1.Quantification of Hepatitis C Virus RNA in Patients Sera by Competitive RT-nested PCR.
Sae Gwang PARK ; Eun Young JE ; Jae Eun PAIK ; Hee Kyung OH ; In Hak CHOI
Journal of the Korean Society for Microbiology 2000;35(5):351-351
No Abstract Available.
Hepacivirus*
;
Hepatitis C*
;
Hepatitis*
;
Humans
;
Polymerase Chain Reaction*
2.Quantification of Hepatitis C Virus RNA in Patients Sera by Competitive RT-nested PCR.
Sae Gwang PARK ; Eun Young JE ; Jae Eun PAIK ; Hee Kyung OH ; In Hak CHOI
Journal of the Korean Society for Microbiology 2000;35(5):351-351
No Abstract Available.
Hepacivirus*
;
Hepatitis C*
;
Hepatitis*
;
Humans
;
Polymerase Chain Reaction*
3.Lymphocyte proliferation and antibody response against 30-kDa protein antigen of mycobacterium tuberculosis.
Tae Hyun PAIK ; Bong Kyu LEE ; Hwa Jung KIM ; Eun Gyeong JO ; Tae Kyung CHOI
Journal of the Korean Society for Microbiology 1992;27(3):253-268
No abstract available.
Antibody Formation*
;
Lymphocytes*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
4.Determination of Anti-tuberculous Antibody Against Triton X-100 Solubilized Protein ( TSP ) Antigen of Mycobacterium tuberculosis in the Sera of Patients with Pulmonary Tuberculosis.
Tae Hyun PAIK ; Jeong Kyu PARK ; Hwa Jung KIM ; Kyung Jin KIM ; Eun Kyeong JO
Journal of the Korean Society for Microbiology 1997;32(1):59-70
Some of the proteins of mycobacteria are preferentially associated with the cell wall and are powerful immunogens, and humoral antibody responses to these mycobacterial antigens may occur in patients with tuberculosis. In this study, Triton X-100 solubilized protein (TSP) antigen was isolated from Mycobacterium tuberculosis H37Rv by overnight shaking with 1% Triton X- 100/PMSF and 10-90% ammonium sulfate precipitation. IgG and IgM antibody levels against TSP, crude protein from the unheated cultrue filtrate (CF#) and 30 kDa antigens were determined in the sera of 80 patients with pulmonary tuberculosis and 99 healthy controls with PPD (+) and (-). High IgG reactivity to TSP and CF antigen was observed in tuberculosis patients. Mean IgG antibody titers against all of three mycobacterial antigens were differed significantly (P<0.01) between patients and controls but IgM showed no difference. By the cut-off value adding 2 standard deviation to the mean absorbance of controls, the sensitivity and specificity of the IgG antibody to TSP antigen were 93.9% and 77.5%. The specificity to TSP antigen was a litttle higher than those obtained by CF and 30 kDa antigen. From the above results, the TSP antigen may be useful for the serodiagnosis of tuberculosis.
Ammonium Sulfate
;
Antibody Formation
;
Cell Wall
;
Humans
;
Immunoglobulin G
;
Immunoglobulin M
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Neptune*
;
Octoxynol*
;
Sensitivity and Specificity
;
Serologic Tests
;
Tuberculosis
;
Tuberculosis, Pulmonary*
5.Ureteral Fibroepithelial Polyp: A report of four cases (One case with nephrogenic adenoma).
Won Mee LEE ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1996;30(8):715-720
Fibroepithelial polyps of the ureter are usually acquired rather than congenital. Most polyps are hamartomatous growths which tend to arise in the proximal portion of the left ureter. Most patients exibit either hematuria or persistent flank pain secondary to partial ureteral obstruction. Because of false positivity of urine cytology, as well as intravenous pyelogram, the correct diagnosis is confused with malignancy. Herein we report four cases of ureteral fibroepithelial polyp, which are associated with stones resulting in partial obstruction of the ureter. One of the four cases is associated with nephrogenic adenoma in the lamina propria of the adjacent ureter. The following report describes clinicopathologic findings of fibroepithelial polyp with review of literatures.
