No abstract available.
Stomach Neoplasms* ; Weight Loss*

No abstract available.
Adenocarcinoma* ; Epidermal Growth Factor* ; Recurrence*

OBJECTIVES: To determine the level of mRNA expression of various members of the matrix metalloproteinase and tissue inhibitors in uterine leiomyoma compared with unaffected myometrium. Materials & Method: 30 cases of portions of leiomyoma and myometrium were collected immediately followimg hysterectomy. Thirteen cases were from proliferative phase and seventeen were from secretory phase of menstrual cycle. The mean age was 43.7years old. The level of expression of mRNAs of interstitial collagenase, gelatinase, stromelysin, TIMP-1,-2,-3 was determined by reverse transcriptase-polymerase chain reaction(RT-PCR) and normalized to GAPDH(glyceraldehyde-3-phosphate dehydrogenase) mRNA. RESULTS: Myometrium and leiomyoma expressed all the members of above mentioned matrix metalloproteinase family and tissue inhibitors. Leiomyoma expressed a significantly higher level of stromelysin-3 during secretory phase, an extremely lower level of 92kDa gelatinase and a significantly lower level of TIMP-3. The immunohistochemical localization of TIMP-3 was smooth muscle cell and arteriole wall of myometrium and leiomyoma. CONCLUSIONS: The increased expression of stromelysin-3 in uterine leiomyoma compared with myometrium suggests that this MMP may be involved in the formation of a more fibrous extracellular matrix in leiomyoma. The extremely lower expression of 92kDa gelatinase of leiomyoma means that leiomyoma do not invade myometrium and forms a separated mass. Decreased expression of TIMP-3 of leiomyoma suggests that TIMP-3 is required for differentiation and homeostasis of extracellular matrix of normal myometrium and function as a suppressive role of tumor development
Animals ; Arterioles ; Extracellular Matrix ; Female ; Gelatinases ; Homeostasis ; Humans ; Hysterectomy ; Leiomyoma* ; Matrix Metalloproteinase 1 ; Matrix Metalloproteinase 3 ; Menstrual Cycle ; Mice ; Myocytes, Smooth Muscle ; Myometrium* ; RNA, Messenger ; Tissue Inhibitor of Metalloproteinase-3

Prurigo pigmentosa is a chronic pruritic inflammatory dermaoss characterized by erythematous papules in a reticulated pattern that resolve leaving a reticulated, mottled hyperpigmentation. Most cases have been reported from Japan. Two cases of prurigo pigmentosa have been reported in Korean women. We experienced a casc of prurigo pigmentosa in a Korean man of 20 years of age. Histopathological findings of reddish papule showed exocytosis, sponsiosis, intraepidermal vesicles, hydropic degeneration of basal cells in the epidermis, and hyphohistiocytic infiltration in the upper dermis. Direct irnmunofluorescence was negetive. Therapy with dapsone, 50 mg given daily, resulted in a rerinable regression of the reddish papules. The daily dose of clapsone was reduced to 25mg; however, no new reddish, pruritic papules have appeared.
Dapsone ; Dermis ; Epidermis ; Exocytosis ; Female ; Humans ; Hyperpigmentation ; Japan ; Prurigo*

The clinical use of topical antimicrobial substances is common in clean wounds as well as contaminated and chronic wounds. This use is based on the observation that the reduction of superficial bacterial contamination promotes wound healing. While the antibacterial efficacy of topical antimicrobials has been well documented, their effects on living tissue and the process of wound healing remain controversial. Recently, there have been several trials to detect the toxicity of various antimicrobial materials by using cultured fibroblasts and keratinocytes. In this study the cytotoxicity of several commonly used antimicrobials and the cytotoxic concentrations of antimicrobials on cultured human keratinocytes and fibroblasts were compared using 3 different METHODS: 1) the MTT method showing the ability of the cells to clear the cytotoxic agents, 2) LDH release indicating damage of cell membrane, and 3) tritiated thymidine incorporation measuring DNA synthesis. In the MTT method all topical antimicrobials in their usual clinical concentration showed cytotoxicity on both keratinocytes and fibroblasts by suppressing cell proliferation. Keratinocytes were more sensitive to certain antibiotics. When the 50% inhibition concentration (IC50) of topical antimicrobials were compared, the cytotoxicity to keratinocyte was, in the decreasing order, chlorhexidine, hydrogen peroxide, povidone iodine, sulfadiazine, fusidic acid, ethanol. Gentamicin and bacitracin. The cytotoxicity to fibroblasts was, in decreasing order, chiorhexidine, hydrogen peroxide, povidone iodine, fusidic acid, ethanol, sulfadiazine, bacitracin, and gentamicin. Most of the antimicrobials tested increased LDH release by keratinocyte in IC50.Chlorhexidine, hydrogen peroxide and povidone iodine showed statistically significant increase in LDH release. The tritiated thymidine incorporation study showed the order of cytotoxicity to keratinocyte in IC50 of each antimicrobials was similar to that of the MTT method. Tritiated thymidined incorporation, however, appeared to be a more sensitive method in detecting cytotoxicity than MTT because the IC50 was lower in the former. In conclusion, all topical antimicrobials are cytotoxic to keratinocytes and fibroblast in their usual concentration. Because of their cytotoxicity it is suggested that one should use topical antimicrobials in a dilution of up to 1 : 1,000 of commonly used concentration for a short period only especially when applied directly on a skin graft or wounds without a protective barrier. Bacitracin or gentamicin appear to be less cytotoxic among all the antimicrobials tested.
Anti-Bacterial Agents ; Bacitracin ; Cell Membrane ; Cell Proliferation ; Chlorhexidine ; Cytotoxins ; DNA ; Ethanol ; Fibroblasts* ; Fusidic Acid ; Gentamicins ; Humans* ; Hydrogen Peroxide ; Inhibitory Concentration 50 ; Keratinocytes* ; Povidone-Iodine ; Skin ; Sulfadiazine ; Thymidine ; Transplants ; Wound Healing ; Wounds and Injuries

