1.In vitro Stimulation of Tumor - Draining Lymph Node Lymphocytes with the 30 kDa Antigen of Mycobacterium tuberculosis Leads to the Differentiation of Th1 Cells and Cytotoxic Effector Cells.
Jeong Kyu PARK ; Tae Hyun PAIK ; Seok Shin KOH ; Hwa Jung KIM ; Eun Kyeong JO
Korean Journal of Immunology 1997;19(1):59-72
Tumor-draining lymph node (TDLN) lymphocytes contain immunologically sensitized to tumor but functionally deficient T cells. The 30 kDa protein antigen, a major secreted protein antigen of Mycobacterium tuberculosis, exhibits strong T cell stimulatory effect. In this study, it examined that the feasibility of using M tuberculosis 30 kDa antigen to stimulate tumor-draining lymph node cells for the generation of specific immune effector cells. Freshly isolated TDLN lymphocytes could directly respond to the 30 kDa antigen alone and their proliferative responses were markedly augmented by stimulation with rIL-2. TDLN cells were stimulated with the 30 kDa antigen for various time intervals and examined for the induction of IFN-r and IL-4 mRNA using RT-PCR. The expression of IFN-r mRNA was greatly augmented after 1 wk, whereas IL-4 mRNA is markedly decreased after 1 wk. Cytotoxic T cell activities induced by the 30 kDa antigen was also evaluated. TDLN cells stimulated with the 30 kDa antigen alone were able to generate remarkable cytotoxic response to K562 or Daudi cell lines after 6 days of culture. And their cytotoxic effects were highly augmented by stirnulation with rIL-2. These results suggest that the 30 kDa antigen of M. tuberculosis may selectively activate Thl cells of TDLN lymhocytes and induce the cytotoxic T cell activities. In conclusion, the 30 kDa antigen can be used as a biologic response modifier in tumor immunology.
Allergy and Immunology
;
Cell Line
;
Interleukin-4
;
Lymph Nodes*
;
Lymphocytes*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
RNA, Messenger
;
T-Lymphocytes
;
Th1 Cells*
;
Tuberculosis
2.Detection of mycobacterium tuberculosis in sputum samples by polymerase chain reaction.
Eun Gyeong JO ; Tae Kyung CHOI ; Tae Hyun PAIK ; Jeong Kyu PARK ; Hwa Jung KIM
Journal of the Korean Society for Microbiology 1993;28(2):131-142
No abstract available.
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Polymerase Chain Reaction*
;
Sputum*
3.Purification of 30-kDa and 32 kDa protein antigens from mycobacterium tuberculosis and activation of human monocytes by lymphokines.
Tae Kyung CHOI ; Hwa Jung KIM ; Eun Gyeong JO ; Jeong Kyu PARK ; Tae Hyun PAIK
Journal of the Korean Society for Microbiology 1993;28(2):113-130
No abstract available.
Humans*
;
Lymphokines*
;
Monocytes*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
4.Ossifying Fibromyxoid Tumor of Soft Parts.
Seok Hoon JEON ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):174-178
An ossifying fibromyxoid tumor of soft parts is a rare, recently described, fibro-osseous neoplasm of uncertain histogenesis. It occurs most frequently within the subcutis or skeletal muscle of the extremities. Its biologic behavior is generally regarded as benign with at worst a locally aggressive clinical course. But, atypical and malignant variants have been recently reported. Herein we report a case of a benign ossifying fibromyxoid tumor which occurred in the left upper back of 41-year-old man. The tumor is composed of uniformly round or polygonal cells arranged in cords or nests which are separated by myxoid and hyalinzed fibrous matrix and associated with irregular bony trabeculae. The tumor cells are strong positive for vimentin. Ultrastructural findings and a review of literatures are added.
Adult
;
Extremities
;
Humans
;
Muscle, Skeletal
;
Vimentin
5.Ossifying Fibromyxoid Tumor of Soft Parts.
Seok Hoon JEON ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):174-178
An ossifying fibromyxoid tumor of soft parts is a rare, recently described, fibro-osseous neoplasm of uncertain histogenesis. It occurs most frequently within the subcutis or skeletal muscle of the extremities. Its biologic behavior is generally regarded as benign with at worst a locally aggressive clinical course. But, atypical and malignant variants have been recently reported. Herein we report a case of a benign ossifying fibromyxoid tumor which occurred in the left upper back of 41-year-old man. The tumor is composed of uniformly round or polygonal cells arranged in cords or nests which are separated by myxoid and hyalinzed fibrous matrix and associated with irregular bony trabeculae. The tumor cells are strong positive for vimentin. Ultrastructural findings and a review of literatures are added.
Adult
;
Extremities
;
Humans
;
Muscle, Skeletal
;
Vimentin
6.Clear Cell Islet Cell Tumor of the Pancreas: An Immunohistochemical and Ultrastructural study.
Seung Sam PAIK ; Young Ha OH ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):162-166
A clear cell islet cell tumor of the pancreas is extremely rare and characterized by extensive clear cell components. Electron microscopic and immunohistochemical findings are essential to prove that the mass with clear cells is an unusual manifestation of an islet cell tumor. Herein, we report a case of clear cell islet cell tumor of a 54-year-old woman with abdominal pain. The tumor was composed of polygonal clear cells arranged in nests, trabeculae, and ribbon pattern with the extensively fibrous stroma. These tumor cells showed strong reactivity for chromogranin and weak reactivity for somatostatin and glucagon. An electron microscope revealed that the important contributing factor of the clear cytoplasmic change was mainly due to an accumulation of lipid droplets, coupled with cytoplasmic swelling in some areas. Some tumor cells showed many endosecretory granules ranging from 111 to 297nm in diameter. In the clinical and immunohistochemical findings these granules were consistent with somatostatin granules in morphology and size.
