Objective To determine pathogenesis and the suitable time of operation for myelomeningocele associated with hydrocephalus in neonate.Methods 6 underwent head CT scanning, 2 lumbosacral CT scanning and 6 lumbosacral X radiography on 6 patients myelomeningocele complicated with hydrocephalus.Ventriculoperitoneal shunt and repair of the myelomeningocele were performed respectively for one patient.from 1 day to 28 day old.Operation stage 1 in 5 patients.Repair of the myelomeningocele concurred with ventriculoperitoneal shunt. Intracranial pressure was measured in shunting procedure.Results 4 patients had normal intracranial pressure,2 patients increased intracranial pressure in the 6 patients.The volume of the hernial sac had markedly diminished and status of hernial sac had greatly improved wall in the patient who wnderwent two-stage procedures after shunt procedure. Lumbosacral wound healing was good . No recurrent myelomeningocele was found, no hydrocephalus was seen using head CT scanning and clinical manifestation has improved in these patients who were followed up 6 month to 3 year.Conclusions Hydrocephalus may deteriorate the degree of lumbosacral myelomeningocele. Effecacy of vntriculoperitoneal shunt and repair of the myelomeningocele was excellent in myelomeningocele complicated with hydrocephalus in neonate.Micro-operative technique might prevent the occurrence of tethered cord.

Objective To explore the peripheral blood T lymphocytes cell level in different grades of cerebral gliomas pa-tients at the time of before and after operation and its clinical significance .Methods A total of 80 cases of brain tumor patients from February 2010 to February 2012 in this hospital were chosed as study objects ,included 57 cases of glioma ,23 cases of other brain tumors ;57 cases of glioma were divided into 19 cases of low grade group and 38 cases of high grade group accorded to WHO grading standards ,and 50 cases of healthy people in same period were selected as control group .Venous blood in three groups were extracted at 1st day and 1st week after operation ,to detected the level of T lymphocyte subsets in peripheral blood and analyze the relationship between the different grade gliomas and prognosis .Results The peripheral blood CD3 + ,CD4 + ,CD4 + /CD8 + in control group were significantly higher than that of other brain tumor group and the glioma group ,CD8 + was significantly lower than that of the two groups(P< 0 .05) .Compared with other tumor group ,peripheral blood CD3 + ,CD4 + ,CD4 + /CD8 + in glioma group de-creased significantly ,CD8 + increased significantly(P< 0 .05) ;before and after the operation ,the CD3 + and CD4 + /CD8 + levels were significantly higher in low grade group than in the high grade group ,CD8 + was significantly lower in low grade group than in the high grade group(P< 0 .05) ,at the time of 1st week after operation ,CD3 + and CD4 + /CD8 + increased ,CD8 + decreased significant-ly of two groups when intra-group comparison (P < 0 .05) .The median survival time were 31 months in low grade group and 13 months in high grade group ,and the singnificance was found in two groups(P< 0 .05) .The median survival time were 34 months in CD4 + /CD8 + > 1 group and 17 months in CD4 + /CD8 + < 1 group ,the singnificance was found in two groups(P< 0 .05) .Conclusion The immune function of patients with brain glioma is inhibited ,the higher the malignancy of the tumor ,the more obvious of im-mune inhibition ;T lymphocyte subsets level could be used as evaluating index of malignant degree and prognosis of brain glioma .

Objective To study the correlation between serum thioredoxin 1(TRX-1)and nucleotide-bind-ing oligomerization domain-like receptor protein 3(NLRP3)expression levels and functional dyspepsia(FD)in children.Methods A total of 45 children with FD who were admitted to Shijiazhuang Maternal and Child Health Hospital from May 2022 to June 2023 were enrolled as the FD group.Another 40 healthy children were selected as the control group.Real-time fluorescence quantitative PCR was used to measure the expression lev-els of serum TRX-1 and NLRP3 mRNA.Spearman correlation analysis was used to analyze the correlation be-tween the expression levels of serum TRX-1 mRNA,NLRP3 mRNA and FD related scores.Multivariate Lo-gistic regression was used to analyze the influencing factors of FD.Results The expression levels of TRX-1 mRNA and NLRP3 mRNA in FD group were higher than those in control group(P＜0.05).The mild and moderate FD groups had significantly lower serum TRX-1 mRNA and NLRP3 mRNA expression levels than the severe FD group,and the mild FD group had significantly lower serum TRX-1 mRNA and NLRP3 mRNA expression levels than the moderate FD group(P＜0.05).The expression levels of TRX-1 mRNA and NLRP3 mRNA in serum were positively correlated with egigastric pain,abdominal distension or epigastric discomfort,nausea and vomiting(P＜0.05),but were not correlated with early satiety,loss of appetite or food intake,and belching(P＞0.05).The expression level of serum NLRP3 mRNA was positively correlated with the total score(P＜0.05),while the expression level of serum TRX-1 mRNA was not correlated with the total score(P＞0.05).The increased levels of serum TRX-1 mRNA and NLRP3 mRNA were independent risk factors for FD(P＜0.05).Conclusion The expression levels of TRX-1 and NLRP3 may play an important role in the pathogenesis of FD.

