1.Detection of Major bcr/abl mRNA from Stored Bone Marrow Aspirate Smears Using the Reverse Transcriptase Polymerase Chain Reaction.
Eun Yup LEE ; Jeong Hwan SHIN ; Eun Sook JUN
Korean Journal of Clinical Pathology 1997;17(4):668-675
BACKGROUND: The verse transcriptase polymerase chain reaction (RT-PCR) has been widely used to analyze the bcr/abl fusion mRNA in chronic myelogenous leukemia (CML). Fresh or cryopreserved cells may not always be available for molecular diagnosis. So we investigated the value of stored bone marrow aspirate smears as the sources of material for the detection of bcr/abl mRNA. METHODS: We extracted RNA using modified Chomczynski method, and amplified bcr/abl mRNA by RT-PCR from the 70 cases of bone marrow smear slides stored from 7 days to 7 years, which were comprised of 49 CML, 11 other chronic myeloproliferative disorders (CMPD) and 10 acute lymphoblastic leukemia (ALL). Sensitivity of RT-PGR was tested using the slide smears prepared with 10(0)-10(6) K562 cells, and RT-PCR results losing each fresh bone marrow cellular suspension and slide smears in 24 patients were compacted. For major bcr/abl rearrangement, RT-PCR was performed by nested PGR afters GDNA synthesis losing downward primer and beta2-microglobulin was used as RNA controls. RESULTS: The sensitivity of RT-PCR for detecting bcr/abl mRNA was l02 cells per slide. Sixty one cases (86%) of 70 bone marrow aspirate smears showed positive results of beta2-micyoglobulin cDNA as an indicator of intact RNA. Thirty nine cases of 42 beta2-microglobulin cDNA positive CML bone marrow aspirate smears showed 29 b3a2 type mRNA and 10 b2a2 type mRNA. Nine cases of 11 bone marrow aspirate smear with other CMPD showed negative results of bcr/abl mRNA. Two cases of 10 ALL bone mallow aspirate smears had b2a2 type mRNA and b3a2 type mRNA, respectively. The results for detection of bcr/abl mRNA with fresh cell suspensions of 24 patients were same as the bone marrow aspirate smears storied for 7 days to 1 year. CONCLUSIONS: These data indicated that RNA obtained from bone marrow smears storied for less than 1 year was valuable as the source of RT-PGR for the detection of bcr/abl mRNA in CML and the bone marrow smears stored for much longer period ould be assailable as the specimens for retrospective analysis of specific gene alter-ation in other hematologic malignancy.
Bone Marrow*
;
Diagnosis
;
DNA, Complementary
;
DNA-Directed RNA Polymerases
;
Hematologic Neoplasms
;
Humans
;
K562 Cells
;
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
;
Myeloproliferative Disorders
;
Polymerase Chain Reaction
;
Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
Retrospective Studies
;
Reverse Transcriptase Polymerase Chain Reaction*
;
RNA
;
RNA, Messenger*
;
RNA-Directed DNA Polymerase*
;
Suspensions
2.A Case of Spontaneous Discitis in Child.
Eun Jung LEE ; Joon Soo LEE ; Chang Jun COE
Journal of the Korean Child Neurology Society 1998;6(1):133-137
The authors present a case of spontaneous discitis in a 1-year-old female who presented with fever and limping gait. The erythrocyte sediment rate was increased. The roentgenograms revealed normal appearnce, and radioactive bne scan showed increased uptake at the affected level. Discitis should be considered in any child with limping gait or leg pain, refusal to walk, or abdominal pain. Early recognition may avoid unnecessary diagnotic and treatment procedures.
Abdominal Pain
;
Child*
;
Discitis*
;
Disulfiram
;
Erythrocytes
;
Female
;
Fever
;
Gait
;
Humans
;
Leg
3.A Study of As is Patch Test in Cosmetic Contact Dermatitis.