BACKGROUND: Treatments inhibiting Propionibacterum acnes as like antibiotics for acne vulgaris can produce complications, and have their limited uses.OBJECTIVE: Antibodies reacting to P. acnes were produced from immunized chicken eggs, and were characterized by aggulutination test and inhibiting abilities to growth of P. acnes. METHODS: P. acnes were cultured, and their lipase were used as immunogen. Antibodies were purified from immunized chicken eggs by diafiltration. Their titers were measured by ELISA. Antigen-antibodies agglutination test was performed, and the ability of inhibitions of P. acnes grown in media was observed. RESULTS: Antibodies were well produced especially when 0.25 to 2 mg of immunogen were injected per one chicken. When antibodies were reacting to P. acnes, pellet formations were not shown because of agglutination above 17.8 pg concentration of antibodies, and acitivities of P. acnes were markedly suppressed on microscopic examination. Growth of P. acnes on media were also inhibited for quite a long time. CONCLUSION: Antibodies reacting to P. acnes were produced, and their abilities to inhibit the growth of P. acnes were confirmed. In future, produced antibodies from immunized chicken eggs would be hoped to be a main component of medication or cosmetics for improving or preventing acne lesions.
Acne Vulgaris ; Agglutination ; Agglutination Tests ; Anti-Bacterial Agents ; Antibodies* ; Chickens* ; Eggs* ; Enzyme-Linked Immunosorbent Assay ; Hope ; Lipase ; Ovum* ; Propionibacterium acnes* ; Propionibacterium*

No abstract available.
Atrophy* ; Magnetic Resonance Imaging* ; Myelitis, Transverse* ; Spinal Cord*

Epithelioid hemangioendothelioma(EH) is a low-grade tumor of vascular endothelial cell origin, which most commonly occur in the lower extremities of middle-aged patients with an equal gender distribution. It may occur on internal organs such as the liver or lung or bones. A 54-year-old woman who had a history of metastatic adenocarcinoma of unknown origin on the right inguinal lymph node, was presented to us because of a 1.0 x 1.0cm-sized and 2 x 2cm-sized two subcutaneous nodule on her right shin. On H & E stain, there were nests of round nucleated, polygonal, vacuolated cells, some of which contained fragmented erythrocytes. Factor VIII related antigen, CD31 was positive. A diagnosis of epithelioid hemangioendothelioma was established based on histologic, immunohistochemical studies. Wide excision was performed. There was no recurrence nor the development of new lesions so far.
Adenocarcinoma ; Diagnosis ; Endothelial Cells ; Erythrocytes ; Female ; Hemangioendothelioma, Epithelioid* ; Humans ; Liver ; Lower Extremity ; Lung ; Lymph Nodes ; Middle Aged ; Recurrence ; von Willebrand Factor

No abstract available.
Spinal Cord Compression* ; Spinal Cord* ; Thymoma*

Bladder dysfunction is a common occurrence following radical hysterectomy. It is a direct consequence of the intraoperative disruption of the autonomic nerve supply to the bladder. We herein evaluated 158 patients who underwent radical hysterectomy for carcinoma of cervix in the Dept. of Obstet.-Gynecol., Seoul National University Hospital into two separate part. In Part 1,140 patients who received the surgery from Jan. 1980 to Dec 1981 were subjected to this study. The records and the follow-up results were analysed retrospectively. Immediate postoperatively, 44.7% of patients presented bladder dysfunction such as large residual urine over 100 cc and delayed perception of bladder filling sensation. In 6 month follow-up group, 15.8% of patients complained urologic symptoms such as strain. stress incontinence, urgency and sensory loss. In 1 year follow-up group, the same symptoms were persisted in 11.5% of patients. In Part 2, urodynamic test was performed in 10 patients preoperatively and 18 patients postoperatively. No differences were found between two groups except marked increase of bladder volume at the first voiding sensation. Urecholine denervation test of Lapides was done in 18 patients postoperatively and three patients revealed positive test, which means partial parasympathetic detrusor denervation. To decrease the incidence of bladder dysfunction, the use of suprapubic cystostomy, subcutaneous injection of urecholine, intermittent self catheterization are recommended rather than indwelling urethral catheter. Urodynamic test is necessary to define the nature and to manage the patients with bladder dysfunction after radical hysterectomy.
Autonomic Pathways ; Bethanechol Compounds ; Catheterization ; Catheters ; Cervix Uteri ; Cystostomy ; Denervation ; Female ; Follow-Up Studies ; Humans ; Hysterectomy* ; Incidence ; Injections, Subcutaneous ; Retrospective Studies ; Sensation ; Seoul ; Urinary Bladder* ; Urinary Catheters ; Urodynamics