Genomic technologies have enabled rapid accumulation of information from complex biological systems over the last two decades. The complete DNA sequence is now known for many organism and the informational database obtained from genome sequencing projects has provide the base for the specification of proteome-the protein complement of genome. Genomic functions can be inferred from the analysis of gene structure and gene expression profiles because proteins are the functional molecules of an organism. Integrated technologies including protein separation, identification, characterization and information manage system are essential to analyze the proteins in complex cellular matrix. This study is focusing on the strategies of proteome analysis using sample preparation, 2-dimensional gel electrophoresis, processing of protein spots and identification of proteins, protein-protein interaction and posttranslational modification using MALDI-TOF-MS. 2-Dimensional gel electrophoresis is currently the most powerful protein separation technique and MALDI-TOF MS is powerful identification technique for protein and peptides as a sensitive, rapid, and high resolution analytical method. The developed integrated proteome technologies are very useful to understand the biological phenomena at molecular level by identifying the new molecules and their modifications in various cellular processes, and can be applied for biotechnology including medical science.
Base Sequence ; Biological Phenomena ; Biotechnology ; Complement System Proteins ; Electrophoresis ; Electrophoresis, Gel, Two-Dimensional* ; Genome ; Peptides ; Protein Processing, Post-Translational ; Proteome* ; Proteomics* ; Transcriptome

No abstract available.
Prurigo

Recently, a hydroxyapatite sphere has been increasingly used as a buried integrated orbital implant for the great improved motility of the prosthesis after enucleation. To allow more rapid ascess of vasculature into the hydroxyapatite implant, Dacron as a new synthetic wrapping material of the implant for anophthalmic sockets had been used and compared histologically with donor sclera in rabbits. At 4 weeks after implantation, fibrovascular ingrowth was found mainly near the scleral windows, where fibrosis extended 2 mm centrally in scleral-wrapped implants. In Contrast, in Dacron-wrapped implants, there was extensive fibrovascular infiltration through Dacron filaments and windows as well, making the hydroxyapatite sphere completely invested with fibrovascular tissue to the center within 4 weeks of implantation. Thus, Dacron as a new wrapping material appears to allow a more rapid vascularization into the hydroxyapatite implant than other collagen material without any serious complications, resulting in early rehabilitation with an artificial eye.
Collagen ; Durapatite* ; Eye, Artificial ; Fibrosis ; Humans ; Orbital Implants ; Polyethylene Terephthalates* ; Prostheses and Implants ; Rabbits ; Rehabilitation ; Sclera* ; Tissue Donors*

To evaluate the possibility of Dacron patch for artificial sclera. Dacron(polyethylene terephthalate) which is readily available, biocompatable, safe, and inert synthetic material was implanted in the area of full-thickness scleral defect in rabbits. The histopathologic examinations showed that fibrovascular tissue invested into all interstices of Dacron filaments and fibroconnective tissue encapsulation around Dacron patch had strong connection with collagen fibers of recipient sclera at 4 weeks after graft There were no adverse reactions such as retinal detachment and fibrous proliferation into the vitreous cavity except mild inflammatory response of surrounding tissue at the early postoperative stage. As a result, Dacron graft showed successful firm adhesion to the recipient sclera without serious complications. Thus, We think that Dacron could possibly be used, instead of donor sclera, as artificial scleral graft material for the surgical treatment of perforating scleral diseases.
Collagen ; Humans ; Polyethylene Terephthalates* ; Rabbits* ; Retinal Detachment ; Sclera ; Scleral Diseases ; Tissue Donors ; Transplants*

Candida albicans endophthalmitis following cataract surgery is fortunately rare complication, but once the infection is established, its course is unrelenting and the prognosis for a good visual result is usually very guarded. The authors experienced a case of culture-proved Candida albicans endophthalmitis following intracapsular cataract extraction in a 74-year old man. Endophthalmitis was resolved after treatment with intravitreal injection of amphotericin B, intravenous amphotericin B, oral Ketoconazole and 2% of topical Ketoconazole.
Aged ; Amphotericin B ; Candida albicans ; Candida* ; Cataract Extraction* ; Cataract* ; Endophthalmitis* ; Humans ; Intravitreal Injections ; Ketoconazole ; Prognosis

The Ministry of Health and Welfare in Korea has enforced the oral health promotion program for elderly in Korea. There are the denture delivery program and the fluoride application-scaling program for elderly. Donggu Public Health Center in Daejeon has delivered the fluoride application- scaling program for elderly since the demonstration project in 2009. The official dental hygienists had a prior consultation with Dong-gu branch of Korean Senior Citizens Association in Daejeon. We expanded the program through government office, welfare centers, and nursing homes. The participants were satisfied with the public relations (95.2%), scaling (99.7%), fluoride application (91.5%), toothbrushing education (98.6%), and denture cleaning education (96.6%). After a medial accident with persistent bleeding, the pre-inspection survey about systemic disease and medication was reinforced. The official dental hygienists have agonized over the low participation of the low-income group and the overlap benefit with health insurance benefit of scaling. We suggested it be needed the assessment of the public oral health program to overlap with health insurance benefit.
Aged* ; Dental Hygienists ; Dentures ; Education ; Felodipine ; Fluorides ; Hemorrhage ; Humans ; Insurance, Health ; Korea ; Nursing Homes ; Oral Health* ; Public Health ; Public Relations ; Toothbrushing