Eun Kyoung LEE ; Jun Young LEE ; Hyung Ok KIM
Annals of Dermatology 1999;11(4):207-213
BACKGROUND: Cosmetics are one of the common causes of contact dermatitis and many new cosmetic products are being introduced rapidly into our market. OBJECTIVE: Our purpose was to investigate the as is patch test result by several factors including age, cosmetic product and atopic diseases, and to compare as is patch result with those of standard and cosmetic patch tests. METHODS: The records of 240 patients were reviewed who had been patch tested with their own cosmetics and toiletries with a clinical impression of cosmetic contact dermatitis at the patch clinic of Kangnam St. Mary's Hospital in the period of 1991-1995. RESULTS: Of the 95 patients who were tested with European standard series 54 (56.8%) showed a positive reaction and nickel sulfate was the most common allergen. Eighteen (38.3%) of the 47 patients tested with a cosmetic series revealed a positive reaction and imidazolidinyl urea and dodecyl gallate were the most common allergens. In as is patch test, 99 (41.3%) of the 240 patients showed a positive reaction to 248 (7.3%) of the 3403 cosmetics. Common cosmetic products showing positive reactions were skin care products (26.2%), face make-ups (19.8%), and hair preparations (16.9%). There was no statistically significant difference in the positive reaction rate between atopy and non-atopy patients. The positive correlation rate of as is test with standard and cosmetic series was relatively low. CONCLUSION: Commercial standard patch test series including cosmetic series are not enough to detect causative allergen in cosmetic contact dermatitis. As is patch test should be encouraged to detect new allergens, because many cosmetic ingredients are developed and introduced in market.
Allergens
;
Dermatitis, Contact*
;
Hair Preparations
;
Humans
;
Nickel
;
Patch Tests*
;
Skin Care
;
Urea
4.A Case of Sexual Precocity with Congenital Hypothyroidism.
Wan Kyu LEE ; Eun Jun HYUNG ; Duk Hi KIM
Journal of the Korean Pediatric Society 1990;33(2):259-263
No abstract available.
Congenital Hypothyroidism*
5.Postoperative Severe Hemorrhage Due to Disseminated Intravascular Coagulation: A case report.
Eun Bae CHUNG ; Seung Hee PARK ; Jun Hak LEE ; Ki Nam LEE ; Jun Il MOON
Korean Journal of Anesthesiology 1997;33(6):1220-1224
Disseminated intravascular coagulation (DIC) is a pathological syndrome in which activation of coagulation cascade leads to fibrin clot formation, consumption of platelets and coagulation factors, and secondary fibrinolysis. We report a case of severe postoperative hemorrhagic diathesis due to DIC. A 59-year-old man was scheduled for reduction of tibia fracture and anatrophic nephrolithotomy of staghorn calculi. On the fifth postoperative day, second operation was performed for nephrectomy due to perirenal hematoma. Two days later, third operation was performed for hemostasis because of the continuous bleeding. Coagulation tests showed positive DIC profiles of thrombocytopenia, hypofibrinogenemia, increased fibrin degradation products, and prolonged prothrombin time and thrombin time. The patient recovered uneventfully and discharged on the 59th postoperative day.
Blood Coagulation Factors
;
Calculi
;
Dacarbazine
;
Disseminated Intravascular Coagulation*
;
Fibrin
;
Fibrin Fibrinogen Degradation Products
;
Fibrinolysis
;
Hematoma
;
Hemorrhage*
;
Hemorrhagic Disorders
;
Hemostasis
;
Humans
;
Middle Aged
;
Nephrectomy
;
Postoperative Complications
;
Prothrombin Time
;
Thrombin Time
;
Thrombocytopenia
;
Tibia
6.Concept Analysis of the Work Interruption by Nurses
Eun Jeong YU ; Eun Nam LEE ; Jang Mi KIM ; Hey Jung JUN
Journal of Korean Academy of Nursing Administration 2019;25(4):272-281
PURPOSE: The purpose of this study was to identify the attributes, antecedents, and consequences of nurse's work interruptions. METHODS: Walker and Avant's concept analysis method was used to analyze this concept. Relevant articles published before August 2018 were searched through MEDLINE, CINAHL, EMBASE, KISS, and RISS databases using “interruption,” “work or task,” and “nurse” as keywords. RESULTS: The attributes of work interruption by nurses were as follows: 1) new tasks to do; 2) cognitive transition of work priorities; 3) loss of work continuity; 4) tasks to be resumed. The antecedents of work interruption were intrusion of unplanned events, internal and external factors that result in nurses forgetting their original intentions, an unpredictable work environment, and cultural climate where interruptions are considered as a part of the work process. The consequences of work interruption were decline in job satisfaction, trigger of work errors, lengthening of work completion time, decline in work productivity, increase in work stress, and delay of transferring needed information in a timely manner. CONCLUSION: The results of this study provide basic data to reduce the negative consequences of nurses' work interruptions, and contribute to expanding the knowledge necessary for improving patients' safety and nurses' performance.
