OBJECTIVES: Cadherin/catenin adhesion complex is fundamentally involved in epithelial cancer invasion and metastasis. E-cadherin and EGFR colocalize on the basolateral membrane of epithelial cell and EGF down-regulate E-cadherin expression. In the invasion and metastasis of cancer, E-cadherin expression is decreased and growth factors receptor is overexpressed. The present study was aimed to find the role of E-cadherin, beta-and gamma-catenin, growth factors and its receptors in cervical cancer cell lines. METHODS: The cervical cancer cell cultures were treated with different time duration of EGF 30 ng/ml and TGF-a 10 ng/ml(0, 10 min, 20 min, 30 min, 1 hr, 2 hr, 4 hr, 8 hr, 24 hr). The change in cancer cell morphology and the changes in E-cadherin, beta- and gamma-catenin, EGFR and activated EGFR expression were studied with a western blot analysis and an immunoprecipitation. RESULTS: Through a western blot analysis, E-cadherin 120 kDa band and EGFR 170 kDa band were expressed in CaSki, HT-3 and ME-180 cell line, which showed epithelial contact growth. 1n these 3 cell lines, expression of E-cadherin did not decrease with time dependent manner. after the treatment of EGF and TGF- alpha. The expression of EGFR decreased and activated EGFR expression increased in 30 minutes to 1 hour but decreased subsequently. When the cells treated with EGF, there were no change in beta-and gamma-catenin expression with there dependent manner. The tyrosine phosphorylation of beta-and gamma-catenin increased in 30 minutes to 1 hour but decreased subsequently with activated EGFR. CONCLUSION: This study showed that an activated EGFR which has involved with tyrosine phosphorylation of beta- and gamma-catenin influenced by growth factors rather than expression of E-cadherin, has a role in the invasion and metastasis of the cervical cancer.
Blotting, Western ; Cadherins* ; Cell Culture Techniques ; Cell Line* ; Epidermal Growth Factor* ; Epithelial Cells ; gamma Catenin* ; Immunoprecipitation ; Intercellular Signaling Peptides and Proteins ; Membranes ; Neoplasm Metastasis ; Phosphorylation* ; Transforming Growth Factor alpha* ; Tyrosine* ; Uterine Cervical Neoplasms*

Autosomal dominant polycystic kidney disease (ADPKD) is a hereditary disorder characterized by renal cyst formation, hypertension, and end-stage renal disease. For many years, ADPKD was considered an adult disease. In fact, it may occur at any time in life including in utero. We experienced a case of ADPKD at 34 weeks of gestation. On ultrasound, both kidnies were enlarged and echogenic. Amniotic fluid index was normal. We discovered a family history of paternal origin. Sonographic findings of bilaterally enlarged and echogenic kidnies without oligohydroamniosis may suggest ADPKD. Renal ultrasound examination of parents is useful in the diagnosis of ADPKD.
Adult ; Amniotic Fluid ; Diagnosis* ; Female ; Humans ; Hypertension ; Kidney Failure, Chronic ; Parents ; Polycystic Kidney, Autosomal Dominant* ; Pregnancy ; Prenatal Diagnosis ; Ultrasonography

No abstract available.
Antioxidants* ; Oxidative Stress*

Pulmonary angiodysplasia indicates all forms of abnormal intrapulmonary arteriovenous comunication, usuallycalled pulmonary arteriovenous fistula. Most frequent form of pulmonary angiodysplasia is single or multiplediscrete nodular lesion, and diffuse telangiectatic type consists another variety. More than 50% of pulmonaryangiodysplasias are associated with hereditary hemorrhagic telangiectasia. And rearely chronic liver disease iscomplicated by pulmonary arteriovenous fistula which is called hepatogenic pulmonary angiodysplasia. Weexperienced 6 cases of pulmonary angiodysplasia confirmed by cardiac catheterization and angiography from 1977 to1983. 5 cases were children below 11 years of age exhibiting cyanosis except one who has pulmonary angiodysplasiasupplied by systemic arteries. 3 were hereditary hemorrhagic telangiectasia and one was hepatogenic pulmonaryangiodysplasia. Discrete nodular pulmonary lesion was present in 3 cases while remaining 3 cases were diffusetelangiectatic type. The clinical and radiological findings of pulmonary angiocysplasia is described with a briefreview of literature.
Angiodysplasia ; Angiography ; Arteries ; Arteriovenous Fistula ; Cardiac Catheterization ; Cardiac Catheters ; Child ; Cyanosis ; Humans ; Liver Diseases ; Telangiectasia, Hereditary Hemorrhagic

