1.Expression of bcl-2 and p53 Protein in Invasive Cervical Cancer.
Cheon Jun LEE ; Eun Mo AHN ; Tae Hong YEO ; Dong Hwi KIM ; Un Dong PARK
Korean Journal of Gynecologic Oncology and Colposcopy 1999;10(3):272-279
Recently, the bcl-2 and p53 protein have been recognized as important factors that is contributed to programmed cell death. The objective of this study was to evaluate the prognostic significance of bcl-2 and p53 protein expression in uterine cervical carcinoma. The expression of bcl-2 and p53 in 59 cases of uterine cervical carcinoma (stage IB to IIB) were surgically treated from January 1993 to June 1994. The expression of bcl-2 and p53 was examined by immunohistochemical method using formalin fixed paraffin embedded tissue specimens. The 48 cases were squamous cell carcinoma and 11 cases were adenocarcinoma. The results were as follows: 1. The expression rate of bcl-2 protein was 28.8%(17/59) and there was no significant correlaltion between the expression of bcl-2 protein and the clinicopathologic parameters (histologic type, grade, FIGO stage, cervical invasion depth, lymph node metastasis, parametrial invasion, tumor size, neoadjuvant chemotherapy response, recurrence, survival). 2. The expression rate of p53 protein was 32.2%(19/59) and there was no significant correlation between expression of p53 protein and the clinicopathologic parameters. 3. There was significant correlation between and expression of bcl-2 and p53 protein (P 0.05). In conclusion, bcl-2 and p53 protein are thought to be possible factors in the carcinogenesis of uterine cervical carcinoma and correlate with progression of it. But further study will be required to clarify the role of bcl-2 and p53 in carcinogenesis of the uterine cervix.
Adenocarcinoma
;
Carcinogenesis
;
Carcinoma, Squamous Cell
;
Cell Death
;
Cervix Uteri
;
Drug Therapy
;
Female
;
Formaldehyde
;
Lymph Nodes
;
Neoplasm Metastasis
;
Paraffin
;
Recurrence
;
Uterine Cervical Neoplasms*
2.Diagnostic Conization of the Cerivx.
Gun Sang YOO ; Dong Hwi KIM ; Eun Hee CHYU ; Un Dong PARK
Korean Journal of Gynecologic Oncology and Colposcopy 1996;7(2):77-83
To evaluate the effectiveness of conization in the diagnosis of cervical cancer, the histopathologic finding of 464 consecutive cases were analized with respect to the cytology, punch biopsy and hysterectomy. Cold knife conization was performed under direct visualization(naked eye conization) from January, 1987 to Octorber 1994 at department of Obstetrics and Gynecology, Kosin Medical Center. The results were summerized as follows : 1. The rate of agreement and underdiagnosis of between pap smear and naked eye conization were 43.7% and 47%. 2. The rate of agreement and underdiagnosis of between punch biopsy and naked eye conization were 64.5% and 21.8% 3. Subsequent hysterectomy was done on the 382 case and their incidence of residual disease rate was 44.2%(169 cases). Their incidence of residual lesion was increased with severity of the carcinomatous change. 4. The rate of complication undergoing conization was 8.4%(39 cases).
Biopsy
;
Conization*
;
Diagnosis
;
Gynecology
;
Hysterectomy
;
Incidence
;
Obstetrics
;
Uterine Cervical Neoplasms
3.Current Immune Status of Children and Pregnant Women in Korea(I).
Eun Jung KIM ; Ju Kwan PARK ; Chang Hwi KIM ; Sang Jhoo LEE
Journal of the Korean Pediatric Society 1989;32(5):620-629
No abstract available.
Child*
;
Female
;
Humans
;
Pregnant Women*
4.The clinical value of the ultrasonography in the diagnosis of the malignant ovarian ca.
Kwang Hwi PARK ; Sun Kyung LEE ; Byung Hee SUH ; Jae Hyun LEE ; Jung Eun MOK
Korean Journal of Obstetrics and Gynecology 1993;36(4):543-552
No abstract available.
Diagnosis*
;
Ultrasonography*
5.The clinical value of the ultrasonography in the diagnosis of the malignant ovarian ca.
Kwang Hwi PARK ; Sun Kyung LEE ; Byung Hee SUH ; Jae Hyun LEE ; Jung Eun MOK
Korean Journal of Obstetrics and Gynecology 1993;36(4):543-552
No abstract available.
Diagnosis*
;
Ultrasonography*
6.Three Cases of Kawasaki Disease Accompanied with Rare Clinical Menifestation.
