1.The Differential expression of matrix metalloproteinase and their tissue inhibitors in myometrium and leiomyoma.
Eun Hee YOO ; Eun Mi WHANG ; Han Ki YU ; Seung Bo KIM
Korean Journal of Obstetrics and Gynecology 1999;42(12):2806-2814
OBJECTIVES: To determine the level of mRNA expression of various members of the matrix metalloproteinase and tissue inhibitors in uterine leiomyoma compared with unaffected myometrium. Materials & Method: 30 cases of portions of leiomyoma and myometrium were collected immediately followimg hysterectomy. Thirteen cases were from proliferative phase and seventeen were from secretory phase of menstrual cycle. The mean age was 43.7years old. The level of expression of mRNAs of interstitial collagenase, gelatinase, stromelysin, TIMP-1,-2,-3 was determined by reverse transcriptase-polymerase chain reaction(RT-PCR) and normalized to GAPDH(glyceraldehyde-3-phosphate dehydrogenase) mRNA. RESULTS: Myometrium and leiomyoma expressed all the members of above mentioned matrix metalloproteinase family and tissue inhibitors. Leiomyoma expressed a significantly higher level of stromelysin-3 during secretory phase, an extremely lower level of 92kDa gelatinase and a significantly lower level of TIMP-3. The immunohistochemical localization of TIMP-3 was smooth muscle cell and arteriole wall of myometrium and leiomyoma. CONCLUSIONS: The increased expression of stromelysin-3 in uterine leiomyoma compared with myometrium suggests that this MMP may be involved in the formation of a more fibrous extracellular matrix in leiomyoma. The extremely lower expression of 92kDa gelatinase of leiomyoma means that leiomyoma do not invade myometrium and forms a separated mass. Decreased expression of TIMP-3 of leiomyoma suggests that TIMP-3 is required for differentiation and homeostasis of extracellular matrix of normal myometrium and function as a suppressive role of tumor development
Animals
;
Arterioles
;
Extracellular Matrix
;
Female
;
Gelatinases
;
Homeostasis
;
Humans
;
Hysterectomy
;
Leiomyoma*
;
Matrix Metalloproteinase 1
;
Matrix Metalloproteinase 3
;
Menstrual Cycle
;
Mice
;
Myocytes, Smooth Muscle
;
Myometrium*
;
RNA, Messenger
;
Tissue Inhibitor of Metalloproteinase-3
2.Bronchiolo-alveolar cell carcinoma a review of 11 cases
Sook Ran MOON ; Eun Mi CHUNG ; Chang Joon LEE ; In Soon WHANG ; Han Suk KIM
Journal of the Korean Radiological Society 1983;19(2):339-346
Eleven patients with proved Bronchiolo-aveolar cell carcinoma were found in the chest department of thenational menical center from 1975 to 1981. The incidence of Bronchiolo-alveolar cell carcinoma is recentlyincreased as primary lung carcinoma. The result as follow. 1. The ratio of male and female was 5:6 and anincidence of 4.4% among total primary lung cancer patients. The highest incidence (3 of cases) was seen in thesixth decade, and the remaining cases were evenly distributed in the third, fourth, and fifth decades of life.Among them youngest was 29 years old and the oldest was 66 years old. 2. Clinical and radiological initialdiagnosis prior to the final diagnosis were as follows; pulmonary tuberculosis; 7 cases, pneumonia; 1 case,bronchiectasis; 1 case, and lung cancer; 2 cases. 3. Radiological examination of chest presented several pictures;most commonly, homogenous or patchy infiltrations; 6 cases, nodular or mass like densities; 2 cases, disseminatednodular or military patterns; 2 cases, and reticular pattern; 1 case. 4. Bronchogram reveald no contributablefindings except one case of complete tappering obstruction of the segmental bronchus. Therefore we arrive at theconclusion that early diagnosis will result in increased resectability and improved survival so aggressivediagnositic work-up for suspicious pulmonary infiltrate is necessary.
