immunosorbent assays (ELISA). Wilcoxon-Raule Sum test was used for statistical analysis. In all 22 patients, cardiopulmonary bypass time was used for statistical analysis. In all 22 patients, cardiopulmonary bypass time was 171+/-41.4 min and aortic cross clamp time was 118+/-36.5 min. Peak IL-10 level was achieved at 10 min after ACC (361.0+/-52.81pg/ml) and was decreased sharply at 2 hours after CPB. Peak IL-10 level was correlated positively with aortic cross clamp time (p=0.011); however, it did not correlated with bypass time (p=0.181). In valve replacement group, mean IL-10 level at peak point was 567.89+/-107.69 pg/ml and was significantly higher than that of coronary artery bypass group (205.67+/-192.70 pg/ml) (p<0.001). ACC time in valve replacement group was significantly longer than that of coronary artery bypass group (p<0.01), however, bypass time was not (p=0.212). Thirteen patients with steroid pretreatment before starting of CPB showed relatively higher plasma IL-10 level than in control group, however, no statistical significance was noted (p=0.19). CONCLUSION: plasma level of IL-10 was increased in association with cardiopulmonary bypass and revealed peak at 10 min after ACC release. IL-10 level was correlated positively with ACC time. Therefore, systemic inflammatory respeonse in association with cardiopulmonary bypass could be decreased by reducing ACC time during cardiac surgery.
Anesthesia ; Cardiopulmonary Bypass* ; Constriction ; Coronary Artery Bypass ; Cytokines ; Down-Regulation ; Enzyme-Linked Immunosorbent Assay ; Humans ; Interleukin-10* ; Methylprednisolone ; Plasma ; Thoracic Surgery ; Transplants

A case of clear cell adenocarcinoma arising in the female urinary bladder, which is accompanied by endometriosis of the urinary bladder and the uterus, is reported. The carcinoma protruded into the vesical lumen as a fungating mass, and had a tubulocyotic pattern. The tumor cell had intracytoplasmic glycogen and electron microscopically short microvilli on their surface, resembling clear cell acenocarcinoma of the female genital tract including ovary. This is the fourth case report of clear cell adenocarcinoma complicating vesical endometriosis, and may support the view that clear cell carcinome arises from endometriosis which, in turn, from the Mullerian remnant.
Female ; Humans ; Adenocarcinoma

A case of clear cell adenocarcinoma arising in the female urinary bladder, which is accompanied by endometriosis of the urinary bladder and the uterus, is reported. The carcinoma protruded into the vesical lumen as a fungating mass, and had a tubulocyotic pattern. The tumor cell had intracytoplasmic glycogen and electron microscopically short microvilli on their surface, resembling clear cell acenocarcinoma of the female genital tract including ovary. This is the fourth case report of clear cell adenocarcinoma complicating vesical endometriosis, and may support the view that clear cell carcinome arises from endometriosis which, in turn, from the Mullerian remnant.
Female ; Humans ; Adenocarcinoma

Infantile myofibromatosis with systemic involvement is a very rare disease and is characterized by numerous nodules composed of spindle cells of a myofibroblastic nature. There are often disseminated throughout the subcutis, muscle, skeleton and viscera. We report an autopsy case of infantile myofibromatosis in a stillborn female fetus of 32 weeks of gestation. The nodules, Imm to 2 cm, were found over the whole body and viscera. The involved viscera were the heart, tongue, esophagus, gastrointestinal tract, portal areas of the liver, spleen anc pancreas. There were also associated malformations, viz., frontal meningoencephalocele, flexion defer-mities, syndactyly, cleft palate, agenesis of corpus callosum, pachygyria, diaphragmatic hemia, renal hypoplasia, etc. Multiple basaloid follicular hamartomas of the skin were noted on the face and extremeties. There are no previous reports in the literature of infantile myofibromatosis in conjunction with the above skin lesion and congenital malformations.
Infant ; Male ; Female ; Humans ; Hamartoma

BACKGROUND: Androgenetic alopecia is considered to be a genetically determined disorder influenced by age and androgen. The proportion of patients with androgenetic alopecia among the total number of patients with alpecia seems to be gradually increasing. OBJECTIVE: The purpose of this study is to determine the family history, clinical and endocrine status of the patients with androgenetic alopectia. METHODS: 387 patients with androgenetic alopecia who had visited the Department of Dermatology, Yongsan Hospital, College of Medicine, Chung-Ang University during the last 4 years (1990.1-1993.12)were examined. RESULTS: 1) Annual incidence of androgenetic alopecia among the total number of alopecia patients averaged over 4 years was 38.95 having increased recently. 2) There were 286 male and 101 female patients being most prevalent in the 3rd decade in both sexes. 3) Patients younger than 30 years old, patients with premature androgenetic alopecia, made up 687.5% of the male patients and 56.4 of the female patients with androgenetic alopecia. 4) Of the 73 male patients Hamilton's type III was most common (46, 63.0%). 5) Of the 34 female patients Ludwig's type I was most common (21, 61.2%). 6) there was a family history of baldness in 39.4% of first degree relatives in male patients and 41.25 of first degree relatives in female patients. 7) Associated diseases were observed in 152 (53.1%) of the male patients and 63(62.4%) of the female patients : seborrheic dermatitis (36.7%/35.7%), acne vulgaris (5.2%/5.95), atopic dermatitis, thyroid diseases, pulmonary tuberculosis, depression, and gastrointestinal diseases. In female patients menstrual irregularity, hypertrichosis, and polycystic ovary disease were also observed. 8) Serum testosterone levels were increased in 24 (8.8%0 of male patients and in 17 (17.45) of female patients. CONCLUSION: Based on our findings, the development of androgenetic alopecia is significantly related to the positive family history of baldness. Serum testosterone levels in most patients were within normal limit and were increased only in a small number of the patients with androgenetic alopecia. Therefore, it seems reasonable to suggest that the inhibitory effecfts of androgens on the dermal papilla cells may be mediated by an increased density and/or functional activity of androgen receptors or through an acceleration in the metabolic activity of androgen.
Acceleration ; Acne Vulgaris ; Adult ; Alopecia* ; Androgens ; Depression ; Dermatitis ; Dermatitis, Atopic ; Dermatitis, Seborrheic ; Dermatology ; Female ; Gastrointestinal Diseases ; Humans ; Hypertrichosis ; Incidence ; Male ; Ovary ; Receptors, Androgen ; Testosterone ; Thyroid Diseases ; Tuberculosis, Pulmonary

