Objective: To investigate the parasitic infection status of cyprinid fish, Capoeta damascina in Gomban spring-stream, Kor River Basin, Fars Province, southwestern Iran.Methods:stream, Kor River Basin, Fars Province, southwestern Iran. The collected fish were dissected carefully and their internal organs such as liver, gonad, muscle, abdominal lobes, whole viscera and digestive tract were surveyed parasitologically.Results:One female cyprinid fish out of 12 fish was infected with three nematodes. Two A total of 12 cyprinid fish (7 females and 5 males) were collected from Gomban spring-nematodes (larvae) were identified as Contracaecum sp. which were attached firmly to the outer part of intestine and another adult helminth was recognized as Capillaria sp. which was recovered from digestive content.Conclusions:This study is the first record indicating that cyprinid fish acts as a new host for recovered nematodes. Further helminthological investigations are highly recommended in different parts of Iran in order to expand our knowledge about helmintic parasites of cyprinid fish and their role in transmission of diseases to human and animal.

The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0–1.8% and 0–0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.
Animals ; Base Sequence ; Cattle ; Databases, Nucleic Acid ; DNA ; France ; Goats ; Humans* ; Iran* ; Laos ; Methods ; New Zealand ; Polymerase Chain Reaction ; Sheep ; Thailand ; Trichostrongylus*

Strongyloides stercoralis is a human intestinal parasite which may lead to complicated strongyloidiasis in immunocompromised. Here, a case of complicated strongyloidiasis in a patient with chronic lymphocytic leukemia is reported. Presence of numerous S. stercoralis larvae in feces and sputum confirmed the diagnosis of hyperinfection syndrome in this patient. Following recovery of filariform larvae from agar plate culture of the stool, the isolate was characterized for the ITS1 region of ribosomal DNA gene by nested-PCR and sequencing. Albendazole therapy did not have cure effects; and just at the beginning of taking ivermectin, the patient died. The most important clue to prevent such fatal consequences is early diagnosis and proper treatment.
Aged ; Albendazole/therapeutic use ; Animals ; Anthelmintics/therapeutic use ; Fatal Outcome ; Humans ; Ivermectin/therapeutic use ; Larva ; Leukemia, Lymphocytic, Chronic, B-Cell/*complications/parasitology ; Male ; Strongyloides stercoralis/*classification ; Strongyloidiasis/*complications/drug therapy

OBJECTIVETo investigate the parasitic infection status of cyprinid fish, Capoeta damascina in Gomban spring-stream, Kor River Basin, Fars Province, southwestern Iran.

METHODSA total of 12 cyprinid fish (7 females and 5 males) were collected from Gomban spring-stream, Kor River Basin, Fars Province, southwestern Iran. The collected fish were dissected carefully and their internal organs such as liver, gonad, muscle, abdominal lobes, whole viscera and digestive tract were surveyed parasitologically.

RESULTSOne female cyprinid fish out of 12 fish was infected with three nematodes. Two nematodes (larvae) were identified as Contracaecum sp. which were attached firmly to the outer part of intestine and another adult helminth was recognized as Capillaria sp. which was recovered from digestive content.

CONCLUSIONSThis study is the first record indicating that cyprinid fish acts as a new host for recovered nematodes. Further helminthological investigations are highly recommended in different parts of Iran in order to expand our knowledge about helmintic parasites of cyprinid fish and their role in transmission of diseases to human and animal.