Bronchi
;
Diagnosis
;
Early Diagnosis
;
Female
;
Humans
;
Incidence
;
Lung
;
Lung Neoplasms
;
Male
;
Military Personnel
;
Pneumonia
;
Thorax
;
Tuberculosis, Pulmonary
3.The expression of NCAM ( Neural Cell Adhesion Molecule) in myometrium and leiomyoma.
Eun Hee YOO ; Eun Mi WHANG ; Hye Sung MOON ; Hae Soo KOO ; Jong Il KIM ; Han Ki YU
Korean Journal of Obstetrics and Gynecology 2000;43(10):1808-1812
No abstract available.
Animals
;
Cell Adhesion*
;
Female
;
Leiomyoma*
;
Mice
;
Myometrium*
;
Neural Cell Adhesion Molecules*
4.Absence of Human Papillomavirus DNA in Nongenital Seborrheic Keratosis.
Eun So LEE ; Mi Ran WHANG ; Won Hyoung KANG
Journal of Korean Medical Science 2001;16(5):619-622
Seborrheic keratosis (SK) is a benign epidermal tumor of unknown etiology. Because of its wart-like morphology, human papillomavirus (HPV) has been suggested as a possible causative agent. Viral involvement, however, has not been confirmed yet despite extensive research. The aim of this study was to evaluate the presence of HPV 6/11, 31, 33 DNA in nongenital SK. We analyzed 40 biopsy specimens taken from patients with nongenital SK using in situ polymerase chain reaction (PCR) and PCR with tissue extracts. The SK specimens (n=40), analyzed by in situ PCR, were negative for all HPV probes tested (types 6/11, 31, 33). Control slides (condyloma acuminatum, n=3) were positive for type 6/11, 31, and 33 HPV probes tested. Melasma samples (n=4), the negative controls, were consistently negative. No HPV DNA band was detected by PCR with the tissue extracts from paraffin-embedded SK samples, while condyloma acuminatum, the positive controls, showed DNA bands of the correct molecular weights. Our results show that HPV type 6/11, 31, and 33 cannot be recognized as causative agents for nongenital SK, which is in contrast to the previous studies. Further studies are required to reveal the presence of other types (more than 90) of HPV DNA.
DNA, Viral/*analysis
;
Female
;
Human
;
Keratosis, Seborrheic/*virology
;
Male
;
Papillomavirus, Human/*isolation & purification
;
Polymerase Chain Reaction
5.Hysterosalpingographic evaluation in infertility
Jin Sik KIM ; Marie SHIN ; Eun Mi JUNG ; Chong Hyun YOON ; Chang Joon LEE ; In Soon WHANG ; Han Suk KIM
Journal of the Korean Radiological Society 1984;20(4):895-901
H.S.G. has been proven to be an important diagnsotic method in clinical gyencology for under 50 yrs. It isvaluable in the investigation of the uterine and tubal factors of female infertility. Hysterosalpingograms of 81 patients with infertility were analysed and following brief results were obtained. 1. Ratio between primary andsecondary infertility was 3:5, 2ndary infertility was more frequent. 2. Age distribution was more frequnet under 30 years of age than over 30 years of age. 3. Abnormal uterine finding was only seen in 18 cases, abnormal tubalfinding was only seen in 25 cases and combined uterine and abnormalities were seen in 14 cases. 4. Abnormaluterine findings were malposition (12 cases), filling defect (5), spastic uterus(5), irregular contour (3),arcuate uterus(3), dideplphia(1), in order of frequency. 5. Abnormal tubal findings were hydrosalpinx (21 cases), occlusion(19), intravasation (6), beaded appearance(3), & diverticula(2) , in order of frequency. 6. Negativefinding in both uterus and tube was seen in 23 cases. 7. In 2 cases, pregnancy after the H.S.G. examination wasconfirmed.
