1.G Protein Oneogenes in Aeromegaly
Journal of Korean Society of Endocrinology 1994;9(3):157-162
No abstract available.
GTP-Binding Proteins
2.The effects of different hormone conditions on the structure of the thyroglobulin from cultured pig thyroid cells.
Kyung Rae KIM ; Eun Jig LEE ; Sung Kil LIM ; Hyun Chul LEE ; Kap Bum HUH
Journal of Korean Society of Endocrinology 1993;8(3):310-317
No abstract available.
Thyroglobulin*
;
Thyroid Gland*
3.Thyroglobulin synthesis in cultured porcine thyroid cells.
Kyung Rae KIM ; Eun Jig LEE ; Kyoung Mi LEE ; Sung Kil LIM ; Hyun Chul LEE ; Kap Bum HUH
Journal of Korean Society of Endocrinology 1993;8(3):303-309
No abstract available.
Thyroglobulin*
;
Thyroid Gland*
4.The Effect of Methimazole on the Thyroglobulin Synthesis in Cultured Porcine Thyroid Cells
Eun Jig LEE ; Hyun Chul LEE ; Kap Bum HUH ; Kyung Rae KIM ; Sung Kil LIM ; Kyung Mi LEE
Journal of Korean Society of Endocrinology 1994;9(4):332-336
The thioureylene drugs, propylthiouracil and methylmercaptoimidazol(MMI), exert their antithyroid effect primarily through inhibition of thyroid peroxidase-catalyzed iodination of thyroglobulin. Recently the interest about the effect to the thyroglobulin synthesis of these drugs have been increasing. So we studied the MMI effect to the thyroglobulin synthesis in cultured porcine thyroid cells. Porcine thyroid cells were isolated by sequential trypsinization in the presence of EGTA, seeded at high density(1X10^6 cells/cm^2) and cultured. One week later, MMI was added in different concentrations(0, 0.2, 1, 5mM) with TSH only or with 4H(b-TSH, Insulin, Transferrin, Hydrocortisone) or without hormone. Medias were collected after 24 hours and compared the amount of thyroglobulin secreted. And also pulse-labeling were performed with S^35 cysteine/methionine(1-2uCi/well) for 30, 60, 90min at the same conditions.There was no significant change in the amount of the secreted thyroglobulin by MMI, and there was no significant change in the pulse-labeled interacellular thyroglobulin by MMI. And also there was no significant change in the secretion of TSH-stimulated thyroglobulin by MMI. So we conclude that MMI has no effect on the thyroglobulin synthesis in cultured porcine thyroid cells and also MMI has no effect on the TSH-stimulated thyroglobulin synthesis in cultured porcine thyroid cell.
Antithyroid Agents
;
Egtazic Acid
;
Halogenation
;
Insulin
;
Methimazole
;
Propylthiouracil
;
Thyroglobulin
;
Thyroid Gland
;
Transferrin
;
Trypsin
5.The Effect of Iodine on the thyroglobulin Synthesis in Cultured Porcine Thyroid Cells
Eun Jig LEE ; Hyun Chul LEE ; Kap Bum HUH ; Kyung Rae KIM ; Sung Kil LIM ; Kyung Mi LEE
Journal of Korean Society of Endocrinology 1994;9(4):318-324
The amount of thyroglobulin synthesized from thyroid cells and stored in colloid space in very important in thyroid hormone synthesis. The thyroglobulin synthesis is mainly regulated by TSH secreted from the pituitary gland. But recently there were some reports about the possibility that iodine regulated the thyroid protein synthesis. So our studied were conducted to determined whether iodine could have inhibitory effect on thyroglobulin synthesis and methimazole could abolish the inhibitory effect of idoine.Porcine thyroid cells were isolated by sequential trypsinization in the presence of EGTA, seeded at high density(1X10^6 cells/cm^2) and cultured. One week later, Nal was added in different concentrations(10^-7, 10^-6, 10^-5, 10^-4M). 24hour medias were collected and checked the amount of thyroglobulin secreted. And also pulse-labeling were performed with[^35S] cysteine/methionine(1-2 uCi/well) for 1 hour at the same conditions. We used 3mM methimazole and 10^-4M NaI to observe the blocking effect of methimazol in iodine.The extracellular thyroglobulin secretion was significantly decreased by iodine in dose dependent manner(82.4%, 80.7%, 76.8% and 73.1% of control). And also intracellular thyroglobulin synthesis was significantly decreased by iodide in dose dependent manner(100.5%, 83.4%, 82.3% and 79.4% of control). The inhibitory effect of iodide was abolished by methimazole(74.7% to 101.3% of control). These data indicate that high iodide inhibit the thyroglobulin synthesis and secretion from the thyroid cells, and furthermore autoregulation by iodide may include thyroglobulin synthesis. And also this effect is dependent on the generation of an organic form of iodine because methimazole abolish the inhibitory effect of iodide.