Adenoma
6.Idiopathic Entero-colic Lymphocytic Phlebitis: A case report.
Seung Sam PAIK ; Young Ha OH ; Eun Kyung HONG ; Jung Dal LEE
Korean Journal of Pathology 1996;30(6):533-538
Localized enterocolic lymphocytic phlebitis is characterized by selective phlebitis involving the small to medium-sized veins and venules, infiltration exclusively by lymphocytes, and no other systemic vasculitis or inflammatory bowel disease. This vasculitis can be a rare cause of intestinal ischemia. We experienced a case of enterocolic lymphocytic phlebitis in a 72-year-old woman, who presented with abdominal pain and distension. The resected colon and terminal ileum showed striking lymphocytic phlebitis affecting the veins and venules of the bowel and mesentery which resulted in ischemic injury of the bowel. This vasculopathy was the only demonstrable cause of ischemia. Arteritis and arteriolitis was not found. There is no clinical or laboratory evidence or a history of extraintestinal vasculitis. The etiology of this clinicopathological entity has not been elucidated. Herein, we report the clinicopathological findings in this patient who presented with ischemic intestinal necrosis caused by localized intestinal lymphocytic phlebitis associated with thrombosis.
Female
;
Humans
7.Detection of mycobacterium tuberculosis in sputum samples by polymerase chain reaction.
Eun Gyeong JO ; Tae Kyung CHOI ; Tae Hyun PAIK ; Jeong Kyu PARK ; Hwa Jung KIM
Journal of the Korean Society for Microbiology 1993;28(2):131-142
No abstract available.
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymerase Chain Reaction*
;
Sputum*
8.Purification of 30-kDa and 32 kDa protein antigens from mycobacterium tuberculosis and activation of human monocytes by lymphokines.
Tae Kyung CHOI ; Hwa Jung KIM ; Eun Gyeong JO ; Jeong Kyu PARK ; Tae Hyun PAIK
Journal of the Korean Society for Microbiology 1993;28(2):113-130
No abstract available.
Humans*
;
Lymphokines*
;
Monocytes*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
9.Ossifying Fibromyxoid Tumor of Soft Parts.
Seok Hoon JEON ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):174-178
An ossifying fibromyxoid tumor of soft parts is a rare, recently described, fibro-osseous neoplasm of uncertain histogenesis. It occurs most frequently within the subcutis or skeletal muscle of the extremities. Its biologic behavior is generally regarded as benign with at worst a locally aggressive clinical course. But, atypical and malignant variants have been recently reported. Herein we report a case of a benign ossifying fibromyxoid tumor which occurred in the left upper back of 41-year-old man. The tumor is composed of uniformly round or polygonal cells arranged in cords or nests which are separated by myxoid and hyalinzed fibrous matrix and associated with irregular bony trabeculae. The tumor cells are strong positive for vimentin. Ultrastructural findings and a review of literatures are added.
Adult
;
Extremities
;
Humans
;
Muscle, Skeletal
;
Vimentin
10.Ossifying Fibromyxoid Tumor of Soft Parts.
Seok Hoon JEON ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):174-178
An ossifying fibromyxoid tumor of soft parts is a rare, recently described, fibro-osseous neoplasm of uncertain histogenesis. It occurs most frequently within the subcutis or skeletal muscle of the extremities. Its biologic behavior is generally regarded as benign with at worst a locally aggressive clinical course. But, atypical and malignant variants have been recently reported. Herein we report a case of a benign ossifying fibromyxoid tumor which occurred in the left upper back of 41-year-old man. The tumor is composed of uniformly round or polygonal cells arranged in cords or nests which are separated by myxoid and hyalinzed fibrous matrix and associated with irregular bony trabeculae. The tumor cells are strong positive for vimentin. Ultrastructural findings and a review of literatures are added.
Adult
;
Extremities
;
Humans
;
Muscle, Skeletal
;
Vimentin