Age Distribution
;
Female
;
Humans
;
Infertility
;
Infertility, Female
;
Methods
;
Muscle Spasticity
;
Pregnancy
;
Uterus
6.Usefulness of Refractive Measurement by Wavefront Aberrometer in Children
Si Eun OH ; Woong Joo WHANG ; Mi Ra PARK
Journal of the Korean Ophthalmological Society 2021;62(5):680-687
Purpose:
To compare the refractive measurements from a wavefront aberrometer, autorefractor, and retinoscopy after cycloplegia in evaluating the usefulness and validity of refractive measurements by a wavefront aberrometer in children.
Methods:
A total of 130 eyes of 65 children, aged from 3 to 16 years, were examined using retinoscopy, a wavefront aberrometer (OPD-Scan III), and an autorefractor (KR-1) after cycloplegia. Refractive measurements were converted to power vectors (M, J0, and J45) and cylindrical absolute values for statistical analysis. The agreement between instruments was assessed and the correlations of measurements were evaluated. Subgroup analysis was performed on two subgroups: one representing less refractive error (|M| < 2 D on cycloplegic retinoscopy) and the other with larger refractive error (|M| ≥ 2 D on cycloplegic retinoscopy).
Results:
Compared with retinoscopy readings, the aberrometer and autorefractor yielded more myopic values (p = 0.007, p < 0.001). In the less refractive error group, the autorefractor results showed statistically significant differences from retinoscopy readings for M, J0, and J45 and the cylindrical absolute value (all p < 0.05); there were no statistically significant differences between M, J0, and J45 vectors of the aberrometer and those obtained using retinoscopy (p = 0.674, p = 0.699, p = 0.766). With the larger refractive error group, the M vectors of the aberrometer and autorefractor showed more myopic values than the M vector retinoscopy readings; the differences were statistically significant (all p < 0.001).
Conclusions
The wavefront aberrometer yielded refraction readings closer to those obtained with retinoscopy than the automated refraction in the less refractive error group. With a larger refractive error, statistically significant differences (all p < 0.001) were found among the aberrometer, autorefractor, and retinoscopy readings.
7.Usefulness of Refractive Measurement by Wavefront Aberrometer in Children
Si Eun OH ; Woong Joo WHANG ; Mi Ra PARK
Journal of the Korean Ophthalmological Society 2021;62(5):680-687
Purpose:
To compare the refractive measurements from a wavefront aberrometer, autorefractor, and retinoscopy after cycloplegia in evaluating the usefulness and validity of refractive measurements by a wavefront aberrometer in children.
Methods:
A total of 130 eyes of 65 children, aged from 3 to 16 years, were examined using retinoscopy, a wavefront aberrometer (OPD-Scan III), and an autorefractor (KR-1) after cycloplegia. Refractive measurements were converted to power vectors (M, J0, and J45) and cylindrical absolute values for statistical analysis. The agreement between instruments was assessed and the correlations of measurements were evaluated. Subgroup analysis was performed on two subgroups: one representing less refractive error (|M| < 2 D on cycloplegic retinoscopy) and the other with larger refractive error (|M| ≥ 2 D on cycloplegic retinoscopy).
Results:
Compared with retinoscopy readings, the aberrometer and autorefractor yielded more myopic values (p = 0.007, p < 0.001). In the less refractive error group, the autorefractor results showed statistically significant differences from retinoscopy readings for M, J0, and J45 and the cylindrical absolute value (all p < 0.05); there were no statistically significant differences between M, J0, and J45 vectors of the aberrometer and those obtained using retinoscopy (p = 0.674, p = 0.699, p = 0.766). With the larger refractive error group, the M vectors of the aberrometer and autorefractor showed more myopic values than the M vector retinoscopy readings; the differences were statistically significant (all p < 0.001).
Conclusions
The wavefront aberrometer yielded refraction readings closer to those obtained with retinoscopy than the automated refraction in the less refractive error group. With a larger refractive error, statistically significant differences (all p < 0.001) were found among the aberrometer, autorefractor, and retinoscopy readings.
8.Rapidly Growing Bilateral Pseudoangiomatous Stromal Hyperplasia of the Breast.
