Objective To establish a rate model of stable orthotopic liver transplantation using three-cuff technique.Methods 28 dead rats were dissected and the causes for death and the complication analyzed after liver transplantation using three-cuff method.Results The main postoperative complications included:bleeding,stenosis of liver superior vena cava,portal venous thrombus,respiration duct obstruction,gastrectasia,pneumothorax,adhesion of liver to diaphragm.Conclusion Most of 8 kinds of complications were related with operation.

Objective To study effects of the intrathymic injection of allohepatic cells on intestinal allograft.Methods Adult male DA and PVG rats,7-9 week old,were used as donors and recipients,respectively.The ectopic small howel transplantations were performed one week after intrathymic injection of hepatic cells without use of any immunosuppressants.The surgical procedures were carried out in a such manner that portal vein was anastomosed with 1eft renal vein by the cuff,aorta was anastomosed with superior mesenteric artery with its aortic patch.Results The donor liver ceils could survive in the recipient thymus for 3 weeks.The small intestinal allograft showed severe rejection and donor died one week after intrathymic injection of hepatic cells,but the hepatic cells could survive within the thymus at this time.Conclusion The intrathymic injection of allohepatic cells could not induce immune tolerance of the recipient to the same donor small intestinal graft.