Abdominal Pain
;
Adenoma, Islet Cell*
;
Cellular Structures
;
Cytoplasm
;
Female
;
Glucagon
;
Humans
;
Islets of Langerhans*
;
Middle Aged
;
Pancreas*
;
Somatostatin
7.Clear Cell Islet Cell Tumor of the Pancreas: An Immunohistochemical and Ultrastructural study.
Seung Sam PAIK ; Young Ha OH ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1997;31(2):162-166
A clear cell islet cell tumor of the pancreas is extremely rare and characterized by extensive clear cell components. Electron microscopic and immunohistochemical findings are essential to prove that the mass with clear cells is an unusual manifestation of an islet cell tumor. Herein, we report a case of clear cell islet cell tumor of a 54-year-old woman with abdominal pain. The tumor was composed of polygonal clear cells arranged in nests, trabeculae, and ribbon pattern with the extensively fibrous stroma. These tumor cells showed strong reactivity for chromogranin and weak reactivity for somatostatin and glucagon. An electron microscope revealed that the important contributing factor of the clear cytoplasmic change was mainly due to an accumulation of lipid droplets, coupled with cytoplasmic swelling in some areas. Some tumor cells showed many endosecretory granules ranging from 111 to 297nm in diameter. In the clinical and immunohistochemical findings these granules were consistent with somatostatin granules in morphology and size.
Abdominal Pain
;
Adenoma, Islet Cell*
;
Cellular Structures
;
Cytoplasm
;
Female
;
Glucagon
;
Humans
;
Islets of Langerhans*
;
Middle Aged
;
Pancreas*
;
Somatostatin
8.Ureteral Fibroepithelial Polyp: A report of four cases (One case with nephrogenic adenoma).
Won Mee LEE ; Seung Sam PAIK ; Eun Kyung HONG ; Moon Hyang PARK ; Jung Dal LEE
Korean Journal of Pathology 1996;30(8):715-720
Fibroepithelial polyps of the ureter are usually acquired rather than congenital. Most polyps are hamartomatous growths which tend to arise in the proximal portion of the left ureter. Most patients exibit either hematuria or persistent flank pain secondary to partial ureteral obstruction. Because of false positivity of urine cytology, as well as intravenous pyelogram, the correct diagnosis is confused with malignancy. Herein we report four cases of ureteral fibroepithelial polyp, which are associated with stones resulting in partial obstruction of the ureter. One of the four cases is associated with nephrogenic adenoma in the lamina propria of the adjacent ureter. The following report describes clinicopathologic findings of fibroepithelial polyp with review of literatures.
Adenoma
9.Lymphocyte proliferation and antibody response against 30-kDa protein antigen of mycobacterium tuberculosis.
Tae Hyun PAIK ; Bong Kyu LEE ; Hwa Jung KIM ; Eun Gyeong JO ; Tae Kyung CHOI
Journal of the Korean Society for Microbiology 1992;27(3):253-268
No abstract available.
Antibody Formation*
;
Lymphocytes*
;
Mycobacterium tuberculosis*
;
Mycobacterium*
10.Determination of Anti-tuberculous Antibody Against Triton X-100 Solubilized Protein ( TSP ) Antigen of Mycobacterium tuberculosis in the Sera of Patients with Pulmonary Tuberculosis.
Tae Hyun PAIK ; Jeong Kyu PARK ; Hwa Jung KIM ; Kyung Jin KIM ; Eun Kyeong JO
Journal of the Korean Society for Microbiology 1997;32(1):59-70
Some of the proteins of mycobacteria are preferentially associated with the cell wall and are powerful immunogens, and humoral antibody responses to these mycobacterial antigens may occur in patients with tuberculosis. In this study, Triton X-100 solubilized protein (TSP) antigen was isolated from Mycobacterium tuberculosis H37Rv by overnight shaking with 1% Triton X- 100/PMSF and 10-90% ammonium sulfate precipitation. IgG and IgM antibody levels against TSP, crude protein from the unheated cultrue filtrate (CF#) and 30 kDa antigens were determined in the sera of 80 patients with pulmonary tuberculosis and 99 healthy controls with PPD (+) and (-). High IgG reactivity to TSP and CF antigen was observed in tuberculosis patients. Mean IgG antibody titers against all of three mycobacterial antigens were differed significantly (P<0.01) between patients and controls but IgM showed no difference. By the cut-off value adding 2 standard deviation to the mean absorbance of controls, the sensitivity and specificity of the IgG antibody to TSP antigen were 93.9% and 77.5%. The specificity to TSP antigen was a litttle higher than those obtained by CF and 30 kDa antigen. From the above results, the TSP antigen may be useful for the serodiagnosis of tuberculosis.
Ammonium Sulfate
;
Antibody Formation
;
Cell Wall
;
Humans
;
Immunoglobulin G
;
Immunoglobulin M
;
Mycobacterium tuberculosis*
;
Mycobacterium*
;
Neptune*
;
Octoxynol*
;
Sensitivity and Specificity
;
Serologic Tests
;
Tuberculosis
;
Tuberculosis, Pulmonary*