Objective To investigate the changes and clinical significance of serum monocyte chemoattractant protein-1(MCP-1)and prostaglandin E2(PGE2)levels in children with functional dyspepsia(FD).Methods A retrospective study was conducted to enroll 89 FD children admitted from October 2022 to October 2023 as FD group,and 91 healthy children in the same period were se-lected as control group.The FD group was further divided into mild group(n=36),moderate group(n=33),and severe group(n=20)based on the severity of the disease.The serum levels of MCP-1 and PGE2 were detected by enzyme-linked immunosorbent assay(ELISA).The severity of the disease was assessed by the Global Overall Symptom Score(GOSS),and gastric emptying was evaluated in all the children.Pearson and Spearman correlation analyses were performed to explore the correlations of serum MCP-1 and PGE2 levels with gastric half-emptying time and FD symptom scores.Results The serum MCP-1 level in the FD group was significantly higher than that in the control group,while the PGE2 level was significantly lower(P＜0.05).In the severe group,the serum MCP-1 level was significantly higher while the PGE2 level was significantly lower than that in the moderate and mild groups,and similar differences were significantly observed between the moderate and mild groups(P＜0.05).The gastric half-emptying time and postprandial 2-hour residual rate were significantly higher in the FD group than the control group(P＜0.05).Serum MCP-1 in FD children was posi-tively correlated with gastric half-emptying time and postprandial 2-hour residual rate(P＜0.05),while serum PGE2 was negatively correlated with these parameters(P＜0.05).Serum MCP-1 in FD children was significantly positively correlated with upper abdominal burning sensation,early satie-ty,belching,upper abdominal pain,and the total GOSS score(r=0.611,0.509,0.504,0.491,0.513;P＜0.05),while PGE2 was significantly negatively correlated with upper abdominal burn-ing sensation,early satiety,belching,upper abdominal pain,acid reflux,and GOSS total score(r=-0.516,-0.561,-0.493,-0.533,-0.497,-0.482;P＜0.05).Conclusion Ele-vated serum MCP-1 and decreased PGE2 levels in children with FD are closely related to the severity of the disease,gastric emptying,and some FD symptoms.

Objective To investigate the changes and clinical significance of serum monocyte chemoattractant protein-1(MCP-1)and prostaglandin E2(PGE2)levels in children with functional dyspepsia(FD).Methods A retrospective study was conducted to enroll 89 FD children admitted from October 2022 to October 2023 as FD group,and 91 healthy children in the same period were se-lected as control group.The FD group was further divided into mild group(n=36),moderate group(n=33),and severe group(n=20)based on the severity of the disease.The serum levels of MCP-1 and PGE2 were detected by enzyme-linked immunosorbent assay(ELISA).The severity of the disease was assessed by the Global Overall Symptom Score(GOSS),and gastric emptying was evaluated in all the children.Pearson and Spearman correlation analyses were performed to explore the correlations of serum MCP-1 and PGE2 levels with gastric half-emptying time and FD symptom scores.Results The serum MCP-1 level in the FD group was significantly higher than that in the control group,while the PGE2 level was significantly lower(P＜0.05).In the severe group,the serum MCP-1 level was significantly higher while the PGE2 level was significantly lower than that in the moderate and mild groups,and similar differences were significantly observed between the moderate and mild groups(P＜0.05).The gastric half-emptying time and postprandial 2-hour residual rate were significantly higher in the FD group than the control group(P＜0.05).Serum MCP-1 in FD children was posi-tively correlated with gastric half-emptying time and postprandial 2-hour residual rate(P＜0.05),while serum PGE2 was negatively correlated with these parameters(P＜0.05).Serum MCP-1 in FD children was significantly positively correlated with upper abdominal burning sensation,early satie-ty,belching,upper abdominal pain,and the total GOSS score(r=0.611,0.509,0.504,0.491,0.513;P＜0.05),while PGE2 was significantly negatively correlated with upper abdominal burn-ing sensation,early satiety,belching,upper abdominal pain,acid reflux,and GOSS total score(r=-0.516,-0.561,-0.493,-0.533,-0.497,-0.482;P＜0.05).Conclusion Ele-vated serum MCP-1 and decreased PGE2 levels in children with FD are closely related to the severity of the disease,gastric emptying,and some FD symptoms.