Colloids
;
Egtazic Acid
;
Homeostasis
;
Iodine
;
Methimazole
;
Pituitary Gland
;
Thyroglobulin
;
Thyroid Gland
;
Trypsin
6.The effect of interleukin-1 beta on isolated rat pancreatic islets
Hyun Chul LEE ; Kwang Jin AHN ; Eun Jig LEE ; Sung Kil LIM ; Kyung Rae KIM ; Kap Bum HUH
Journal of the Korean Diabetes Association 1991;15(1):73-78
No abstract available.
Animals
;
Interleukin-1
;
Interleukin-1beta
;
Islets of Langerhans
;
Rats
8.Update in Prolactinomas.
Hanyang Medical Reviews 2012;32(4):192-196
Prolactinomas are frequent causes of gonadal dysfunction and infertility. Dopamine agonists are the main treatment of prolactinoma and the excellent efficacy of the dopamine agonist is well known. Current challenges related to the treatment of prolactinomas include the relationship of long term use of cabergoline and cardiac valvulopathy, the remission after discontinuation of dopamine agonists, the management of pregnant women with prolactinoma, and the role for surgery on long-term management of prolactioma. Herein, these current issues and questions in the management of prolactinomas, including safety of cabergoline, recurrence after discontinuance of dopamine agonist, pregnancy, and role of surgical resection are addressed.
Dopamine Agonists
;
Ergolines
;
Female
;
Gonads
;
Heart Valve Diseases
;
Humans
;
Infertility
;
Pregnancy
;
Pregnant Women
;
Prolactinoma
;
Recurrence
9.Recent review on medical treatment of thyroid disease.
Journal of the Korean Medical Association 2012;55(12):1207-1214
Thyroid disorder is a common disease. Graves' disease is the most frequent cause of thyrotoxicosis and pharmacological treatment is current trends worldwide. Because of the severe adverse effects of propylthiouracil, methimazole or carbimazole should be selected as the drug of choice except for special situations such as women in the first trimester of pregnancy, thyroid storm, or in patients with severe side effects to methimazole. Treatment should continue for 12 to 18 months, but duration can be adjusted depending on the patient. For hypothyroidism, synthetic levothyroxine is the mainstay of treatment. In order to avoid overtreatment, the dosage of levothyroxine should be determined in consideration of the patient's age, sex, bodyweight, general condition, and comorbidities. In subclinical hypothyroidism, thyroid hormone replacement is suggested in patients with thyroid stimulating hormone concentrations >10 mIU/L. For non-elderly patients with high titers of thyroid autoantibodies, patients with dyslipidemia, pregnant patients, and women with infertility or ovulatory dysfunction, treatment with levothyroxine can be considered.
Antithyroid Agents
;
Autoantibodies
;
Carbimazole
;
Comorbidity
;
Dyslipidemias
;
Female
;
Graves Disease
;
Humans
;
Hyperthyroidism
;
Hypothyroidism
;
Infertility
;
Methimazole
;
Pregnancy
;
Pregnancy Trimester, First
;
Propylthiouracil
;
Thyroid Crisis
;
Thyroid Diseases
;
Thyroid Gland
;
Thyroiditis
;
Thyrotoxicosis
;
Thyrotropin
;
Thyroxine
10.Endocrine Regeneration Therapy using Adenoviral vector.
Journal of Korean Society of Endocrinology 2005;20(4):301-305
No Abstract available.
Regeneration*