Climate
;
Efficiency
;
Intention
;
Job Satisfaction
;
Methods
;
Walkers
;
Work Performance
7.A study on activity of purine nucleoside phosphorylase(PNP) andnatural killer(NK) cells in patients with cancer.
Tae Jun YOON ; Yung Sung LEE ; Seon Ho LEE ; Eun Yup LEE ; Soon Ho KIM
Korean Journal of Clinical Pathology 1991;11(1):153-159
No abstract available.
Humans
8.(99m)Tc-MDP Scintigraphy of Femoral Head Necrosis Following Femoral Neck Fracture
Soon Jin LEE ; Jun Hyung LEE ; Eun Kyung KIM ; Sun Wha LEE ; Soon Yong KIM
Journal of the Korean Radiological Society 1985;21(1):144-151
Secondary ischemic necrosis of femoral head due to loss of blood supply following to femoral neck fracture is well known. The regional distribution of bone.seeking radiopharmaceuricals in the skeleton can depend on a number of facto rs, but bone blood flow is a major physiological determinant of regional skeletal uptake of Tc-99m polyphosphate and bone imaging may thus be used for the evaluation of vascularity of the femoral head. The authors made a comparative study of scintigraphic findings and operative findings of 28 cases of femoral neck fracture treated at Kyung Hee University Hospital from April ′80 to May ′84. The results were as follows: 1. In 16 cases of proven avscular necrosis of femoral head, scintigraphy showed absent or decreased activity in 14 cases (87.5%), while radiography showed increased density in 10 cases (62.5%). 2. In 12 cases of proven vital femoral head, scintigraphy showed increased activity in 9 cases (75%) and radiography showed decreased density in 9 cases (75%). 3. 99mTc-MDP scintigraphy was an excellent and useful method for assessing bone vitality of femoral head.
Femoral Neck Fractures
;
Femur Head Necrosis
;
Femur Neck
;
Head
;
Methods
;
Necrosis
;
Radiography
;
Radionuclide Imaging
;
Skeleton
;
Technetium Tc 99m Medronate
9.A Case of Multiple Basal Cell Epithelioma in the Nevus Sebaceus of Jadassohn.
Seog Jun HA ; Jong Han PAIK ; Jun Young LEE ; Eun Joo SEO ; Jin Wou KIM
Annals of Dermatology 1997;9(3):208-210
Nevus sebaceus of Jadassohn is a hamartoma of the skin with the potential to develop benign and malignant neoplasms. This case was characterixed by multiple basal cell epitheliomas, clinically one reddish nodule and multiple pigmented papules, arising in the nevus sebaceus. Histologically, epithelial papillomatous hyperplasia and high-positioned hyperplastic sebaceous glands were found, and tumor nests consisting of basaloid cells with peripheral palisading arrangements were mainly situated in the upper dermis without significant infiltrative growth. We report a rare case of nevus sebaceus with multiple basal cell epitheliomas in the right cheek of a 49-year-old woman.
Carcinoma, Basal Cell*
;
Cheek
;
Dermis
;
Female
;
Hamartoma
;
Humans
;
Hyperplasia
;
Middle Aged
;
Nevus*
;
Nevus, Sebaceous of Jadassohn*
;
Sebaceous Glands
;
Skin
10.A case of Bartter's syndrome.
Jun Ho YOON ; Jeong Eun KWON ; Jae Ouk LEE ; Sang Gil LEE
Journal of the Korean Pediatric Society 1993;36(9):1308-1314
Bartter's syndrome is a rare tubular disorder characterized by hypokalemic, hypochloremic metabolic alkalosis, hyperreninemic hyperaldosteronism, hyporesponsiveness to pressor agents, and juxtaglomerular apparatus hyperplasia. We report here a case of Bartter's syndrome in a 5 month-old male infant who improved with potassium supplements. In addition to a case report, brief review of related literatures was done.
Alkalosis
;
Bartter Syndrome*
;
Humans
;
Hyperaldosteronism
;
Hyperplasia
;
Infant
;
Juxtaglomerular Apparatus
;
Male
;
Potassium