1.Research progress on palmitoylation of postsynaptic density protein 95
Yinghan ZHANG ; Honghong ZHANG ; Eryan KONG ; Zhongjian ZHANG ; Xiaoning WANG
Chinese Journal of Geriatrics 2018;37(12):1423-1427
Postsynaptic density protein 95(PSD-95) ,the most abundant and significant synapse scaffolding protein at excitatory synapses ,is a hallmark of synaptic function. Proteins palmitoylation is one of the important post-translational modifications ,which is occurred more frequently in the nervous system than in other organs. Palmitoylation-dependent regulation of PSD-95 has become a research hotspot in the fields of neuroplasticity and neuropsychiatric diseases.
2.Effects of cattle encephalon glycoside and ignotin on the expression of glial fibrillary acidic protein and neuronal nuclear antigen in the brain of the APP/PS1 mouse models of Alzheimer's disease
Yinghan ZHANG ; Yazhuo HU ; Zhitao HAN ; Ya GAO ; Ruisheng LI ; Eryan KONG ; Xiaoning WANG ; Zhongjian ZHANG ; Honghong ZHANG
Chinese Journal of Geriatrics 2020;39(9):1067-1071
Objective:To investigate the effects of cattle encephalon glycoside and ignotin(CEGI)on the expression of glial fibrillary acidic protein(GFAP)and neuronal nuclear antigen(NeuN)in the brain of APP/PS1 model mice of Alzheimer's disease.Methods:A total of 36 5-month-old APP/PS1 dual-transgenic model mice were randomly divided into 3 groups: the model group(normal saline 6.6 ml·kg -1·d -1), CEGI group(CEGI 6.6 ml·kg -1·d -1)and donepezil group(donepezil 2 mg·kg -1·d -1), with 12 in each group.Twelve C57BL/6J mice of the same age were used as the normal control group.All mice were given drugs for 6 weeks consecutively.Brain tissue was collected for immunohistochemical staining to detect the expression of amyloid β-protein(Aβ), GFAP and NeuN, which were then analyzed quantitatively. Results:The results of immunohistochemical staining indicated that levels of Aβ and GFAP were higher and levels of NeuN were lower in the model group than in the normal control group(0.147±0.068% vs.0%, 61.750±22.020 vs.26.000±4.598, 0.021±0.002 vs.0.032±0.003, P<0.05). Levels of Aβ and GFAP were lower and levels of NeuN were higher in the CEGI group and the donepezil group than in the model group(0.058±0.055 % vs.0.057±0.045 %, 38.250±5.418 vs.36.130±5.963, 0.029±0.004 vs.0.027±0.003, P<0.05). There was no significant difference in the expression of Aβ, GFAP and NeuN between the CEGI group and the donepezil group( P>0.05). Conclusions:CEGI has multi-target neuroprotective effects via down-regulating the expression of Aβ and GFAP and up-regulating the expression of NeuN.