Min Yong OUM ; Sang Eun LEE ; Jae Ock PARK ; Chang Hwi KIM ; Sang Jhoo LEE
Journal of the Korean Pediatric Society 1989;32(9):1300-1308
No abstract available.
Mucocutaneous Lymph Node Syndrome*
7.Follow up Study of Second Look Laparotomy in Ovarian Cancer patients.
Jun Mo AN ; Dong Hwi KIM ; Hyun KIM ; Gun Sang YOO ; Choi In SEOK ; Eun Hee JOO ; Un Dong PARK
Korean Journal of Gynecologic Oncology and Colposcopy 1996;7(2):93-99
A study was to evaluate the efficacy and clinical utility of second look operation in the managements of malignant ovarian tumor at the Department of Obstetrics and Gynecology, Kosin medical colleage from January 1986 to September 1993. Of 220 patients diagnosed as ovarian cancer, 48 patients who had underwent primariy laparotomy were performed second look operation. Thirty-seven patients were found to have no evidence of disease. The result revealed that negative second look operation rate was noted 96.3% at stage I, 66.6% at stage II, 50.0% at stage III, and 42.9% at stage IV and total negative second look operation rate was 77.1%. The 2 year survival rate for stage I, II, III, IV were 85.2%, 66.6%, 50.0% and 42.9% respectively. The survival rate of negative second look operation was 86.5%, that of positive second look operation was 18.1%. In conclusion, second look operation in early stage ovarian cancer need to be justified.
Follow-Up Studies*
;
Gynecology
;
Humans
;
Laparotomy*
;
Obstetrics
;
Ovarian Neoplasms*
;
Survival Rate
8.Influence of Estrogen and Polyamines on Mifepristone-induced Apoptosis in Prostate Cancer Cells.
Eun Kyung CHOI ; Hwi June SONG ; Min S PARK ; Byeong Gee KIM
Cancer Research and Treatment 2004;36(1):85-90
PURPOSE: Although androgens are the main steroids controlling the growth of prostate glands, estrogens are also important in the regulation of its growth. Prostate cancer cells, like other cancer cells, maintain high levels of polyamines. In LNCaP cells, apoptosis is induced by mifepristone. During the process of cell death, the regulation of ROS production, caspase-3 activation and poly (ADP-ribose) polymerase cleavage were investigated in the presence of estrogen and polyamines to identify their possible roles. MATERIALS AND METHODS: The cell growth was assessed using the MTT assay, and the intracellular ROS production by the DCFH-DA assay. The p53 protein expression, activation of caspase-3 and PARP cleavage were checked by Western blotting, with specific antibodies to each. RESULTS: The growth and viability of the cells were significantly inhibited, in a dose- and time-dependent manners, by mifepristone (MIF) treatment. The production of ROS were dependent on the MIF dosage. The activation of caspase-3 and cleavage of PARP also increased with the duration of MIF treatment. The expression of p53 protein also increased with increases in the MIF incubation time. E2 severely inhibited the ROS production, caspase-3 activation and PARP cleavage. However, polyamines only inhibited the ROS production, without influencing the caspase-3 activation or PARP cleavage. CONCLUSION: In LNCaP cells, MIF induces apoptosis through ROS production. The expression of p53 protein, caspase-3 activation and PARP cleavage accompanied the process of apoptosis. The apoptotic processes were inhibited by E2, but polyamines only inhibited the ROS production, implying the multifunctional role of E2, in addition to its role as a free radical scavenger.
Androgens
;
Antibodies
;
Apoptosis*
;
Blotting, Western
;
Caspase 3
;
Cell Death
;
Estrogens*
;
Mifepristone
;
Polyamines*
;
Prostate*
;
Prostatic Neoplasms*
;
Steroids
9.A clinico - Pathological study on malignant ovarian tumors.
Rhi Ae JU ; Kwang Hwi PARK ; Seon Kyung LEE ; Bo Hoon OH ; Jae Hyun LEE ; Jung Eun MOK
Korean Journal of Obstetrics and Gynecology 1993;36(7):1434-1443
No abstract available.
10.A case of hepatoma with hot uptake of Tc-DISIDA to spleen.
Ye Bong LEE ; Sung Jin KIM ; Eun Hwi PARK ; In Sook BANG ; Heung Joo KIM ; Sung Chul YUN ; Won Jae LEE
Korean Journal of Nuclear Medicine 1993;27(1):140-145
No abstract available.
Carcinoma, Hepatocellular*
;
Spleen*