Eun Mi RYU ; In Yong WHANG ; Eun Deok CHANG
Korean Journal of Radiology 2010;11(3):355-358
A tumoral pseudoangiomatous stromal hyperplasia (PASH) that causes huge breast enlargement is very rare. Only two cases of huge tumoral PASHs have been reported in the English medical literature. We report here on a surgically confirmed case of bilateral huge tumoral PASH in a 47-year-old woman, and we present the imaging and histopathology findings. We also review the relevant medical literature.
Angiomatosis/*pathology/surgery/ultrasonography
;
Biopsy, Needle
;
Breast/cytology/pathology/surgery
;
Breast Diseases/*pathology/surgery/ultrasonography
;
Contrast Media/diagnostic use
;
Diagnosis, Differential
;
Female
;
Gadolinium DTPA/diagnostic use
;
Humans
;
Hyperplasia
;
Image Enhancement/methods
;
Magnetic Resonance Imaging/methods
;
Mammography/methods
;
Middle Aged
;
Stromal Cells/pathology
9.Eosinophilic Enteritis Involving the Entire Intestinal Wall Presenting as Small Bowel Obstruction and Acute Abdomen.
Hang Joo CHO ; Young Mi KU ; In Yong WHANG ; Kyoung Ho CHOI ; Eun Jung LEE ; Chang Hyeok AN
Journal of the Korean Society of Emergency Medicine 2010;21(5):717-719
Eosinophilic enteritis is an uncommon disease that rarely manifests as an acute abdomen. A 50-year-old man visited our hospital and complained of epigastric and periumbilical pain. He reported direct and rebound tenderness over the periumbilical area. Laboratory examinations showed leukocytosis without eosinophilia. Plain abdominal radiography revealed air-fluid levels in the small intestine and computed tomography revealed concentric wall thickening in the small bowel and ascites in the pelvic cavity. We conducted an emergency operation and segmental resection of the jejunum was performed. Histologically, transmural, eosinophilic infiltration was observed. In patients with intestinal obstruction, even when presenting with an acute abdomen with no peripheral eosinophilia, eosinophilic enteritis should be included in the differential diagnosis.
Abdomen, Acute
;
Ascites
;
Diagnosis, Differential
;
Emergencies
;
Enteritis
;
Eosinophilia
;
Eosinophils
;
Gastritis
;
Gastroenteritis
;
Humans
;
Intestinal Obstruction
;
Intestine, Small
;
Jejunum
;
Leukocytosis
;
Middle Aged
;
Radiography, Abdominal
10.Body Composition by Bioelectrical Impedance Analysis in Obese Children.
Jong Eun LEE ; Ki Won PARK ; Su Jin CHO ; Il Tae WHANG ; Young Mi HONG
Journal of the Korean Pediatric Society 2001;44(9):992-1001
PURPOSE: Body composition by bioelectrical impedance analysis(BIA) is a very useful method of analysing body composition in many diseases. BIA is a simple, rapid, non-invasive and reproducible technique. There was no comparative analysis between obese and healthy children. A comparative analysis between obese and healthy children is needed to assess the effect of excercise and other interventions in the management of childhood obesity. This study is designed to analyze and compare the body composition and body fat percent measured by BIA in healthy and obese children. METHODS: Height and weight were measured. BIA was done in healthy and obese children. Body compositions were analyzed with fat mass, lean body mass, body fat percent, protein, mineral, intracellular fluid(ICF), and extracellular fluid(ECF). RESULTS: Fat mass, fat percent, ICF, and ECF were significantly higher in the obese group. Resistances were significantly lower in the obese group. The amounts of ICF and ECF were significantly higher in obese group. Fat mass showed more significant correlation with ICF and ECF than body mass index. CONCLUSION: Body composition analysis using bioelectrical impedance(BI) is considered as very useful diagnostic method in the evaluation and treatment in childhood obesity. Epidemiologic study will be needed to determine the diagnostic criteria of obesity by BI.
Adipose Tissue
;
Body Composition*
;
Body Mass Index
;
Child*
;
Electric Impedance*
;
Epidemiologic Studies
;
Humans
;
Obesity
;
Pediatric Obesity