Korea has been running the community oral health program for the elderly, including topical fluoride application and scaling. The aim of this study was to compare the subjective and objective oral health status of 345 participants according to the number of participants in the program and of 37 participants before and after the 5-year program. The survey consisted of an interview questionnaire and oral examinations. Analysis of variance was used to compare the variables of the 345 participants according to the numbers of participants. Paired t-test was used to compare the oral health statuses before and after the 5-year program in 37 subjects. There was no difference in subjective oral health status according to the number of participants in the oral health program in the elderly, including subjective health status, subjective oral health status, satisfaction with oral health, concern about oral health, need of dental treatment, oral pain, tooth sensitivity, subjective periodontal health, and subjective symptoms of periodontitis. The community periodontal index (CPI) of the 1 time participants was significantly higher than that of 3 times, 4 times or 5 times participants in the upper center, lower left, lower center, and lower right areas. There was a significant improvement in CPI from 2.59±1.14 to 1.41±1.54 (p<0.001) and positive oral behavioral change (daily tooth brushing frequency from 2.27±0.73 to 2.54±0.90) before and 5 years after the program. However, the program did not prevent tooth loss as the numbers of the remaining teeth significantly reduced from 23.77±1.84 to 21.95±2.03 over 5 years. We showed that running the community oral health program for the elderly for more than three years might have positive effects on the periodontal health of participants.
Aged* ; Diagnosis, Oral ; Diagnostic Self Evaluation ; Fluorides ; Humans ; Korea* ; Oral Health* ; Periodontal Index ; Periodontitis ; Running ; Tooth ; Tooth Loss

OBJECTIVE: To evaluate whether 7 radiographic angles make a useful method for analysing foot deformities in children with cerebral palsy, and to assess with changes in angles after 2 years with orthoses or operations. METHOD: The talocalcaneal, talus-first metatarsal, and calcaneus-fifth metatarsal angles on the AP radiographs and the talocalcaneal, tibiotalar, talus-first metatarsal, and talohorizontal angles on the lateral radiographs were measured in 183 cerebral palsied. Seven angles were analyzed according to the clinical types, spasticity, ambulation, and age. One hundred three feet were followed up for 2 years with application of orthoses or operations. RESULTS: The frequencies of higher range in AP talocalcaneal angle were 24.4% in spastic diplegia. The increased frequencies for abnormal range increased as the grade of spasticity. Non-ambulator group had many frequencies of lower range in AP talocalcaneal angle. The age of 8~9 years showed high peak in the frequency of abnormal range. After 2 years, the frequencies of normal range were increased in groups with orthoses or operations. CONCLUSION: The radiographic angles were a useful method for observing feet of the children with cerebral palsy. If the appropriate interventions for feet were applied, the normal frequencies of radiographic angles were increased after 2 years.
Cerebral Palsy* ; Child* ; Foot Deformities* ; Foot* ; Humans ; Metatarsal Bones ; Muscle Spasticity ; Orthotic Devices ; Radiography ; Reference Values ; Walking

Purpose: This study systematically analyzed cases in South Korea wherein nurses were prosecuted for involuntary manslaughter or injury due to professional negligence in pediatric care. Methods: We analyzed the precedents using the methodology of Hall and Wright (2008) and Austin (2010). Of the 618 cases retrieved from the Supreme Court Decisions Retrieval System in South Korea, we selected the 12 cases in which children were the victims and nurses were the defendants, using a case screening methodology. Results: The most frequent penalty was a fine, and newborns were the most frequent victims. The distribution of cases according to Austin's violation categories was: improper administration of medications (n=5), failure to monitor for and report deterioration (n=4), ineffective communication (n=4), failure to delegate responsibly (n=4), failure to know and follow facility policies and procedures (n=1), and improper use of equipment (n=1). Conclusion To ensure the safety of children, nurses are required to teach and practice a high standard of care. Nursing education programs must improve nurses’ awareness of their legal obligations. Nursing organizations and leaders should also work towards enacting effective nursing laws and ensuring that nurses are aware of their legal rights and responsibilities.

PURPOSE: This research was aimed to analyze the reports of qualitative researches published in Korean Journal of Women Health Nursing (KJWHN). METHODS: Twenty qualitative researches using in-depth interviews or focus groups published in KJWHN during from 2002 to 2011 were selected for analysis. Selected reports were analyzed by consolidated criteria for reporting qualitative research (COREQ), which were a reporting guideline for qualitative researches, and consisted of thirty two items and three domains, i.e., (1) research team and reflexivity, (2) study design, and (3) data analysis and reporting. RESULTS: Personal characteristics related to credentials, gender and interviewer's bias or assumption were less reported than other items in the 1st domain. In the 2nd domain, descriptions such as a presence of non-participants, pilot test for interview guide development, and transcript return to participants were less reported than other items. In the 3rd domain, items related to number of data coder, participants checking of the finding, and clarity of minor themes were less reported than other items. CONCLUSION: It could be concluded that COREQ is a useful guideline for reporting of qualitative studies. From these results, we suggest that full items of COREQ should be considered and described when researchers report qualitative research.
Bias (Epidemiology) ; Female ; Focus Groups ; Humans ; Qualitative Research ; Reflex ; Statistics as Topic ; Women's Health