1.Cytological study of macrophage migration inhibitory factor in treatment of steriod-induced ischemic osteonecrosis of femoral head
Jun MA ; Eryang ZHANG ; Wenrui BAN ; Chen ZHANG ; Xiaoqian DANG ; Kunzheng WANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2017;38(2):182-187
Objective To explore whether macrophage migration inhibitor factor (MIF)can protect human bone microvascular endothelial cells (HBMECs)from glucocorticoid-induced damage.Methods HUVECs were isolated from human femoral head.After HUVECs were cultured and identified,we constructed the ECs damage model with high-dose hydrocortisone.The cells were randomly divided into blank control group,low-dose MIF group,high-dose MIF group with corresponding treatment.Cell activity was detected by AlamarBlue in each group. The number of viable cells was detected in Live/Dead staining.The cell morphology was observed after cytoskeleton staining.Cell migration ability was compared by scratch test and the level of VEGF expression was detected by ELISA.Results Cell model was successfully constructed.The activity of cells in high-dose MIF group (178.3± 15.2)% was significantly higher than that in the control group (100±8.4)% and low-dose MIF group (149.1± 13.8)% (P<0.05).The number of viable cells in high-dose MIF group (139.5±14.3)% was higher than that in low-dose MIF group (121.3±12.9)% while the two groups had more viable cells than the control group (100± 8.4)% (P<0.05).The scratch test results indicated that cell migration ability in high-dose group was the strongest and the scratch disappeared at 24 hours after scratching.The expression of VEGF at 24 hours after intervention was (170±15.7)pg/mL in normal group,(328±25.3)pg/mL in low-dose group and (405±31.2)pg/mL in high-dose group.VEGF level was lower in low-dose group than in high-dose group (P<0.05),but higher than the normal group (P<0.01).Conclusion MIF can promote the proliferation and migration of ECs in a dose-dependent manner and upregulate the expression of VEGF.MIF can improve ECs damage induced by high-dose glucocorticoid.
2.Survey on the Cognition of Medical Staff to Clinical Pharmacy Work in Primary Hospitals
China Pharmacist 2018;21(6):1041-1043
Objective: To investigate the cognition of medical staffs in grass-roots hospitals to clinical pharmacy practice and clini-cal pharmacists to provide some references for the further development of clinical pharmacy service. Methods: Totally 625 clinicians and nurses from 4 grass-roots hospitals were surveyed using a questionnaires, and the results were statistically analyzed. Results: The clinicians and nurses had an approval attitude towards clinical pharmacy practice and clinical pharmacists with small difference (overall average score was 3. 73±0. 86 and 3. 70±0. 90, respectively). They showed the highest cognition to clinical pharmacy practice, and the lowest cognition to the development, theory and regulation of clinical pharmacy and clinical pharmacists. Conclusion: It is necessary for grass-roots hospitals to carry out some comprehensive measures to improve clinical pharmacists' professional quality and general ca-pacity, promote the consummation of rules and regulations, and enhance cognition of medical staffs to clinical pharmacy practice and clinical pharmacists so as to promote the development of clinical pharmacy practice, which is helpful in training better clinical pharma-cists.