Radiation exposure dose for patients during brain CT scan with General Electric C.T./T.8800 was measured using CaF. :Mn thermoluminescent dosimeter chips. The skin dose in single slice scan was 1.26±0.1 0 rad, and that in 9 slice scan was 2.06±0.16 rad. So the ratio was 1.63. The surface dose in profile along axial distance in multiple slice scan was also measured, and was found to be low at the junction of 2 slices. The lens dose in multiple slice scan was 0.27±0.05 rad , too small to induce any pathologic condition. As noted from the above data, the radiation exposure dose during CT scanning is not high compared with other radiologic examinations.
Brain ; Humans ; Radiation Exposure ; Skin ; Tomography, X-Ray Computed

BACKGROUND: This study researched the relationship between shift work and one of its relevant health problems, functional gastrointestinal disorders. The study targeted nurses working at a university hospital, and compared the differences in gastrointestinal disorders of shift workers and non-shift workers and identified causes that significantly influence these symptoms. METHODS: In August 2013, a self-report survey was conducted on nurses working at a university hospital. Among those sent out, 1,201 responded and 851 were used for the final analysis. RESULTS: It was shown that the frequency of gastrointestinal disorders in shift workers was 1.881 times higher than that of non-shift workers. Factors that influenced gastrointestinal disorders included age, marital status, work duration, type of employment, job satisfaction, and perceived health status. Gastrointestinal disorders were seen more often with older age, being married, longer duration of employment, non-regular worker, low job satisfaction, bad perceived health status, and shift works. CONCLUSIONS: To prevent gastrointestinal disorders in nurses who work shifts, a health checkup system should be in place for clinical nurses who are of older age and have longer employment records. To decrease the causes of gastrointestinal disorders, campaigns suggesting regular diet and quitting alcohol use should be held consistently. Also, a category related to gastrointestinal disorders should be added to the employee health checkup for nurses with long work experience.
Diet ; Employment ; Gastrointestinal Diseases ; Job Satisfaction ; Marital Status ; Occupational Health

A 52-year-old woman was admitted with a palpable mass on the abdominal wall. This tumor had developed at the same site of a previously resected dermatofibrosarcoma protuberans. The biopsy specimen from the recurrent tumor revealed malignant fibrous histiocytoma. Immunohistochemistry with anti-CD34 antibody revealed diffuse linear positivity on the tumor cells of dermatofibrosarcoma protuberans and negativity on malignant fibrous histiocytoma. Anti CD34 antibody was valuable for a differential diagnosis of these two lesions.
Abdominal Wall ; Biopsy ; Dermatofibrosarcoma* ; Diagnosis, Differential ; Female ; Histiocytoma, Malignant Fibrous* ; Humans ; Immunohistochemistry ; Middle Aged

This study was performed for the comparison of the therapeutic efficiency between 6-month (2tHER/4HER) and 9-month (9HER) short-course chemotherapy under the programe conditions for pulmonary tuberculosis in terms of sputum AFB negative conversion rate, remedial interruption rate and cost effectiveness analysis. Two hundreds and ninty three patients treated with 9HER and 641 treated with 2HERZ/4HER had been discharged from 22 health centers in Seoul from May 1, 1993 to April 30, 1994. Seven hundreds and seventeen was subsequently analysed excluding 217 patients due to remedial interruption. The results: 1. Bacteriological negative conversion rate in 9HER regimen and 2HERZ/4HER regimen was 97.8% and 96.4% respectively (p>0.05). But the early treatment period, negative conversion rate in 2HERZ/4HER regimen was very higher than in 9HER regimen(p<0.01). 2. Remedial interruption rate for 9HER regimen and 2HERZ/4HER regimen was 34.1% and 13.6% respectively. The primary reason for the interruption was transfering to other clinics and this interruption was high within 3months. 3. Cost effectiveness for 2HERZ/4HER regimen was higher than 9HER regimen. The difference cost effectiveness ratio was 2.33 at the first sputum test and 1.69 at the last sputum test.
Cost-Benefit Analysis ; Drug Therapy* ; Humans ; Seoul* ; Sputum ; Tuberculosis, Pulmonary*

No abstract available.

PURPOSE: The purpose of this study was to identify relationships among self-esteem, social support, nursing organizational culture, experience of workplace bullying, and the consequences of workplace bullying in hospital nurses, and then to provide basic information for developing workplace bullying prevention programs. METHODS: Participants were 122 hospital nurses from three general hospitals. Data collection was done during April and May 2015. Data were collected using self-report questionnaires which were used to identify participants' characteristics, self-esteem, social support, nursing organizational culture, and workplace bullying. RESULTS: Approximately one quarter of the nurses had experienced workplace bullying in the past six months. Hierarchical regression analysis showed that experience of workplace bullying (β=.45) and self-esteem (β=-.31) explained 53.3% of the variance in consequences of workplace bullying. CONCLUSION: Based on the findings that experiencing workplace bullying and having a low self-esteem were likely to increase workplace bullying in hospital nurses, there is a need to develop prevention and intervention programs on avoiding or dealing with workplace bullying.
Bullying* ; Data Collection ; Hospitals, General ; Nursing* ; Organizational Culture*