The authors eneountered a 39 years old male patient with extensive Fordyce's disease involving the upper lip and buccal mucous mernbrane. The skin lestens were small, discrete and grouped yellowish white papules of about 10 years duration. Diagnosis was made by the characteristic clinical and histopatholqgiC findings.
Adult ; Diagnosis ; Humans ; Lip ; Male ; Skin

The four tetrameric STRs loci(HUMvWA31, HUMTHO1, HUMF13A1, HUMFES/FPS) were studied to confirm the allele frequency distribution and to see whether these results can be used for identity and paternity testing in a population o Koreans using multiplex PCR and laser-fluorescence detection method. In the Korean population (n=227), 8 alleles with their relative frequency range of 0.002-0.249 are detected in the HUMvWA31 locus, 6 alleles with those of 0.007-0.500 in 6 alleles with those of 0.004-0.434 in the HUMFES/FPS locus. The highest observed heterozygosity is found at the locus HUMvWA31(0.8077), with those of the lociively. All loci meet Hardy-Weinberg expectations ; there are good agreements between observed and expected heterozygosity, number of observed genotypes. Pairwise comparisons between loci show allelic independence for all the 4 loci. The power of discrimination (PD) determined for the locus HUMvWA31 is 0.933, that for the HUMTHO1 is 0.836, 0.798 for HUMF13A1, and 0.844 for the HUMFES/FPS ; the combined power of discrimination for the quadruplex is 0.9997. Thus, these allelic frequency distribution can be used to construct the database of the multiplex PCR-based DNA profile in the Korean population. The calculated parameter, "combined power of discrimination(PD)" show the informativeness of these loci for the determination of identity and relatedness of individuals.
Alleles ; Discrimination (Psychology) ; DNA ; Gene Frequency ; Genotype ; Microsatellite Repeats* ; Multiplex Polymerase Chain Reaction* ; Paternity

PURPOSE: This descriptive study was conducted to identify the level of knowledge of stroke symptoms and risk factors among older adults. METHODS: A total of 200 older adults over 65 years of age were conveniently recruited from out patient departments of two hospitals and a health care center from October to November 2008. The level of knowledge was assessed using both open-ended questions and a structured questionnaire based on semi-structured interviews. Data were analyzed by t-tests and ANOVA using the SPSS program. RESULTS: 52.5% of the sample had hypertension and 30% had diabetes. The mean knowledge scores for symptoms and risk factors were 8.4 +/- 3.1(out of 15) and 9.5 +/- 3.9 (out of 16), respectively. The older adults who had lower education, lower family income, and who lived in rural areas were more likely to have less knowledge of stroke symptom and risk factors(p < .05). There was no significant knowledge difference between the older adults who had at least one risk factor and those who had no risk factor for stroke. CONCLUSIONS: Educational intervention should be focused on informing older adults who are at risk for stroke about the early symptoms and management of risk factors, especially those who have low education and low social status.
Adult ; Delivery of Health Care ; Humans ; Hypertension ; Risk Factors ; Stroke ; Surveys and Questionnaires

PURPOSE: The purpose of this study was to investigate the levels in student nurse of knowledge, compliance and risk factor recognition for needlestick injuries. METHOD: Nine hundred and thirty eight(938) student nurse from 3 universities and 3 junior colleges participated in this study. Completed questionnaires were collected between October and November 2004. They were analyzed by using the descriptive statistics and chi2-test, t-test with the SAS program. RESULTS: There were no significant differences in the general characteristics of participants between the two groups - Needlestick Injury(NSI) group and non-Needlestick Injury(non-NSI) group. The scores for knowledge levels of treatment after needlestick injuries and the risk factor recognition level were significantly higher in the NSI group. The scores for performance level as to handling and using needles after needlestick injuries were significantly higher in the non-NSI group. CONCLUSION: It is necessary to develop a preventive program to decrease the needlestick injury rate among student nurse.
Compliance* ; Humans ; Needles ; Needlestick Injuries* ; Surveys and Questionnaires ; Risk Factors*